A facile way to fabricate a thrombin immobilized composite sponge with dual hemostatic effects for acute hemorrhage control.

Uncontrolled bleeding and excessive blood loss stand as the leading causes of death in complex surgeries, civilian traumas, and military operations. Sponges have been used for developing efficiency hemostats, but most commonly used hemostatic sponges possess only one single coagulation mechanism or lack inherent blood clotting ability. Herein, we proposed simple yet innovative approaches for creating novel hemostatic composite sponges with dual hemostatic effects.

Damage evolution law and failure mechanism of rock impacted by high-pressure water jet under in-situ stress condition.

To investigate the real physical mechanism of rock fragmentation subjected to water jet under in-situ stress condition, a numerical model based on the SPH algorithm was established using the rate-dependent constitutive model to simulate the rock-breaking process. First, the damage evolution law of rock impacted by high-pressure water jet under in-situ stress conditions was studied by analyzing the distribution characteristics of the damage field in the dynamic process of water jet impinging. The results showed that the damage field, widths of surface damage, maximum widths of damage and mean depths of damage of rock decreased with the increase of in-situ stress, indicating that the existence of initial in-situ stress had a strong inhibitory effect on rock fragmentation.

Stability analysis of rock slope and calculation of rock lateral pressure in foundation pit with structural plane and cave development.

In this study, numerical simulations were carried out to analyze the influence of caves in different positions and shapes, in combination with structural planes, on the stability of the slope and the failure characteristics of a rock slope in a deep foundation pit with high inclination structural planes and cave development. The schemes for substituting a single karst cave for karst caves were constructed. Based on the penetration failure characteristics of karst caves between parallel structural planes, calculation methods for the safety factor of the rock foundation pits and the upper bound of the lateral pressure of supporting structures under the combined influence of the caves and structural planes have been developed, which can be used to assess the safety factor of a rock mass and to calculate the lateral pressure under complex geological conditions.

A novel bioactive polyurethane with controlled degradation and L-Arg release used as strong adhesive tissue patch for hemostasis and promoting wound healing.

Effective strategy of hemostasis and promoting angiogenesis are becoming increasingly urgent in modern medicine due to millions of deaths caused by tissue damage and inflammation. The tissue adhesive has been favored as an optimistic and efficient path to stop bleeding, while, current adhesive presents limitations on wound care or potential degradation safety in clinical practice. Therefore, it is of great clinical significance to construct multifunctional wound adhesive to address the issues.

In-situ self-assembly of bacterial cellulose/poly(3,4-ethylenedioxythiophene)-sulfonated nanofibers for peripheral nerve repair.

Biocompatible and electroactive biomaterials have good potential on peripheral nerve repair. Bacterial cellulose (BC) shows excellent biocompatibility and is easy to modified, however it lacks electroactivity. In this study, biocompatible, conductive, and transparent bacterial cellulose/poly(3,4-ethylenedioxythiophene)-sulfonated nanofibers (BC/PEDOT-SNFs, BPS) composite membranes were prepared through the in-situ polymerization of PEDOT and the doping of SNFs.

Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo.

Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits (NGCs). However, problems such as low loading efficiency and cell anoikis undermine the outcomes. Microcarriers are efficient 3D cell culture scaffolds, which can also prevent cell anoikis by providing substrate for adhesion during transplantation.

Expressions of CD44, PCNA and MRP1 in lung cancer tissues and their effects on proliferation and invasion abilities of lung cancer cell line 95D.

Purpose: To investigate the expressions of CD44 non-small cell lung cancer cells, proliferating cell nuclear antigen (PCNA) and multidrug resistance-associated protein 1 (MRP1) in the lung cancer tissues and their effects on the proliferation and invasion abilities in vitro of lung cancer cell line 95D.

Methods: 138 lung cancer tissues and 127 adjacent normal tissues were collected from lung cancer patients after operation in Shandong Provincial Third Hospital from January 2015 to December 2017. CD44 siRNA (experimental CD44 group), PCNA siRNA (experimental PCNA group) and MRP1 siRNA (experimental MRP1 group) were transfected into human lung cancer 95D cells, and a negative control group (cells transfected with miR-Native Control) and a blank group (untransfected cells) were established.

Cellulose fibers-reinforced self-expanding porous composite with multiple hemostatic efficacy and shape adaptability for uncontrollable massive hemorrhage treatment.

Uncontrollable hemorrhage leads to high mortality and thus effective bleeding control becomes increasingly important in the military field and civilian trauma arena. However, current hemostats not only present limitation when treating major bleeding, but also have various side effects. Here we report a self-expanding porous composites (CMCP) based on novel carboxymethyl cellulose (CMC) fibers and acetalized polyvinyl alcohol (PVA) for lethal hemorrhage control.

Design and characterization of plasticized bacterial cellulose/waterborne polyurethane composite with antibacterial function for nasal stenting.

A nasal stent capable of preventing adhesions and inflammation is of great value in treating nasal diseases. In order to solve the problems of tissue adhesion and inflammation response, we prepared plasticized bacterial cellulose (BCG) and waterborne polyurethane (WPU) composite with antibacterial function used as a novel nasal stent. The gelation behavior of BCG could contribute to protecting the paranasal sinus mucosa; meanwhile, the WPU with improved mechanical property was aimed at supporting the narrow nasal cavity.

Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.

In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin. The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI. The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.

Comparison of the diagnostic value of CEA combined with OPN or DKK1 in non-small cell lung cancer.

Carcinoma embryonic antigen (CEA), osteopontin (OPN), and Dickkopf-1 (DKK1) expressed in serum are associated with hypoxia in tumor progression. However, the role of these proteins in the plasma of patients with non-small cell lung cancer (NSCLC) is poorly understood. The diagnostic values of CEA combined with OPN or DKK1 were compared in non-small cell lung cancer.

Words Can Shift: Dynamically Adjusting Word Representations Using Nonverbal Behaviors.

Humans convey their intentions through the usage of both verbal and nonverbal behaviors during face-to-face communication. Speaker intentions often vary dynamically depending on different nonverbal contexts, such as vocal patterns and facial expressions. As a result, when modeling human language, it is essential to not only consider the literal meaning of the words but also the nonverbal contexts in which these words appear.

Silver nanoparticles stimulate osteogenesis of human mesenchymal stem cells through activation of autophagy.

Previously, different results have been achieved regarding effects of silver nanoparticles (Ag NPs) on osteogenesis of stem cells and the mechanisms have not been disclosed yet, which are quite important for potential application of Ag NPs in bone reconstruction. Effects of Ag NPs on osteogenesis of human mesenchymal stem cells (hMSCs) with underlying mechanisms were investigated. Ag NPs at 2.

An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.

Anti-relapse therapy after surgery plays a critical role in cancer therapy. New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required. In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.

Highly transparent, highly flexible composite membrane with multiple antimicrobial effects used for promoting wound healing.

In recent years, bacterial cellulose (BC)-based dressings or patches for skin or soft tissue repair have become investigative emphasis. However, most of the BC-based products used for biomedical applications present limitations due to their low flexibility, poor gas permeability and no inherent antibacterial activity. Herein, we proposed and designed a novel composite composed of natural bacterial cellulose (BC), polyethylene glycol (PEG) and polyhexamethylene biguanidine (PHMB) through new synthetic approaches.

MiR-216b suppresses cell proliferation, migration, invasion, and epithelial-mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer.

MiR-216b and forkhead box M1 (FOXM1) were demonstrated to exert their biological effects on the development and progression of tumors. This study aimed to investigate the expression and role of miR-216b and FOXM1 in tissues and cell lines of non-small cell lung cancer (NSCLC). The expressions of miR-216b and FOXM1 in NSCLC tissues and cells were detected by qRT-PCR and Western blot analysis.

Esculetin enhances the inhibitory effect of 5-Fluorouracil on the proliferation, migration and epithelial-mesenchymal transition of colorectal cancer.

Background: Colorectal cancer (CRC) is the most common malignant disease worldwide and thus new therapeutic approaches are needed. 5-Fluorouracil (5-FU) remains the most widely used agent to treat colorectal cancer (CRC). However, its clinical efficacy is currently limited by the development of drug resistance.

In situ synthesis of bacterial cellulose/copper nanoparticles composite membranes with long-term antibacterial property.

Bacterial cellulose (BC), with unique structure and properties, has attracted much attention in the biomedical field, especially in using as wound dressing. However, pure BC lacks the antimicrobial activity, which limits its application in wound healing. To solve this problem, copper nanoparticles (Cu NPs) loaded BC membranes were fabricated by using in situ chemical reduction method.

Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.

With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention. In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection. This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.

Fabrication of nanofibrous microcarriers mimicking extracellular matrix for functional microtissue formation and cartilage regeneration.

Cartilage has rather limited capacities for self-repair and regeneration. To repair complexly shaped cartilage tissue defects, we propose the application of microtissues fabricated from bone marrow-derived mesenchymal stem cells (BMSCs) cultured in natural bionic nanofibrous microcarriers (NF-MCs). The NF-MCs were structurally and functionally designed to mimic natural extracellular matrix (ECM) by crosslinking dialdehyde bacterial cellulose (DBC) with DL-allo-hydroxylysine (DHYL) and complexing chitosan (CS) with DHYL through electrostatic interactions.

Compositions and Abundances of Sulfate-Reducing and Sulfur-Oxidizing Microorganisms in Water-Flooded Petroleum Reservoirs with Different Temperatures in China.

Sulfate-reducing bacteria (SRB) have been studied extensively in the petroleum industry due to their role in corrosion, but very little is known about sulfur-oxidizing bacteria (SOB), which drive the oxidization of sulfur-compounds produced by the activity of SRB in petroleum reservoirs. Here, we surveyed the community structure, diversity and abundance of SRB and SOB simultaneously based on 16S rRNA, and gene sequencing, and quantitative PCR analyses, respectively in petroleum reservoirs with different physicochemical properties. Similar to SRB, SOB were found widely inhabiting the analyzed reservoirs with high diversity and different structures.

A Comprehensive Meta-Analysis of MicroRNAs for Predicting Colorectal Cancer.

Colorectal cancer (CRC) has been defined as a common malignancy due to its prevailing incidence in both males and females. Recently, the intrinsic value of microRNAs (miRNAs) with respect to early cancer diagnosis has been contentious as the diagnostic accuracy of miRNAs significantly varied across different studies. As a result of this, this pioneer meta-analysis was proposed to address this issue.