Screening and identification of the active components from Puerariae Radix by HUVEC/CMC-LC-MS.

Puerariae Radix (PR) serves as food and medicinal plant for thousands of years with explicit efficacy for heart diseases, while biological target specifically binding-oriented screening of the active components in PR remains a preliminary stage. Cell membrane chromatography (CMC) is newly developed approach where interactions between active components and certain biological targets can be effectively studied, Human umbilical vein endothelial cell (HUVEC) membrane, with its abundant receptors such as β and AT, is most eligible for constructing CMC. In this study, an HUVEC/CMC-LC-MS system was developed for screening active components in PR, 11 compounds were screened out and four of them were identified.

An efficient and environmental-friendly dispersant based on the synergy of amphiphilic surfactants for oil spill remediation.

Eliminating the adverse environmental impact of chemical dispersants for oil spill has been a significant challenge since decades ago. Here, we prepared an effective and environmentally-friend dispersant with stable emulsifying capacity by the soybean lecithin and Tween 80. The mean droplet diameters of emulsions prepared by decane decreased from 11.