Establishing a semi-homology-directed recombination method for precision gene integration in axolotls.

The axolotl is broadly used in regenerative, developmental, and evolutionary biology research. Targeted gene knock-in is crucial for precision transgenesis, enabling disease modeling, visualization, tracking, and functional manipulation of specific cells or genes of interest (GOIs). Existing CRISPR/Cas9-mediated homology-independent method for gene knock-in often causes "scars/indels" at integration junctions.

Evaluating the impacts of drilling and extraction activities on the marine carbonate system in the natural gas fields of Beibu Gulf, Northern South China Sea.

Natural gas fields are typically located in shallow gulfs. Previous studies have predominantly focused on gas leakage and its subsequent toxic effects on marine organisms; however, the impacts of accidental CO leaks on carbonate dynamics during drilling and extraction remain poorly understood. In this study, we investigate carbonate parameters in two gas fields to elucidate the influences of extraction activities and natural processes on carbonate dynamics in Beibu Gulf, situated in the northern South China Sea (nSCS).

Microplastics affect the nitrogen nutrition status of soybean by altering the nitrogen cycle in the rhizosphere soil.

Microplastics (MPs) are widely distributed in agricultural systems. However, studies on the comprehensive effects of MPs on nitrogen cycling in crop rhizosphere soil, and the changes this effect causes to crop growth is still limited. In this study, we investigated how three types of 5 % MPs (polystyrene, PS; polyethylene, PE; polyvinyl chloride, PVC) affect soybean growth by altering rhizosphere soil nitrogen cycling.

Serum Metabolomic Analysis of Healthy and Central Precocious Puberty Girls.

Background: The incidence of precocious puberty (PP) has been on the rise in recent years. Based on different control mechanisms, childhood PP is divided into central precocious puberty (CPP) and peripheral precocious puberty (PPP). CPP accounts for 80% of all PP cases.

Sequence Context-Agnostic TadA-Derived Cytosine Base Editors for Genome-Wide Editing in Zebrafish.

Single-nucleotide variants (SNVs) represent a significant form of genetic variation linked to various diseases. CRISPR-mediated base editing has emerged as a powerful method for modeling diseases caused by SNVs, particularly in zebrafish, which serve as an excellent platform for investigating disease mechanisms and conducting drug screenings. However, existing cytosine base editors (CBEs) for zebrafish often have broad editing windows and strong sequence preferences, limiting their effectiveness.

Revealing the biological features of the axolotl pancreas as a new research model.

Introduction: The pancreas plays a crucial role in digestion and blood glucose regulation. Current animal models, primarily mice and zebrafish, have limited the exploration of pancreatic biology from an evolutionary-developmental perspective. Tetrapod vertebrate axolotl () serves as a valuable model in developmental, regenerative, and evolutionary biology.

Improving Spatial Transcriptomics with Membrane-Based Boundary Definition and Enhanced Single-Cell Resolution.

Accurately defining cell boundaries for spatial transcriptomics is technically challenging. The current major approaches are nuclear staining or mathematical inference, which either exclude the cytoplasm or determine a hypothetical boundary. Here, a new method is introduced for defining cell boundaries: labeling cell membranes using genetically coded fluorescent proteins, which allows precise indexing of sequencing spots and transcripts within cells on sections.

Robust fluorinated cellulose composite aerogels incorporating radiative cooling and thermal insulation for regionally adaptable building thermal management.

Passive radiative cooling (PRC) is an emerging sustainable technology that plays a key role for achieving the goal of carbon neutrality. However, several challenges remain for PRC materials in their practical application in building thermal management, including overcooling problems and unsatisfactory cooling efficiency caused by solar absorption and parasitic heat gains. In this work, fluorinated cellulose-based composite aerogels (FCCA) integrating thermal insulation and PRC were developed by a facile manufacturing strategy that combined phase separation and freeze-drying.

Surgical interventions for spontaneous supratentorial intracerebral haemorrhage: a systematic review and network meta-analysis.

Background: Surgical interventions for spontaneous supratentorial intracerebral haemorrhage (ICH) include conventional craniotomy (CC), decompressive craniectomy (DC), and minimally invasive surgery (MIS), with the latter encompassing endoscopic surgery (ES) and minimally invasive puncture surgery (MIPS). However, the superiority of surgery over conservative medical treatment (CMT) and the comparative benefits of different surgical procedures remain unclear. We aimed to evaluate the efficacy and safety of various surgical interventions for treating ICH.

Triple-mode sensing platform for acetylcholinesterase activity monitoring and anti-Alzheimer's drug screening based on a highly stable Cu (I) compound.

Acetylcholinesterase (AChE) and AChE inhibitors play critical roles in the early diagnosis and treatment of Alzheimer's disease (AD). Herein, a fluorescence/colorimetry/smartphone triple-mode sensing platform was constructed for both AChE activity monitoring and AChE inhibitor screening by exploring a Cu (I) compound, CuI (SR) (R = CHCHNH), as a fluorescent probe. In comparison of most other fluorescent probes, CuI (SR) presented exceptional stability against pH, temperature, UV irradiation, redox agents, and metal ions, as well as good recyclability due to its unique chemical structure.

Correlation of systemic immune inflammation and serum uric acid with gout: based on NHANES.

Objectives: This study aimed to explore the relationship of systemic immune inflammation index (SII) and uric acid with gout using NHANES 2017-2018 data.

Method: Data were collected from the 2017-2018 circle of the NHANES database. SII was calculated based on the counts of lymphocytes (LC), neutrophils (NC), and platelets (PC).

