Mechanistic insights into Quetiapine's Protective effects on cognitive function and synaptic plasticity in epileptic rats.

The study aimed to examine the effects of Quetiapine, an atypical antipsychotic medication with purported neuroprotective qualities, on cognitive function and synaptic plasticity in epileptic rats. This investigation also sought to elucidate the mechanisms by which quetiapine influences the activity of the cyclic adenylate response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway and metallomatrix proteinase-9 (MMP9) expression in the context of epilepsy. The epileptic model was induced in rats through the administration of pilocarpine, with normal rats serving as the control group.

The Efficacy for Hypertensive Intracerebral Hemorrhage Between Neuroendoscopic Surgery and Conservative Treatment: A Retrospective Observational Study.

Objectives: This study aims to investigate the efficacy of neuroendoscopic surgery in the treatment of hypertensive intracerebral hemorrhage (HICH).

Methods: A total of 193 patients diagnosed with HICH were divided into 2 groups in this study: the observation group (n=101) received neuroendoscopic surgery, whereas the control group (n=92) underwent conservative treatment. Then, the outcomes between these 2 groups were compared and assessed.

The causal relationship between gut microbiota and 2 neoplasms, malignant and benign neoplasms of bone and articular cartilage: A two-sample Mendelian randomization study.

Previous research has demonstrated a close connection between the development of bone neoplasms and variations in the abundance of specific gut microbiota. It remains unclear, however, how the gut microbiota and bone neoplasms are causally related. Hence, in our study, we aim to clarify this relationship between gut microbiota and 2 neoplasms, malignant neoplasm of bone and articular cartilage (MNBAC) and benign neoplasm of bone and articular cartilage (BNBAC), by employing a two-sample Mendelian randomization (MR) approach.

Metabolome and Transcriptome Combined Reveal the Main Floral Volatile Compounds and Key Regulatory Genes of .

Chestnut () is an economically important forest tree species, and its flowers possess functions such as repelling mosquitoes, killing bacteria, and clearing heat. However, the regulatory mechanisms of floral volatile organic compounds (VOCs) in chestnut are still unclear. This study analyzed the contents of major volatile compounds and related gene expression levels in chestnut flowers during the initial flowering stage (IFS) and full-flowering stage (FFS) using metabolomics and transcription techniques.

Analysis of the functional role and mRNA expression of GABA B R in the nucleus accumbens of cocaine-addicted rats.

Background: Drug addiction is a social and medical problem that must be urgently addressed. The nucleus accumbens (NAc) is closely related to addiction-related learning memory, and γ-aminobutyric acid type B receptor (GABA B R) is a potential target for the treatment of drug addiction. However, the role of GABA B R activity levels in the NAc in cocaine addiction is unclear.

Accurate Detection of Multiple Tumor Mutations in Formalin-Fixed Paraffin-Embedded Tissues by Coupling Sequence Artifacts Elimination and Mutation Enrichment With MeltArray.

Formalin-fixed paraffin-embedded (FFPE) tissues are the primary source of DNA for companion diagnostics (CDx) of cancers. Degradation of FFPE tissue DNA and inherent tumor heterogeneity constitute serious challenges in current CDx assays. To address these limitations, we introduced sequence artifact elimination and mutation enrichment to MeltArray, a highly multiplexed PCR approach, to establish an integrated protocol that provides accuracy, ease of use, and rapidness.

Highly Sensitive Detection of PIK3CA Mutations by Looping-Out Probes-Based Melting Curve Analysis.

PIK3CA mutations have important therapeutic and prognostic implications in various cancer types. However, highly sensitive detection of PIK3CA hotspot mutations in heterogeneous tumor samples remains a challenge in clinical settings. To establish a rapid PCR assay for highly sensitive detection of multiple PIK3CA hotspot mutations.

Landscape Pattern and Ecological Risk Assessment in Guilin Based on Land Use Change.

