Evaluating the role of high NO affinity complete denitrifiers and non-denitrifying NO reducing bacteria in reducing NO emissions in river.

Freshwater rivers are hotspots of NO greenhouse gas emissions. Dissolved organic carbon (DOC) is the dominant electron donor for microbial NO reduction, which can reduce NO emission through enriching high NO affinity denitrifiers or enriching non-denitrifying NO-reducing bacteria (NORB), but the primary regulatory pathway remains unclear. Here, field study indicated that high DOC concentration in rivers enhanced denitrification rate but reduced NO flux by improving nosZ gene abundance.

Detecting serologically difficult ABO blood groups using single-molecule real-time sequencing technology.

Background And Objectives: Recently, third-generation long-read sequencing technology has been increasingly applied to the detection of various blood group systems. Because of its long read length and use of single-molecule sequencing, it is capable of obtaining the sequences of blood group genes in their entirety as well as of distinguishing haplotypes. Therefore, here, we collected ABO blood group samples that were difficult to classify serologically and analysed the sequences of the coding regions of the ABO genes as well as the sequences upstream and downstream of the coding regions.

A-site defective LaCuO perovskite-type oxides for efficient oxidation of cyclohexylbenzene.

Phenol production through the oxidation of cyclohexylbenzene (CHB) and the subsequent decomposition of tertiary hydroperoxide has attracted more and more attention. In this study, defective LaCuO perovskite-type oxide catalysts with tunable A-site deficient structures and abundant surface oxygen vacancies were developed for the liquid phase oxidation of CHB to produce cyclohexylbenzene-1-hydroperoxide (CHBHP). By tuning the amount of A-site La ions in the perovskite structure, more surface oxygen vacancies and Cu species were formed in catalysts.

Serum metabolomic and lipidomic profiling identifies diagnostic biomarkers for seropositive and seronegative rheumatoid arthritis patients.

Background: Diagnosing seronegative rheumatoid arthritis (RA) can be challenging due to complex diagnostic criteria. We sought to discover diagnostic biomarkers for seronegative RA cases by studying metabolomic and lipidomic changes in RA patient serum.

Methods: We performed comprehensive metabolomic and lipidomic profiling in serum of 225 RA patients and 100 normal controls.

Oxysterol-binding protein-related protein 4L promotes cell proliferation by sustaining intracellular Ca2+ homeostasis in cervical carcinoma cell lines.

Oxsterol binding protein-related protein 4 (ORP4) is essential for cell proliferation, but the underlying mechanism is unclear. ORP4 is expressed as three variants, ORP4L, ORP4M and ORP4S. Here, we reported that silencing of ORP4L with specific small interfering RNA (siRNA) inhibited the proliferation of human cervical cancer cell lines C33A, HeLa and CaSki, the reverse effect being observed in ORP4L overexpressing cells.