Evaluating resistance of potato cultivars and breeding clones to golden cyst nematode in China.

The golden cyst nematode, Globodera rostochiensis, is a regulatory pathogen that can significantly reduce potato yields and hinder the international trade of potatoes. Unfortunately, this nematode has been reported in southwestern China. Nevertheless, limited information exists on the resistance levels of Chinese potato germplasm, especially primary commercial cultivars, to G.

An assessment of clinical reception training using standard patient and dental simulator in prosthodontic dentistry for dental undergraduates: a historical control trial.

Background: Clinical reception training plays a crucial role in developing undergraduates' clinical thinking and competence. The study aimed to evaluate the effectiveness of clinical reception training conducted by standard patients (SPs) and dental simulators among undergraduate students.

Materials And Methods: In the first week of the internship, sixty-five 5-year undergraduate students were divided into two groups: SP group, which received traditional theoretical training along with clinical reception training, and control group that only received traditional theoretical training.

The m6A reader IGF2BP1 contributes to the activation of hepatic stellate cells through facilitating TUBB4B mRNA stabilization.

The m6A reader insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) is involved in multiple pathophysiological processes through enhanced expression of the proteins encoded by their target mRNAs. However, the functional role of IGF2BP1-mediated m6A in liver fibrosis remains elusive. Here, we report that IGF2BP1 is highly expressed in activated hepatic stellate cells (HSCs), the major driver of fibrogenesis, and TUBB4B is identified as a potential target of IGF2BP1 by re-analysis of the RNA-seq, RIP-seq, and m6A-seq data.

Preparation of magnetic activated carbon fibers@FeO by electrostatic self-assembly method and adsorption properties for methylene blue.

Nano-FeO was loaded onto coconut-based activated carbon fibres (CACF) using an electrostatic self-assembly method. The effects of the mass ratio of CACF to nano-FeO, loading time, pH and temperature on the loading effect were investigated and ideal loading conditions were determined. To study the adsorption performance of MACF@FeO for methylene blue, the effects of the initial concentration, pH and time on the adsorption were investigated and the working conditions of adsorption were established.

GT-CAM: Game Theory Based Class Activation Map for GCN.

Graph Convolutional Networks (GCN) have shown outstanding performance in skeleton-based behavior recognition. However, their opacity hampers further development. Researches on the explainability of deep learning have provided solutions to this issue, with Class Activation Map (CAM) algorithms being a class of explainable methods.

Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes.

The role of Galectin-9 (Gal-9) in the pathogenesis of rheumatoid arthritis (RA) remains unclear. This study aimed to investigate the mechanism of action and therapeutic potential of Gal-9 in RA. We detected Gal-9 expression in clinical samples, explored the mechanism of function of Gal-9 by knockdown and overexpression in fibroblast-like synoviocytes (FLSs), and further verified it in collagen-induced arthritis (CIA) model.

The combination of tetracyclines effectively ameliorates liver fibrosis via inhibition of EphB1/2.

Eph receptor tyrosine kinase EphB1/2 contributes to the development of liver fibrosis, suggesting the rationale that EphB1/2 inhibitors may be effective in liver fibrosis therapy. Since tetracycline antibiotics were recently demonstrated as EphB kinase inhibitors, in present study we investigated their therapeutic potential against liver fibrosis. Our results showed that the tetracycline combination of demeclocycline (D), chlortetracycline (C), and minocycline (M) inhibited the activation of hepatic stellate cells (HSCs) in vitro and alleviated CCl-induced animal model of liver fibrosis in vivo.

Association between serum copper-zinc ratio and respiratory tract infection in children and adolescents.

Background: The aim of this study was to explore the association between serum copper-zinc (Cu-Zn) ratio and the risk of respiratory tract infection in children and adolescents.

Methods: This cross-sectional study collected the data of 1695 participants who aged 6-17 years with follow-up data on respiratory tract infection in 2011-2012, 2013-2014 and 2015-2016 cycles from the National Health and Nutrition Examination Survey (NHANES) database. Univariate logistic regression analysis was applied to explore the covariates.