The land use and ecological risk patterns in Guilin, which is the only innovation demonstration zone under the National Sustainable Development Agenda in China with a focus on the sustainable use of natural resources, have changed significantly as a result of the combined impact of climate change and human activities, thus presenting challenges to the sustainable development of the local area. This research employs an ecological risk assessment model and spatial analysis techniques in order to analyze the spatial correlation between land use and ecological risk, and to evaluate the spatial and temporal evolution characteristics of ecological risk at the overall and county scales in Guilin. The results reveal the following: (1) A total of 1848.

Customizing non-diffracting structured beams.

We demonstrate a universal approach to designing and generating non-diffracting structured light beams with arbitrary shapes. Such light beams can be tailored by predefining suitable spectral phases that match the corresponding beam shapes in the transverse plane. We develop a practical spectral superposition algorithm to discuss the non-diffracting properties and experimentally confirm our numerical results.

Auxin and CmAP1 regulate the reproductive development of axillary buds in Chinese chestnut (Castanea mollissima).

Auxin accumulation upregulates the expression of APETALA1 (CmAP1) and subsequently activates inflorescence primordium development in axillary buds of chestnut. The architecture of fruiting branches is a key determinant of chestnut yield. Normally, axillary buds at the top of mother fruiting branches develop into flowering shoots and bear fruits, and the lower axillary buds develop into vegetative shoots.

Pan-genome analysis of three main Chinese chestnut varieties.

Chinese chestnut ( Blume) is one of the earliest domesticated plants of high nutritional and ecological value, yet mechanisms of underlying its growth and development are poorly understood. Although individual chestnut species differ greatly, the molecular basis of the formation of their characteristic traits remains unknown. Though the draft genomes of chestnut have been previously released, the pan-genome of different variety needs to be studied.

AfCHIL, a Type IV Chalcone Isomerase, Enhances the Biosynthesis of Naringenin in Metabolic Engineering.

Naringenin is an essential precursor for all flavonoids, and effectively promoting naringenin production is crucial in metabolic engineering. The interaction between plant metabolic enzymes ensures metabolic flux. The effect can effectively improve the natural product synthesis of engineering microbial systems.

Clinical outcomes of partial sensory root rhizotomy on patients with recurrence of multiple sclerosing trigeminal neuralgia after percutaneous balloon compression.

Objective: To investigate the clinical outcomes of partial sensory root rhizotomy (PSR) on patients with recurrence of multiple sclerosing trigeminal neuralgia(TN-MS) after percutaneous balloon compression (PBC).

Methods: 21 patients with recurrence of TN-MS after PBC were treated with PSR between January 2012 and July 2018. The visual analogue score (VAS) of participants before and after PBC/PSR were observed, and the postoperative recurrence rate of PBC/PSR were recorded, and the postoperative complications were also followed up.

Landscape Pattern and Ecological Risk Assessment in Guangxi Based on Land Use Change.

Due to ecological environmental fragility and soil erosion in Guangxi, studies of landscape patterns and associated ecological risks are needed to guide sustainable land development and ecologically sensitive land management. This study assesses dynamic spatial and temporal change patterns in land use and ecological risks based on 30 m land-use data, analyzes spatial correlations with ecological risks, and explores natural and socio-economic factor impacts on ecological risks. The results reveal: (1) A rapid and sizeable construction land increase in Guangxi from 2000 to 2018 associated mainly with loss of woodland and grassland.

Periostin promotes the proliferation and metastasis of osteosarcoma by increasing cell survival and activates the PI3K/Akt pathway.

Background: Silencing of the periostin gene (POSTN) can inhibit the biological process of several different cancers, and this inhibition may be related to down-regulation of PI3K/AKT signaling. However, the effect of POSTN on the progression, proliferation, and invasion of osteosarcoma (OS) remain unclear.

Methods: We used the Gene Expression Omnibus (GEO) database to screen datasets on in situ OS and lung metastases to identify core genes and potential pathways.

Swallowtail-type diffraction catastrophe beams.

We demonstrate a universal approach for generating high-order diffraction catastrophe beams, specifically for Swallowtail-type beams (abbreviated as Swallowtail beams), using diffraction catastrophe theory that was defined by potential functions depending on the control and state parameters. The three-dimensional curved caustic surfaces of these Swallowtail catastrophe beams are derived by the potential functions. Such beams are generated by mapping the cross sections of the high-order control parameter space to the corresponding transverse plane.

