Metabolomics-based study of the effect of dietary N-carbamoylglutamic acid addition to heifers in late pregnancy on newborn calves.

It has been demonstrated that supplementing late-gestation cow diets with NCG (N-carbamoylglutamic acid) increases the serum protein level, boosts immunological function, and increases the birth weight of the calves. However, the underlying mechanism remains unclear. In this experiment, 30 late-gestation Angus heifers almost at same conditions were chosen for this experiment.

Dynamic changes of rumen bacteria and their fermentative ability in high-producing dairy cows during the late perinatal period.

Background: High-producing dairy cows face varying degrees of metabolic stress and challenges during the late perinatal period, resulting in ruminal bacteria abundance and their fermentative ability occurring as a series of changes. However, the dynamic changes are still not clear.

Aims/methods: Ten healthy, high-producing Holstein dairy cows with similar body conditions and the same parity were selected, and ruminal fluid from the dairy cows at postpartum 0, 7, 14, and 21 d was collected before morning feeding.

Influence of Parturition on Rumen Bacteria and SCFAs in Holstein Cows Based on 16S rRNA Sequencing and Targeted Metabolomics.

The rumen fluids from ten cows at Day 3~5 before calving and Day 0 after calving were collected to analyze the composition and quantity of bacterial communities and concentrations of SCFAs. The results showed that the relative abundances of unidentified and genera were significant increased ( < 0.05), while that of unidentified was notably decreased after calving ( < 0.

Integrated Metabolomics and Transcriptome Revealed the Effect of Fermented Residue Promoting Immunity.

residue contains abundant bioactive nutrients which can be used as feed supplement. The fermentation treatment of plant residue can promote the utilization of nutrients, rumen digestion, and the growth and immunity of animals. Based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabolomics and in-depth transcriptome analysis, the study tested the mechanisms of residue (RW) and fermented residue (RFW) on meat quality and immunity of sheep.

[Establishment of a quality evaluation method for Angelica different processed products from genuine producing areas based on data mining].

The paper reports the development of a quality evaluation method for Angelica different processed products. The data of high-performance liquid chromatography, water, total ash and extract were analyzed with SPSS Clementine 11.0 software.

[Study on quality standard of oil-processed Radix Angelica sinensis].

Objective: To establish the quality standard of oil-processed Radix Angelica sinensis.

Methods: Combined traditional identification, TLC and fingerprints of wine-processed Radix Angelica sinensis to control quality of oil-processed Radix Angelica sinensis. And referring to China Pharmacopoeia of 2005 edition, water, ash, and extract were also detected.

[Quality control and discrimination of angelica different processed products based on HPLC fingerprints combined chemometrics methods].

Objective: To establish a chemical fingerprint method for reorganizing and validating angelica different processed products.

Method: A high-performance liquid chromatographic method was developed to establish the fingerprint. Principal component analysis, hierarchical cluster analysis and discriminate analysis were applied to study HPLC finger printing and chemical pattern reorganization.