Application of virtual technology to maximize esthetics and function in the restoration of anterior traumatic dental injuries.

The restoration of multiple teeth with traumatic injury in the esthetic zone is complex. For the present patient, an intraoral scanner, a facial scanner, a jaw motion analyzer, and cone beam computed tomography were applied to collect patient data and establish a virtual dental patient. The virtual technology increased the accuracy of tooth- and implant-supported crowns in both appearance and occlusion.

Comparison of case-based learning combined with Rain Classroom teaching and traditional method in complete denture course for undergraduate interns.

Background: Complete denture, as an important restoration method for edentulism, is difficult to study for beginners, especially in linking the theory with clinical practice.

Objective: This study was aimed to compare the teaching effects between case-based learning combined with Rain Classroom teaching and traditional lecture method in the clinical course of complete denture prosthesis for undergraduate interns.

Methods: In a course called "Problems and treatment strategies of complete denture after wearing", interns were divided into two groups: one for traditional lecture-based teaching with PowerPoint slideshow (the control group, n = 28); and the other for case-based learning combined with Rain Classroom teaching, which published information before class, discussed specific clinic cases in class and got real-time interns' feedback via WeChat (the test group, n = 22).

circFOXM1 contributes to sorafenib resistance of hepatocellular carcinoma cells by regulating MECP2 via miR-1324.

As one of the most common malignant tumors, hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths around the world. Emerging studies have indicated that circular RNAs (circRNAs), which play a crucial role in HCC pathogenesis and metastasis, are differentially expressed in HCC. However, the regulatory mechanisms of circRNA on sorafenib resistance of HCC are still unknown.

Effect of hydroxychloroquine on angiographic progression in routine treatment of Takayasu arteritis.

Objectives: Hydroxychloroquine (HCQ), an anti-malarial drug, is widely used in the treatment of rheumatic diseases. However, the benefits of HCQ in the treatment of Takayasu arteritis (TA) remain unclear, especially in terms of alleviation of vascular progression.

Methods: This longitudinal observational retrospective study was based on the East China TA cohort.

Human umbilical cord mesenchymal stem cells-derived exosomal microRNA-451a represses epithelial-mesenchymal transition of hepatocellular carcinoma cells by inhibiting ADAM10.

Exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs) expressing microRNAs (miRNAs) have been highlighted in human cancers. However, the detailed molecular mechanism of hucMSCs-derived exosomal miR-451a on hepatocellular carcinoma (HCC) remains further investigation. Our study aims to explore the impact of exosomal miR-451a on the progression of HCC.

Expression of Notch-Hif-1α signaling pathway in liver regeneration of rats.

Objective: To investigate whether the Notch-Hif-1α signaling pathway is involved in liver regeneration.

Methods: Rats were divided into two groups and treated with daily intraperitoneal injections of saline (control) or the gamma-secretase inhibitor, Fli-06, for 2 days. Two-thirds of the rat livers were resected and rats were later euthanized at specific time points post-resection to analyze the remnant livers.

The Construction of Biomimetic Cementum Through a Combination of Bioskiving and Fluorine-Containing Biomineralization.

Despite tremendous attention is given to the construction of biomimetic cementum for regeneration of tooth cementum, the lack of recapitulating the composition and hierarchical structure of cementum often leads to the poor performance of constructed materials. How to highly mimic the sophisticated composition and hierarchy of cementum remains a longstanding challenge in constructing the biomimetic cementum. Inspired by cementum formation process, a novel construction approach via a combination of bioskiving and fluorine-containing biomineralization is developed in this study.

Selective targeting of the oncogenic G12S mutant allele by CRISPR/Cas9 induces efficient tumor regression.

: is one of the most frequently mutated oncogenes in cancers. The protein's picomolar affinity for GTP/GDP and smooth protein structure resulting in the absence of known allosteric regulatory sites makes its genomic-level activating mutations a difficult but attractive target. : Two CRISPR systems, genome-editing CRISPR/SpCas9 and transcription-regulating dCas9-KRAB, were developed to deplete the G12S mutant allele or repress its transcription, respectively, with the goal of treating -driven cancers.

