Identification and Functional Analysis of the Gene Conferring Resistance to Late Blight () in Tomato.

Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene confers resistance against the race T of in tomatoes.

Dissection of major QTLs and candidate genes for seedling stage salt/drought tolerance in tomato.

Background: As two of the most impactful abiotic stresses, salt and drought strongly affect tomato growth and development, especially at the seedling stage. However, dissection of the genetic basis underlying salt/drought tolerance at seedling stage in tomato remains limited in scope.

Results: Here, we reported an analysis of major quantitative trait locus (QTL) and potential causal genetic variations in seedling stage salt/drought tolerance in recombinant inbred lines (n = 201) of S.

Comprehensive screening of industrially relevant components at genome scale using a high-quality gene overexpression collection of Corynebacterium glutamicum.

Development of efficient microbial strains for biomanufacturing requires deep understanding of the biology and functional components responsible for the synthesis, transport, and tolerance of the target compounds. A high-quality controllable gene overexpression strain collection was constructed for the industrial workhorse Corynebacterium glutamicum covering 99.7% of its genes.

Accumulation characteristics of plant flavonoids and effects of cultivation measures on their biosynthesis: A review.

Flavonoids, a kind of secondary metabolites with both edible, medicinal and antioxidant purposes, could be widely used in food, drug processing, forest products, chemical industry and many other fields. Flavonoid production in plant organs were influenced by numerous internal and external factors at various stages, leading to differential gene expression and transcription factors activity. This study reviews the characteristics of major flavonoids categories, their distribution and accumulation in different plant parts and analyzing their molecular mechanisms.

Candidate Gene Identification and Transcriptome Analysis of Tomato - and Functional Marker Development for - and Its Alleles, -, -, and -.

Male sterility is a valuable trait for hybrid seed production in tomato (). The mutants - (-) and - of tomato exhibit twisted stamens, exposed stigmas, and complete male sterility, thus holding potential for application in hybrid seed production. In this study, the - and - loci were fine-mapped to 53.

Additive efficacy and safety of probiotics in the treatment of ulcerative colitis: a systematic review and meta-analysis.

Background: We aim to report the latest pooled analyses to evaluate the additive efficacy and safety of probiotics in the treatment of ulcerative colitis (UC).

Methods: We systematically searched the relevant literature investigating the efficacy and/or safety of probiotics in patients with UC from PubMed, Embase and Web of Science up to January 2023. Two researchers independently screened the literature, extracted data, and evaluated the quality of the included studies according to the inclusion and exclusion criteria.

Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids.

Biosensors are powerful tools for detecting, real-time imaging, and quantifying molecules, but rapidly constructing diverse genetically encoded biosensors remains challenging. Here, we report a method to rapidly convert enzymes into genetically encoded circularly permuted fluorescent protein-based indicators to detect organic acids (GECFINDER). ANL superfamily enzymes undergo hinge-mediated ligand-coupling domain movement during catalysis.

Fine Mapping of , a Major-Effect Quantitative Trait Locus That Controls Fruit Weight in Tomato.

Tomato () is a widely consumed vegetable, and the tomato fruit weight is a key yield component. Many quantitative trait loci (QTLs) controlling tomato fruit weight have been identified, and six of them have been fine-mapped and cloned. Here, four loci controlling tomato fruit weight were identified in an F population through QTL seq.

Modulating Sensitivity of an Erythromycin Biosensor for Precise High-Throughput Screening of Strains with Different Characteristics.

Genetically encoded biosensors are powerful tools for product-driven high-throughput screening in synthetic biology and metabolic engineering. However, most biosensors can only properly function in a limited concentration cutoff, and the incompatible performance characteristics of biosensors will lead to false positives or failure in screening. The transcription factor (TF)-based biosensors are usually organized in modular architecture and function in a regulator-depended manner, whose performance properties can be fine-tuned by modifying the expression level of the TF.

The safety and efficacy of sequential intramuscular/oral ziprasidone treatment of acute episode in patients with schizophrenia: a multicenter, open-labeled study.

Background: Ziprasidone mesylate injection is an atypical antipsychotic drug which is recently approved in China. In combination with its oral formulation, sequential therapy with ziprasidone brings new interventions to patients with agitation in the acute phase of schizophrenia. The purpose of this 7-day multicenter study conducted in China was to evaluate the efficacy and safety of ziprasidone sequential treatment through intramuscular/oral routes in agitated patients with schizophrenia.

Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in .

Genome-scale functional screening accelerates comprehensive assessment of gene function in cells. Here, we have established a genome-scale loss-of-function screening strategy that combined a cytosine base editor with approximately 12,000 parallel sgRNAs targeting 98.1% of total genes in ATCC 13032.

In-Situ Anchoring Pb-Free Cs Bi Br @BiOBr Quantum Dots on NH -Rich Silica with Enhanced Blue Emission and Satisfactory Stability for Photocatalytic Toluene Oxidation.

All-inorganic metal halide perovskite quantum dots (QDs) have attracted attention from researchers with their fascinating optoelectronic properties. However, blue-emitting perovskite QDs typically have low photoluminescence quantum yield (PLQY). For potential commercial applications, it is preferable to replace Pb with an element having low toxicity.

Reconstruction of a Genome-Scale Metabolic Network for MR-1 and Analysis of its Metabolic Potential for Bioelectrochemical Systems.

Bioelectrochemical systems (BESs) based on MR-1 offer great promise for sustainable energy/chemical production, but the low rate of electron generation remains a crucial bottleneck preventing their industrial application. Here, we reconstructed a genome-scale metabolic model of MR-1 to provide a strong theoretical basis for novel BES applications. The model iLJ1162, comprising 1,162 genes, 1,818 metabolites and 2,084 reactions, accurately predicted cellular growth using a variety of substrates with 86.