Abruptly autofocusing circular swallowtail beams.

In this Letter, to the best of our knowledge, we report the first experimental demonstration of a new family of autofocusing beams, circular swallowtail beams (CSBs), based on the high-order swallowtail catastrophe, which were determined by potential functions depending on the state and control parameters. The dynamics of the CSBs is discussed here. These types of CSBs tend to automatically focus without external components.

Hyperplasia suppressor gene inhibits the proliferation and metastasis of glioma cells by targeting rho family proteins.

Aim: To investigate the effect of the hyperplasia suppressor gene (HSG) on human glioma cell invasion and its possible mechanism.

Methods: Human glioma U251 cells were infected with recombinant viral vectors carrying the HSG gene sequence (HSG overexpression group) and HSG interference sequence (HSG suppression group). The negative control group with no-load virus transcription and a blank control group with only PBS treatment were set up.

Chloroplast genomes of seven species of Coryloideae (Betulaceae): structures and comparative analysis.

Coryloideae is a subfamily in the family Betulaceae consisting of four extant genera: , , , and . We sequenced the plastomes of six species of and one species of for comparative and phylogenetic analyses. The plastomes are 159-160 kb long and possess typical quadripartite cp architecture.

Heterologous Expression of from L. Promotes Flowering in L.

Methyl-CpG-binding domain (MBD) proteins have diverse molecular and biological functions in plants. Most studies of MBD proteins in plants have focused on the model plant L. Here we cloned from the willow L.

Generation of spirally accelerating optical beams.

We developed a generalized spectral phase superposition approach for generating accelerating optical beams along arbitrary trajectories. Such beams can be customized by predefining an appropriate superimposed phase pattern that consists of multiple sub-phases. We generated a spirally accelerating beam in a three-dimensional space and developed an algorithm to improve the uniformity of the intensity along the trajectory by introducing phase-shift factors.

Transcriptome Sequencing Analyses between the Cytoplasmic Male Sterile Line and Its Maintainer Line in Welsh Onion (Allium fistulosum L.).

Cytoplasmic male sterility (CMS) is important for exploiting heterosis in crop plants and also serves as a model for investigating nuclear-cytoplasmic interaction. The molecular mechanism of cytoplasmic male sterility and fertility restoration was investigated in several important economic crops but remains poorly understood in the Welsh onion. Therefore, we compared the differences between the CMS line 64-2 and its maintainer line 64-1 using transcriptome sequencing with the aim of determining critical genes and pathways associated with male sterility.

[The clinical analysis of plakophilin-2 gene mutation in patients with arrhythmogenic right ventricular cardiomyopathy/dysplasia].

Objective: The purpose of this study was to screen genetic variations in plakophilin-2 (PKP2) gene in patients with arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) and investigate the differences in clinical features between mutation and no-mutation groups.

Methods: Thirty unrelated Chinese patients clinically diagnosed with ARVC/D and 50 healthy controls were included. Genomic DNA was isolated from peripheral blood samples.

[Effects of GABAB receptor expression level on cognitive impairment and Arc/Arg3.1 expression in induced epileptic rats model].

Objective: To investigate the effects of GABAB receptor on cognitive impairment by using pilocarpine induced kindled rats model and also to check early gene (Arc/Arg3.1) expression.

Methods: Pilocarpine induced kindled rats were divided into four groups (Group normal, Baclofen, CGP and Kindled) randomly, and every group included 20 rats.

Improved long-term survival associated with stent deployment during percutaneous coronary interventions: results from a registry of 3399 patients.

Introduction: The use of stents in percutaneous coronary intervention (PCI) improves procedural success and reduces restenosis. However, few studies have had a sufficient sample size or adequate follow-up to determine whether this advantage results in a positive effect on mortality.

Methods: A total of 3399 patients undergoing PCI (stented [with dual antiplatelet therapy]: n = 2456, nonstented [balloon PCI or rotational atherectomy]: n = 942) at a single institution from 1994 to 2001 were followed up prospectively (43 +/- 22 and 54 +/- 25 months, respectively) for acute and long-term clinical outcomes.