Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM.

Three-dimensional structured illumination microscopy (3D-SIM) provides excellent optical sectioning and doubles the resolution in all dimensions compared with wide-field microscopy. However, its much lower axial resolution results in blurred fine details in that direction and overall image distortion. Here we present 4Pi-SIM, a substantial revamp of IS that synergizes 3D-SIM with interferometric microscopy to achieve isotropic optical resolution through interference in both the illumination and detection wavefronts.

Whether perceived limb heaviness affects return to work among patients with ischemic stroke.

This study aims to explore the relationship between perceived limb heaviness and return to work (RTW) among ischemic stroke patients. We retrospectively reviewed the data of consecutive participants recruited in the CIRCLE study. Perceived limb heaviness was gathered from self-reported by 1-year follow-up.

The role of Immune cells in Alzheimer's disease: a bidirectional Mendelian randomization study.

Background: Alzheimer's disease (AD) is a leading cause of dementia, characterized by the accumulation of amyloid-beta (Aβ) and hyperphosphorylated tau proteins, leading to neuroinflammation and neuronal damage. The role of the immune system in AD pathogenesis is increasingly recognized, prompting an exploration of the causal relationship between immune cells and AD by using Mendelian randomization (MR) approaches.

Methods: Utilizing genome-wide association study (GWAS) data from European cohorts, we conducted an MR study to investigate the causal links between immune cell phenotypes and AD.

The potential therapeutic roles of dental pulp stem cells in spinal cord injury.

Spinal cord injury (SCI) can lead to serious functional disorders, which have serious impacts on patients and society. The current traditional treatments of SCI are not effective the injured spinal cord is difficult to repair and regenerate. In recent years, stem cell transplantation for the treatment of SCI has been a hot research topic.

High-voltage pulsed radiofrequency improves ultrastructure of DRG and enhances spinal microglial autophagy to ameliorate neuropathic pain induced by SNI.

Neuropathic pain (NeP) is intractable for which many therapies are ineffective. High-voltage pulsed radiofrequency (HVPRF) on dorsal root ganglion (DRG) is considered an effective treatment for NeP. The aim of this study is to explore the therapeutic voltage for the optimal efficacy of PRF and the underlying mechanisms.

The controlling nutritional status score and risk factors associated with malnutrition in patients with acute ischemic stroke.

Objectives: Malnutrition is an independent risk factor for poor outcomes in patients who suffered an acute ischemic stroke (AIS). The controlling nutritional status (CONUT) score can provide information for nutritional management in AIS patients. However, the risk factors associated with the CONUT score have not been established to date.

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Complex diseases often involve the interplay between genetic and environmental factors. Charcot-Marie-Tooth type 2 neuropathies (CMT2) are a group of genetically heterogeneous disorders, in which similar peripheral neuropathology is inexplicably caused by various mutated genes. Their possible molecular links remain elusive.

Limb heaviness as a sensorimotor disorder alters rehabilitation adherence after a stroke.

Introduction: To the best of our knowledge, it is still unknown how perceived limb heaviness affects rehabilitation adherence. As rehabilitation adherence is very important for the functional recovery of patients with stroke, it is important to explore the relationship between perceived limb heaviness and rehabilitation adherence.

Methods: We retrospectively reviewed the data of patients with consecutive stroke recruited in the CIRCLE study.

Effect of Clamping Compression on the Mechanical Performance of a Carbon Paper Gas Diffusion Layer in Polymer Electrolyte Membrane Fuel Cells.

During all the assembly stages of a polymer electrolyte membrane fuel cell (PEMFC) stack, gas diffusion layers (GDLs) endure clamping loads in the through-plane direction several times. Under such complicated assembly conditions, GDLs have to deform with the changes in structure, surface roughness, pore size, etc. A comprehensive understanding of the compressive performance of GDLs at different clamping phases is crucial to the assembly process improvement of PEMFCs.

