[A multicenter survey of antibiotic use in very and extremely low birth weight infants in Hunan Province].

Objective: To investigate the current status of antibiotic use for very and extremely low birth weight (VLBW/ELBW) infants in neonatal intensive care units (NICUs) of Hunan Province.

Methods: The use of antibiotics was investigated in multiple level 3 NICUs of Hunan Province for VLBW and ELBW infants born between January, 2017 and December, 2017.

Results: The clinical data of 1 442 VLBW/ELBW infants were collected from 24 NICUs in 2017.

A simple method for determining compound affinity and chemical yield from DNA-encoded library selections.

DNA-encoded libraries (DELs) can contain billions of unique chemical species; selecting against such large inputs, it is typical to find more candidate binders than is reasonable to pursue for follow-up synthesis and testing. Given this wealth of choices, common practice is to limit synthesis to only those compounds estimated to have the greatest chance of being high-affinity binders; of the many potential factors contributing to this estimation, the strength of the selection signal of a candidate binder is always important. We define here methods and equations which relate the theoretical selection signal of a compound to its affinity and chemical yield.

Synthesis of Isomalto-Oligosaccharides by Pichia pastoris Displaying the Aspergillus niger α-Glucosidase.

We explored the ability of an Aspergillus niger α-glucosidase displayed on P. pastoris to act as a whole-cell biocatalyst (Pp-ANGL-GCW61) system to synthesize isomalto-oligosaccharides (IMOs). IMOs are a mixture that includes isomaltose (IG), panose (P), and isomaltotriose (IG).

Alleviates Ethanol-Induced Injury on Gastric Epithelial Cells via Downregulation of the NF-kB Signaling Pathway.

We used human gastric epithelial cells (GES-1) line in an ethanol-induced cell damage model to study the protective effect of and its modulation to NF-B signal pathway. The goal was to probe the molecular mechanism of decoction in the prevention of gastric ulcer and therefore provide guidance in the clinical application of on treating injuries from chronic nephritis, pleural effusion, gastric ulcer, and other ailments. The effects of -loaded serums on cell viability were detected by MTT assays.

Synthesis of octyl-β-D-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system.

We explored the ability of a Thai rosewood β-glucosidase-displaying P. pastoris whole-cell biocatalyst (Pp-DCBGL) system to synthesize alkyl β-D-glucosides. The primary investigation centered on the synthesis of octyl-β-D-glucopyranoside (octyl-glu, OG).

Scaling-up the synthesis of myristate glucose ester catalyzed by a CALB-displaying Pichia pastoris whole-cell biocatalyst.

The novel whole-cell biocatalyst Candida antarctica lipase B displaying-Pichia pastoris (Pp-CALB) is characterized by its low preparation cost and could be an alternative to the commercial immobilized Candida antarctica lipase B (CALB). This study addresses the feasibility of using Pp-CALB in large scale glucose fatty acid esters production. 1,2-O-Isopropylidene-α-D-glucofuranose (IpGlc) was used as the acyl acceptor to overcome the low solubility of glucose in an organic solvent and to avoid the addition of toxic co-solvents.

[Impact factors analysis of standardized perioperative antibiotic application on nosocomial infection and drug-resistance bacteria].

Objective: To investigate the impact factors and clinical significance of standardized perioperative antibiotic application on nosocomial infection and drug-resistant bacteria strains in eye hospital.

Methods: It was a retrospective series case study. The inpatients underwent ophthalmologic operation of one year before standardized application (from Sep.

Optical coherence tomographic features in a case of bilateral macular coloboma with strabismus.

Purpose: To report the optical coherence tomography (OCT) findings in a patient with bilateral macular coloboma with strabismus.

Methods: A 21-year-old male presented with macular coloboma in both eyes.Fundus photography, fundus fluorescence angiography (FFA) and OCT were performed.

[Characters of multifocal electroretinogram in different grades of diabetic macular edema].

Objective: To investigate the characters of multifocal electroretinogram (mfERG) in different grades of diabetic macular edema defined by optical coherence tomography (OCT).

Methods: MfERG and OCT were performed in 57 eyes of diabetic macular edema (DME) patients and 35 eyes of the control group. According to the macular thickness measured by OCT, eyes with DME were divided into three groups: mild, moderate and severe DMEs.

Effect of CO(2) laser sclerectomy with iridectomy on ocular hypertension in rabbits.

Aim: To evaluate the efficiency and safety of an optimized CO(2) Laser glaucoma surgery system for laser sclerectomy with iridectomy.

Methods: Rabbit trials were performed to evaluate the efficiency and safety.

Results: IOP was significantly decreased in laser group compared with trabeculectomy group(P<0.

F-heparin modified intraocular lenses in Rhesus monkeys.

Aim: In order to improve the biocompatibility of intraocular lenses (IOL), the polymethylmethacrylate (PMMA) IOL was modified with F-heparin.

Methods: The PMMA IOL was modified with F ions and heparin by the technique of ion beam combined with low temperature and low pressure plasma. The monkeys (20 eyes) with cataract partly were randomly classified into 2 groups and implanted with PMMA IOL and modified IOL respectively for 180 days.

[An analysis of adhered cells on the surface of F-heparin surface modified intraocular lenses implanted into rhesus monkeys' eyes].

Objective: To study the biocompatibility of F-heparin surface modified intraocular lenses (IOLs) by observing cellular reaction on the surface of IOLs implanted into the rhesus monkeys' eyes.

Methods: Ten monkeys (20 eyes) were classified into two groups. PMMA IOLs and F-heparin modified IOLs were respectively implanted into 10 monkeys' eyes.