Comparison of osteogenic differentiation induced by and delivered by lipopolysaccharide-amine nanopolymersomes and underlying molecular mechanisms.

Gene therapies via small interfering (si)RNA () and bone morphogenetic protein () plasmid DNA () may be promising strategies for bone repair/regeneration, but their ideal delivery vectors, efficacy difference, and underlying mechanisms have not been explored, so these issues were probed here. This study used lipopolysaccharide-amine nanopolymersomes (LNPs), an efficient cytosolic delivery vector developed by the research team, to mediate and to transfect MC3T3-E1 cells, respectively. The cytotoxicity, cell uptake, and gene knockdown efficiency of -loaded LNPs (LNPs/) were studied, then the osteogenic-differentiation efficacy of MC3T3-E1 cells treated by LNPs/ and LNPs/, respectively, were compared by measuring the expression of osteogenesis-related genes and proteins, alkaline phosphatase (ALP) activity, and mineralization of the extracellular matrix at all osteogenic stages.

Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells.

The effects of long intergenic non-coding ribonucleic acid (lincRNA)-p21 targeting hypoxia-inducible factor-1α (HIF-1α) on proliferation, apoptosis and migration of liver cancer cells were investigated. MHCC97H liver cancer cells were infected with control lentivirus (control group) and lincRNA-p21 lentivirus (observation group), and control stable cell lines and lincRNA-p21 stable cell lines were screened and obtained by using puromycin. The expression levels of lincRNA-p21 messenger RNA (mRNA) in the control and observation groups were analyzed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

MiR-124 sensitizes cisplatin-induced cytotoxicity against CD133 hepatocellular carcinoma cells by targeting SIRT1/ROS/JNK pathway.

Drug resistance is still a major obstacle for efficient treatment of hepatocellular carcinoma (HCC) during the cisplatin-based chemotherapy. Recent studies have demonstrated that CD133 positive population of cancer cells are responsible for multiple drug resistance. We are supposed to take strategies to sensitize CD133 HCC cells to cisplatin treatment.

Metal-organic frameworks-derived MnO/MnO microcuboids with hierarchically ordered nanosheets and TiC MXene/Au NPs composites for electrochemical pesticide detection.

Transition metal oxides (TMOs) derived from metal - organic frameworks (MOF) combined with two-dimensional (2D) transition metal carbides possibly pave an innovative pathway for designing promising biosensors. Herein, a novel electrochemical sensing platform has been fabricated for ultra-sensitive determination of organophosphorus pesticides (OPs), based on MOF-derived MnO/MnO and TiC MXene/Au NPs composites. Remarkably, the three-dimensional (3D) MnO/MnO hierarchical microcuboids derived from Mn-MOF are composed of vertically aligned, highly ordered nanosheets, and further combined with MXene/Au NPs yields synergistic signal amplification effect, with outstanding electrochemical performance, large specific surface area, and good environmental biocompatibility.

High melatonin levels are related to spinal ossification in patients with ankylosing spondylitis.

To investigate associations of serum melatonin with spinal ossification and cytokines in ankylosing spondylitis (AS). Serum was obtained from 52 AS patients and 25 healthy controls. Melatonin was measured by ELISA kit; bone morphogenetic protein (BMP)-2, dickkopf-related protein (Dkk)-1, IL-1β, IL-6, IL-17 and TNF-α concentrations were assayed using Luminex multiplex bead system.

Clinical characteristics of rheumatoid arthritis patients with peripheral neuropathy and potential related risk factors.

Objectives: To investigate potential risk factors of peripheral neuropathy (PN) in rheumatoid arthritis patients (RA).

Methods: Eighty-eight patients with RA were enrolled in this study, including patients with PN (n = 44; 28 patients with multiple nerves (MN) involvement and 16 patients with single nerve (SN) involvement) and without (n = 44) peripheral neuropathy were enrolled. Their clinical features were comprehensively collected including symptoms/signs, lab results, electromyogram data.