Solution-Processed CsPbBr Quantum Dots/Organic Semiconductor Planar Heterojunctions for High-Performance Photodetectors.

Planar heterojunctions (PHJs) are fundamental building blocks for construction of semiconductor devices. However, fabricating PHJs with solution-processable semiconductors such as organic semiconductors (OSCs) is a challenge. Herein, utilizing the orthogonal solubility and good wettability between CsPbBr perovskite quantum dots (PQDs) and OSCs, fabrication of solution-processed PQD/OSC PHJs are reported.

The Effect of Preoperative Rehabilitation Training on the Early Recovery of Joint Function after Artificial Total Knee Arthroplasty and Its Effect Evaluation.

Objective: To study the effect of preoperative rehabilitation training on the rehabilitation of patients after total knee arthroplasty.

Methods: A total of 120 patients diagnosed with knee osteoarthritis and undergoing total knee arthroplasty were selected and divided into experimental group and control group according to a random number table, with 60 cases in each group. The control group only carried out routine clinical nursing before the operation, and the experimental group used the formulated preoperative rehabilitation training method based on the control group for training.

Stroke-associated pneumonia: A bibliometric analysis of worldwide trends from 2003 to 2020.

Stroke-associated pneumonia (SAP) is a spectrum of pulmonary infections in patients within 7 days of stroke. Which is one of the most common complications after stroke and is significantly associated with a poor prognosis of stroke. To the best of our knowledge, a bibliometric method was not previously used to analyze the topic of SAP; we aim to describe the situation and evolution of SAP from 2003 to 2020, and to discuss the research hotspots and frontiers.

Effects of High-Voltage Pulsed Radiofrequency on the Ultrastructure and Nav1.7 Level of the Dorsal Root Ganglion in Rats With Spared Nerve Injury.

Objectives: To investigate the analgesic effect of high-voltage pulsed radiofrequency (HV-PRF) on the dorsal root ganglion (DRG) for neuropathic pain induced by spared nerve injury (SNI) in rats, especially the influence of this treatment on the DRG ultrastructure and voltage-gated sodium channel 1.7 (Nav1.7) level in the DRG.

Hesperetin attenuates silica-induced lung injury by reducing oxidative damage and inflammatory response.

Oxidative stress and the inflammatory response are two important mechanisms of silica-induced lung injury. Hesperetin (HSP) is a natural flavonoid compound that is found in citrus fruits and has been indicated to exhibit strong antioxidant and anti-inflammatory properties. The current study evaluated the protective effect of HSP on lung injury in rats exposed to silica.

Activities of daily living measurement after ischemic stroke: Rasch analysis of the modified Barthel Index.

In patients with ischemic stroke, activities of daily living were used as an outcome indicator, and correct assessment is very important. We sought to examine the reliability and validity of the modified Barthel Index as an evaluation tool of activities of daily living in ischemic stroke patients by applying the Rasch analysis.We used a prospectively collected cohort of ischemic stroke patients in the department of neurology.

A Series of Novel HDAC Inhibitors with Anthraquinone as a Cap Group.

Although anthraquinone derivatives possess significant antitumor activity, most of them also displayed those side effects like cardiotoxicity, mainly owing to their inhibition of topoisomerase II of DNA repair mechanisms. Our raised design strategy by switching therapeutic target from topoisomerase II to histone deacetylase (HDAC) has been applied to the design of anthraquinone derivatives in current study. Consequently, a series of novel HDAC inhibitors with a tricylic diketone of anthraquinone as a cap group have been synthesized.

Design of a high-resolution light field miniscope for volumetric imaging in scattering tissue.

Integrating light field microscopy techniques with existing miniscope architectures has allowed for volumetric imaging of targeted brain regions in freely moving animals. However, the current design of light field miniscopes is limited by non-uniform resolution and long imaging path length. In an effort to overcome these limitations, this paper proposes an optimized Galilean-mode light field miniscope (Gali-MiniLFM), which achieves a more consistent resolution and a significantly shorter imaging path than its conventional counterparts.