Preparation of Cellulose Nanocrystals from Jujube Cores by Fractional Purification.

Jujube cores are fiber-rich industrial waste. Dewaxing, alkali treatment, bleaching, and sulfuric acid hydrolysis were used to generate cellulose nanocrystals (CNCs) from the jujube cores in this study. The morphological, structural, crystallinity, and thermal properties of the fibers were investigated using FE-SEM, TEM, AFM, FT-IR, XRD, and TGA under various processes.

Epidemiology of Cardiopulmonary Arrest and Outcome of Resuscitation in PICU Across China: A Prospective Multicenter Cohort Study.

Objective: To investigate the epidemiology and the effectiveness of resuscitation from cardiopulmonary arrest (CPA) among critically ill children and adolescents during pediatric intensive care unit (PICU) stay across China.

Methods: A prospective multicenter study was conducted in 11 PICUs in tertiary hospitals. Consecutively hospitalized critically ill children, from 29-day old to 18-year old, who had suffered from CPA and required cardiopulmonary resuscitation (CPR) in the PICU were enrolled (December 2017-October 2018).

Reconstruction and analysis of genome-scale metabolic model for thermophilic fungus Myceliophthora thermophila.

Myceliophthora thermophila, a thermophilic fungus that can degrade and utilize all major polysaccharides in plant biomass, has great potential in biotechnological industries. Here, the first manually curated genome-scale metabolic model iDL1450 for M. thermophila was reconstructed using an autogenerating pipeline with thorough manual curation.

Heart rate variability and heart rate monitoring of nurses using PPG and ECG signals during working condition: A pilot study.

The use of wearable photoplethysmography (PPG) technology for estimating heart rate (HR) and HR variability (HRV) in the health care system is gaining attention in recent years. However, the performance of these devices remains questionable in their ability to collect data in real working conditions for long-term monitoring. The present study aimed to examine the data collected from nurses during working hours by PPG and electrocardiography (ECG) devices.

An efficient multi-enzyme cascade platform based on mesoporous metal-organic frameworks for the detection of organophosphorus and glucose.

An efficient colorimetric detection platform based on multi-enzyme cascade has been developed for detection of organophosphorus. Firstly, the dual-enzyme platform was prepared and applied for sensitive glucose detection (detection limit 0.32 μM).

CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an L-proline exporter for L-proline hyperproduction.

Development of hyperproducing strains is important for biomanufacturing of biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and discover functional components. Herein, we optimize and use the CRISPR-assisted editing and CRISPRi screening methods to convert a wild-type Corynebacterium glutamicum to a hyperproducer of L-proline, an amino acid with medicine, feed, and food applications. To facilitate L-proline production, feedback-deregulated variants of key biosynthetic enzyme γ-glutamyl kinase are screened using CRISPR-assisted single-stranded DNA recombineering.

Adjunctive vagus nerve stimulation for treatment-resistant depression: a preliminary study.

Background: This study is the first to assess the safety and therapeutic efficacy of vagus nerve stimulation (VNS) as an adjunctive treatment for Chinese patients suffering from treatment-resistant depression (TRD).

Methods: A total of seven patients with TRD underwent surgical implantation of a VNS device were followed over a 9-month period. The 24-item Hamilton Rating Scale for Depression (HAMD-24) and the 14-item Hamilton Anxiety Scale (HAMA) were used to assess depressive and anxiety symptoms, respectively.

Infection of the Asian gray shrew Crocidura attenuata (Insectivora: Soricidae) with Sarcocystis attenuati n. sp. (Apicomplexa: Sarcocystidae) in China.

Background: Data on the genus Sarcocystis in insectivores are limited. The Asian gray shrew Crocidura attenuata is one of the most common species of the insectivore family Soricidae in South Asia and Southeast Asia. To our knowledge, species of Sarcocystis have never been recorded previously in this host.

Lead-Free Cs AgSbCl Double Perovskite Nanocrystals for Effective Visible-Light Photocatalytic C-C Coupling Reactions.

Lead halide perovskite nanocrystals (NCs) have been regarded as a promising potential photocatalyst, owing to their high molar extinction coefficient, low economic cost, adjustable light absorption range, and ample surface active sites. However, the toxicity of lead and its inherent instability in water and polar solvents could hinder their wide application in the field of photocatalysis. Herein, with α-alkylation of aldehydes as a model reaction, C-C bond-forming is demonstrated in high yield by using lead-free double perovskite Cs AgSbCl NCs under visible light irradiation.

Amorphous NiCoB-coupled MAPbI for efficient photocatalytic hydrogen evolution.

It was thought that the organic-inorganic hybrid perovskite MAPbI could be used to collect visible light for the photocatalytic hydrogen evolution reaction (HER). However, its ability to generate H is limited. Herein, we synthesized amorphous NiCoB through a redox method and coupled it with MAPbI to form the NiCoB/MAPbI composite photocatalyst by electrostatic self-assembly.

OBV (obscure vein), a CH zinc finger transcription factor, positively regulates chloroplast development and bundle sheath extension formation in tomato (Solanum lycopersicum) leaf veins.

Leaf veins play an important role in plant growth and development, and the bundle sheath (BS) is believed to greatly improve the photosynthetic efficiency of C plants. The OBV mutation in tomato (Solanum lycopersicum) results in dark veins and has been used widely in processing tomato varieties. However, physiological performance has difficulty explaining fitness in production.