Distribution Characteristics of Microplastics in Surface Seawater off the Yangtze River Estuary Section and Analysis of Ecological Risk Assessment.

Microplastics are widespread in the oceans as a new type of pollutant. Due to the special geographical environment characteristics, the Yangtze River estuary region become hotspot for microplastics research. In 2017 and 2019, surface seawater microplastics samples were collected from five stations off the Yangtze River estuary during four seasons (spring, summer, autumn, and winter).

Study on the Effectiveness and Value of Evidence-Based Nursing and Predictive Nursing in Emergency Treatment of Upper Gastrointestinal Bleeding.

Objective: To study the effectiveness and value of evidence-based nursing and predictive nursing in emergency treatment of upper gastrointestinal bleeding.

Methods: A total of 100 patients with upper gastrointestinal bleeding in our hospital were selected. The period was from January 2020 to June 2022.

Cultivation and biogeochemical analyses reveal insights into biomineralization caused by piezotolerant iron-reducing bacteria from petroleum reservoirs and their application in MEOR.

Interactions between minerals and iron-reducing bacteria under in-situ pressure and temperature conditions play important roles in oil extraction, residual oil methanation, and CO storage in petroleum reservoirs. However, the impacts of pressure on dissimilatory iron-reducing bacteria (DIRB) are poorly understood. Herein, the interactions between clay minerals and microbes under elevated hydrostatic pressure conditions were elucidated through enrichment experiments.

Effects of green tide on microbial communities in waters of the Jiangsu coastal area, China.

Recently, green tide outbreaks have resulted in severe coastal ecology and economic effects in China. Jiangsu coastal areas are usually the site of early green tide outbreaks. To clarify the effects of green tide outbreaks in Jiangsu coastal areas, this study analyzed microbial communities during green tide-free and green tide outbreak periods (May and July, respectively) through 16S rDNA sequencing.

Culture-dependent and culture-independent methods reveal microbe-clay mineral interactions by dissimilatory iron-reducing bacteria in an integral oilfield.

Fe(III) may be reasonably considered as one of the most important electron acceptors in petroleum reservoir ecosystems. The microbial mineralization of clay minerals, especially montmorillonite, is also of great significance to the exploration of petroleum and gas reservoirs. The bioreduction mechanisms of iron-poor minerals in petroleum reservoirs have been poorly investigated.

Optimization and characterization of biosurfactant produced by indigenous isolated from a low permeability reservoir for application in MEOR.

Biosurfactants are expected to be a key factor for microbial enhanced oil recovery (MEOR). In this study, we described the novel biosurfactant-producing strain YZ-2 isolated from a low permeability oil reservoir. We purified and characterized the biosurfactants produced by this YZ-2 strain thin-layer chromatography and MALDI-TOF-MS, revealing them to be fengycins.

Investigation on Plugging and Profile Control of Polymer Microspheres as a Displacement Fluid in Enhanced Oil Recovery.

Polymer microspheres (PMs) are used as a new material to recover residual oil left in unswept oil areas after secondary recovery methods. The fact that the PMs plug the macropores causes the flow direction of the injection fluid to be transferred from macropores to micropores. In order to investigate the plugging and profile control mechanisms of PMs in reservoirs, four kinds of PMs with different particle sizes and four kinds of artificial cores with different permeability were selected for flooding tests, including plugging experiments and profile control experiments.

Characterization of the complete mitochondrial genome of with phylogenetic analysis.

is a dominant species in the Yangtze River estuary. In this study, we first determined the complete mitochondrial genome (mitogenome) of . The mitogenome is 16,143 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and one non-coding control region.

The complete mitochondrial genome of and its phylogenetic implication.

In this study, the complete mitochondrial genome (mitogenome) of was amplified and analyzed. The mitogenome is 16,062 bp in length, encoding the standard set of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and one control region. The nucleotide frequency of the mitogenome is as follows: A, 34.

The complete mitochondrial genome of .

In this study, the complete mitochondrial genome (mitogenome) of was amplified and analyzed. The mitogenome is 15,718 bp in length, encoding 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a control region (CR). The nucleotide frequency of the mitogenome is as follows: A, 34.

Characterization of the complete mitochondrial genome sequence of and phylogenetic analysis.

In this study, the complete mitogenome of was sequenced and annotated. The mitogenome is 15,441 bp in length, containing 37 classical eukaryotic mitochondrial regions (13 typical protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes) and a non-coding control region. Most of the genes are initiated with ATA, ATG, and ATT, though GTG is also used as an initiation codon.

Reconstruction of a digital core containing clay minerals based on a clustering algorithm.

It is difficult to obtain a core sample and information for digital core reconstruction of mature sandstone reservoirs around the world, especially for an unconsolidated sandstone reservoir. Meanwhile, reconstruction and division of clay minerals play a vital role in the reconstruction of the digital cores, although the two-dimensional data-based reconstruction methods are specifically applicable as the microstructure reservoir simulation methods for the sandstone reservoir. However, reconstruction of clay minerals is still challenging from a research viewpoint for the better reconstruction of various clay minerals in the digital cores.

TNF-α Regulates Mast Cell Functions by Inhibiting Cell Degranulation.

Background/aims: The aim of this study was to investigate the involvement of inducible co-stimulatory ligand (ICOSL) expression in stimulation of mast cells (MCs) by TNF-α and the ability of TNF-α stimulation of MCs to influence CD4+ T cell differentiation and function. The mechanisms underlying TNF-α stimulation of MCs were also explored.