Optical-aberrations-corrected light field re-projection for high-quality plenoptic imaging.

The singlet plenoptic camera, which consists of a single lens, microlens array (MLA) and image sensor, possesses the superiority that the imaging system is compact and lightweight, which is beneficial to miniaturization. However, such plenoptic cameras suffer from severe optical aberrations and their imaging quality is inferior for post-capture processing. Therefore, this paper proposes an optical-aberrations-corrected light field re-projection method to obtain high-quality singlet plenoptic imaging.

A Three-Dimensional Finite Element Analysis Model for SH-SAW Torque Sensors.

In this paper, a three-dimensional finite element analysis (3D-FEA) model for shear horizontal surface acoustic wave (SH-SAW) torque sensors is presented. Torque sensors play a significant role in various fields to ensure a reliable torque transmission in drivelines. Featured with the advantages of high propagation velocity, large Q-value, and good power capacity, SH-SAW based torque sensors are promising but very few studies have been carried out.

An Investigation of the Compressive Behavior of Polymer Electrode Membrane Fuel Cell's Gas Diffusion Layers under Different Temperatures.

In this paper, a commercial gas diffusion layer is used, to quantitatively study the correlation between its compressive characteristics and its operating temperature. In polymer electrode membrane fuel cells, the gas diffusion layer plays a vital role in the membrane electrode assembly, over a wide range of operating temperatures. Therefore, understanding the thermo-mechanical performance of gas diffusion layers is crucial to design fuel cells.

Effects of Freeze⁻Thaw Thermal Cycles on the Mechanical Degradation of the Gas Diffusion Layer in Polymer Electrolyte Membrane Fuel Cells.

In this paper, the mechanical degradation of a commercial gas diffusion layer subjected to repeated freeze⁻thaw thermal cycles is studied. In a fuel cell, the mechanical assembly state directly affects the performance of polymer electrolyte membrane fuel cells. Particularly, the gas diffusion layer repeatedly withstands the complex heat and humidity environmental conditions in which the temperature and humidity are always greatly changed.

Point spread function and depth-invariant focal sweep point spread function for plenoptic camera 2.0.

This paper derives a mathematical point spread function (PSF) and a depth-invariant focal sweep point spread function (FSPSF) for plenoptic camera 2.0. Derivation of PSF is based on the Fresnel diffraction equation and image formation analysis of a self-built imaging system which is divided into two sub-systems to reflect the relay imaging properties of plenoptic camera 2.

Birth of a Mosaic Non–Down Syndrome Offspring to a 45,XY,t(21;21)(q10;q10) Homologous Robertsonian Translocation Carrier: A Case Report.

BACKGROUND: Homologous Robertsonian translocation carriers have been considered to have no chance, theoretically, of having a normal embryo. For these carriers, traditional genetic counseling would suggest using donated sperm or oocyte rather than using their own gametes. CASE: This study reports the case of a 45,XY,t(21;21)(q10;q10) homologous Robertsonian translocation carrier who had an approximately normal offspring with a mosaic karyotype: 46,XX,[86]/46, XX,t(21;21)[14].

Distance measurement based on light field geometry and ray tracing.

In this paper, we propose a geometric optical model to measure the distances of object planes in a light field image. The proposed geometric optical model is composed of two sub-models based on ray tracing: object space model and image space model. The two theoretic sub-models are derived on account of on-axis point light sources.

A high-performance nanobridged MoO UV photodetector based on nanojunctions with switching characteristics.

Ultraviolet photodetectors (UVPDs) were fabricated via the in situ growth of orthorhombic MoO nanobelts among interdigital electrodes. Two types of nanojunction, touching and interpenetration, were formed between neighboring nanobelts at different growth conditions. The photoresponse mechanism of the UVPDs greatly depends on the type of nanojunction.