Mass balance, metabolism, and pharmacokinetics of [C]amdizalisib, a clinical-stage novel oral selective PI3Kδ inhibitor for the treatment of non-hodgkin's lymphoma, in healthy Chinese volunteers.

Introduction: Amdizalisib (HMPL-689) is an ATP-competitive PI3Kδ inhibitor currently under investigation for treating Hodgkin's lymphoma. This study aimed to evaluate the metabolism, excretion, pharmacokinetics, and safety profile of amdizalisib in healthy human subjects to support its clinical application.

Methods: This Phase I clinical trial included six healthy Chinese male volunteers who received a single oral dose of 30 mg/100 µCi [C]amdizalisib suspension.

Spectrally selective and thermally insulating hybrid nanofiber aerogel coolers for building energy conservation.

With the advancement of worldwide carbon neutralization, passive radiative cooling (PRC) has attracted tremendous interest in energy conservation by dissipating heat into the ultracold outer space without any electricity consumption. Despite some progress has been made in tailoring spectral properties for PRC, it still remains a crucial challenge in fabricating efficient and low-cost coolers for building energy saving. Herein, hierarchical cellulose-based aerogel coolers consisting of beads-on-string structural electrospun nanofibers are manufactured for all-day and all-region energy saving by combining radiative cooling and thermal insulation in one design.

Cytosine base editors with increased PAM and deaminase motif flexibility for gene editing in zebrafish.

Cytosine base editing is a powerful tool for making precise single nucleotide changes in cells and model organisms like zebrafish, which are valuable for studying human diseases. However, current base editors struggle to edit cytosines in certain DNA contexts, particularly those with GC and CC pairs, limiting their use in modelling disease-related mutations. Here we show the development of zevoCDA1, an optimized cytosine base editor for zebrafish that improves editing efficiency across various DNA contexts and reduces restrictions imposed by the protospacer adjacent motif.

Risk of angioedema and thrombolytic therapy among stroke patients: An analysis of data from the FDA Adverse Event Reporting System database.

The angioedema risk may vary among stroke patients receiving different thrombolytic agents. This study aimed to investigate the angioedema risk associated with different thrombolytic agents and to identify associated risk factors. We conducted a large-scale retrospective pharmacovigilance study using the FDA Adverse Event Reporting System (FAERS) database.

[Correlation between triglyceride glucose product index and food intake and type 2 diabetes].

Objective: To explore the relationship between triglyceride-glucose index(triglyceride-glucose index) and food intake and the comprehensive compliance rate in patients with type 2 diabetes mellitus(T2DM).

Methods: A total of 6683 T2DM patients aged 18 to 75 years entered the National Metabolic Management Center(MMC) in two Yancheng hospitals from August 2020 to November 2023 were selected to analyze the relationship between T2DM standard attainment rate and relevant indicators. Univariate analysis was conducted with χ~2 test, multivariate analysis was conducted with stepwise logistic regression model, and the relationship of TyG to the overall compliance rate of T2DM was analyzed with restricted cubic spline(Restricted Cubic Spline, RCS).

Nine months of bedaquiline, linezolid, levofloxacin, clofazimine, and cycloserine chemotherapy for rifampicin/multidrug-resistant tuberculosis: a multicenter, randomized, open-label non-inferiority trial in China.

Background: We concurrently developed a prospective study to assess clinical outcomes among patients receiving 9-month bedaquiline (BDQ)-containing regimens, aiming to provide valuable data on the use of this short-course regimen in China.

Methods: This open-label, randomized, controlled, multicenter, non-inferiority trial was conducted at sixteen hospitals, and enrolled participants aged 18 years and older with pulmonary rifampicin/multidrug tuberculosis. Participants were randomly assigned, in a 1:1 ratio.

Value of Glycemic Dispersion Index in Predicting Major Adverse Cardiovascular Events in Diabetic Patients with Concomitant Acute Coronary Syndrome.

Aim: This investigation aims to assess the predictive value of the glycemic dispersion index (GDI), calculated by incorporating glycated hemoglobin, fasting plasma glucose, and 2-hour postprandial plasma glucose, in predicting major adverse cardiovascular events (MACE) within a 12-month timeframe for diabetic patients with concomitant acute coronary syndrome (ACS).

Methods: A retrospective study was conducted on 3261 diabetic patients with ACS who were hospitalized in the Department of Cardiology, the Sixth Affiliated Hospital of Kunming Medical University, from January 2016 to July 2022. Based on the inclusion and exclusion criteria, 512 patients were ultimately enrolled in the study.

The Dynamics of Methylation Concentrations in Glutathione Peroxidase 3 Promoter from Patients with Chronic Heart Failure and Their Association with Key Clinical Parameters.

Objective: This study investigated changes in methylation concentrations within the glutathione peroxidase 3 (GPX3) promoter region among patients diagnosed with chronic heart failure (CHF). Peripheral blood samples were collected from 20 CHF patients and 20 healthy individuals for analysis.

Methods: Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, methylation concentrations of 11 CpG sites within the GPX3 promoter region were quantified.

Correction: Liu et al. Behaviour Is Influenced by Volatiles from Both Plants and Nymphs. 2019, , 141.

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Occurrence of Maize Leaf Spot Disease Caused by in Yunnan, China.

Maize (Zea mays) is vital as a staple food and livestock feed crop. Yunnan is one of the main maize-producing provinces in China (National Bureau of Statistics, 2022). While corn production in Yunnan is lower than the national average, the development of drought-tolerant varieties has contributed to improving productivity.