Methods: Mast cells and CD4+ T cells were prepared from C57BL/6 mice (aged 6-8 weeks).

Permeability recovery of damaged water sensitive core using ultrasonic waves.

It is imperative to recover the well productivity lose due to formation damage nearby wellbore during variant well operations. Some indispensable issues in conventional techniques make ultrasonic technology more attractive due to simple, reliable, favorable, cost-effective, and environment friendly nature. This study proposes the independent and combined use of ultrasonic waves and chemical agents for the treatment of already damaged core samples caused by exposure to distilled water.

Multiplexed DNA detection using a gold nanorod-based fluorescence resonance energy transfer technique.

A fluorescence resonance energy transfer method for multiplex detection DNA based on gold nanorods had been successfully constructed. This method is simple, easy to operate, good selectivity, no requirement to label the probe molecule and can analyze simultaneously multiple targets of DNA in one sample. The limit of detection for the 18-mer, 27-mer and 30-mer targets is 0.

A K(+)-mediated G-quadruplex formation enhancement fluorescence polarization system based on quantum dots for detection of Hg2+ and biothiols.

A fluorescence polarization homogenous system based on CdTe/CdS QDs that employed a K(+)-mediated G-quadruplex as an enhancer was identified for sensitive and selective detection of Hg(2+) and biothiols in complex samples.

DNAzyme self-assembled gold nanorods-based FRET or polarization assay for ultrasensitive and selective detection of copper(II) ion.

In the paper, we have constructed a very simple, sensitive and promising assay for fluorescence biosensor detection of Cu(2+) in aqueous solutions based on FRET between gold nanorods (AuNRs) and the 3'-TAMRA-labeled substrate strand (Sub) of DNAzyme. The fluorescence of the Sub is quenched when the substrate strand-DNAzyme strand (Sub-Enz) duplex is adsorbed on AuNRs surface through electrostatic interaction. In the presence of Cu(2+), the fluorescence is restored due to the decrease of FRET efficiency caused by the specific cleavage of the Sub by the DNAzyme (Enz), which weakens the electrostatic interaction between the AuNRs and short 3'-TAMRA-labeled DNA fragment.

[Biomass and carbon storage of Phragmites australis and Spartina alterniflora in Jiuduan Shoal Wetland of Yangtze Estuary, East China].

By the methods of field survey and laboratory analysis, an investigation was conducted on the seasonal dynamics of biomass and carbon storage of Phragmites australis and Spartina alterniflora dominated vegetation belts in the Jiuduan Shoal Wetland of Yangtze Estuary, East China in 2010-2012. The organic carbon storage of the biomass (including aboveground part, underground part, and standing litter) of the two plants was the highest in autumn and the lowest in spring. The average carbon storage of the biomass of S.

An ultrasensitive quantum dots fluorescent polarization immunoassay based on the antibody modified Au nanoparticles amplifying for the detection of adenosine triphosphate.

In this work, an ultrasensitive fluorescent polarization immunoassay (FPIA) method based on the quantum dot/aptamer/antibody/gold nanoparticles ensemble has been developed for the detection of adenosine triphosphate (ATP). DNA hybridization is formed when ATP is present in the PBS solution containing the DNA-conjugated quantum dots (QDs) and antibody-AuNPs. The substantial sensitivity improvement of the antibody-AuNPs-enhanced method is mainly attributed to the slower rotation of fluorescent unit when QDs-labeled oligonucleotides hybridize with antibody modified the gold nanoparticle.

Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.

We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.

[Contribution rates of landscape driving factors in coastal reclamation zone based on CLUE-S model validation].

Based on the TM images, social-economic data, and field investigation data in 1990, 2000, and 2009, and by adopting redundancy analysis (RDA) and principal component analysis (PCA), this paper analyzed the contribution rates of landscape driving forces in coastal area of Fengxian reclamation zone at Yangtze Estuary. Kappa index was used to validate the simulation effectiveness of CLUE-S model. In the study area, anthropogenic landscape driving factors had a higher contribution rate than natural landscape driving factors (57.

[Ecological benefits of greening and related controlling factors in urban residential areas of Hangzhou: a quantitative analysis].

Based on the 1 m x 1 m high resolution aerial images in 2007 and the 30 m x 30 m Landsat 5 TM images in summer 2007, and with the help of GIS and remote sensing image interpretation, this paper calculated the normalized difference vegetation indices (NDVI) representing the overall ecological benefits of greening as well as the six controlling factors, i.e., multilayer structure height, area ratio of softness to hardness, greening rate, floor area ratio, greening area, and building density, in 30 typical urban residential quarters of west Hangzhou.

[Spatiotemporal dynamics of land use and land cover changes in different reclamation zones of Yangtze Estuary].

Based on the TM and ETM remotely sensed images of Yangtze Estuary in 1987, 1995, 2000, and 2006, and by using RS, GIS, and mathematical statistical methods, this paper analyzed the dynamic changes of land use and land cover in different reclamation zones of the estuary. In terms of relative area occupied by each land use type, grassland showed significant spatial differences (P < 0.05) among the reclamation zones, while greenhouse land and paddy field showed significant temporal differences (P < 0.

Effects of light on the growth and clonal reproduction of Ligularia virgaurea.

Ligularia virgaurea is a perennial herb that is widely distributed in the alpine meadow on the eastern Qinghai-Tibet plateau. We investigated the patterns of growth and reproduction of L. virgaurea under two contrasting levels of light conditions for two continuous growing seasons.