Characterization of a stable QTL for quality-related traits and its effects on yields in wheat (Triticum aestivum L.).

A total of 6 major stable QTLs and 59 pairwise epistatic eQTLs for quality-related traits were identified, and the candidate genes underlying qDt-KJ 4B, a novel major and stable QTL for dough tractility, were identified Wheat quality traits are usually negatively correlated with yield traits, but they affect the processing quality and nutritional value of wheat. Therefore, identifying more wheat quantitative trait loci (QTLs) and elucidating their genetic basis are essential for cultivating new high-quality and high-yielding wheat varieties. In this study, QTL analysis for five quality-related traits was performed on a recombinant inbred line (RIL) mapping population, KJ-RIL, derived from a cross between Kenong 9204 (KN9204) and Jing 411 (J411).

The protective effects and mechanism of myricetin in liver diseases (Review).

Liver diseases have become one of the significant threats to global health. However, there is a lack of effective targeted therapeutic drugs in this field and the existing drugs used for liver disease treatment usually have side‑effects. Traditional Chinese medicine (TCM) has the distinctive advantages of multi‑target and low side‑effects.

The Novel HLA-DQB1*02:01:01:27 Allele Identified by HLA-Targeted HiFi Sequencing in a Chinese Individual.

HLA-DQB1*02:01:01:27 differs from HLA-DQB1*02:01:01:01 by the deletion of a single Thymine nucleotide in intron 2.

Ecological risk assessment of future suitable areas for under the background of climate change.

Introduction: is a well-known medicinal plant that belongs to woody liana, possessing high therapeutic and economic value. The market demand of is huge, but its wild resources are scarce and artificial cultivation methods have not been established, which leads to a situation with strong contradiction and imbalance between supply and demand.

Methods: In this study, 303 sample of distribution data for in China were collected, 33 environmental variables related to terrain, climate and soil were analyzed and the suitable habitats of during various periods were predicted by MaxEnt model and ArcGIS software, aiming to provide a basis for scientific cultivation and effective utilization of resources.

Nicotinamide n-methyltransferase inhibitor synergizes with sodium-glucose cotransporter 2 inhibitor to protect renal tubular epithelium in experimental models of type 2 diabetes mellitus.

Aims: We aim to explore the potential of nicotinamide n-methyltransferase (NNMT) as a sensitive marker of renal tubular injury and the possibility of an NNMT inhibitor to combine with sodium-glucose cotransporter 2 (SGLT2) inhibitor to protect proximal tubular epithelium in vivo and in vitro model of Type 2 diabetes mellitus (T2DM), respectively.

Methods: In vivo, immunohistochemical staining, Masson's trichrome staining and Sirius red staining were used to observe the changes of NNMT expression, renal tubular injury and interstitial fibrosis in renal tissue from the db/db mice. Bioinformatic analysis was also conducted to broaden the range of data validation.

The Novel HLA-DQB1*04:01:01:03 Allele Identified Using HLA-Targeted HiFi Sequencing.

HLA-DQB1*04:01:01:03 differs from HLA-DQB1*04:01:01:01 by a 10-nucleotide fragment insertion in intron 1.

An injectable thermosensitive hydrogel delivering M2 macrophage-derived exosomes alleviates osteoarthritis by promoting synovial lymphangiogenesis.

Osteoarthritis (OA) is a prevalent chronic degenerative disease affecting millions worldwide, with current treatment measures lacking efficacy in slowing disease progression. The synovial lymphatic system (SLS) has emerged as a crucial player in OA pathogenesis, with compromised drainage function contributing to disease advancement. Lymphatic endothelial cells (LECs) within the SLS are influenced by synovial macrophages, whose precise impact on LEC function remains unclear.

Key regulators of vascular calcification in chronic kidney disease: Hyperphosphatemia, BMP2, and RUNX2.

Vascular calcification is quite common in patients with end-stage chronic kidney disease and is a major trigger for cardiovascular complications in these patients. These complications significantly impact the survival rate and long-term prognosis of individuals with chronic kidney disease. Numerous studies have demonstrated that the development of vascular calcification involves various pathophysiological mechanisms, with the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs) being of utmost importance.

Fluorescent Pdots Facilitate High-Resolution Mapping of the Intact Meningeal Vascular Network and Eye-Brain Connections.

Meningeal vascular network is significant in neurology and neurosurgery. However, high-resolution imaging of intact meningeal vascular network is lacking. In this work, we develop a practical experimental method to ensure that the intact meninges are morphologically unfolded and fixed in an agarose gel.

Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology.

and are high-yield forage varieties suitable for cultivation in high-altitude areas of Tibet (4300 m above sea level). was developed from , with the latter serving as its parent variety. was identified as a spring variety, and subsequent RNA sequencing was conducted.

Low-phase-noise microwave generation with a free-running dual-pumped SiN soliton microcomb.

Microwave signals can be generated by photodetecting the repetition frequencies of the soliton microcombs. In comparison to other methods, the dual-pumped method allows for the stable generation of the soliton microcombs even with resonators having lower Q-factors. However, introducing an additional pump laser may affect the phase noise of the generated microwave signals when using these dual-pumped soliton microcombs.

Hepatocyte growth factor attenuates high glucose-disturbed mitochondrial dynamics in podocytes by decreasing ARF6-dependent DRP1 translocation.

Diabetic nephropathy (DN), one of the most common complications of Diabetes Mellitus, is the leading cause of end-stage renal diseases worldwide. Our previous study proved that hepatocyte growth factor (HGF) alleviated renal damages in mice with type 1 Diabetes Mellitus by suppressing overproduction of reactive oxygen species (ROS) in podocytes, while the further mechanism of how HGF lessens ROS production had not been clarified yet. ADP-ribosylation factor 6 (ARF6), the member of the small GTPases superfamilies, is widely spread among epithelial cells and can be activated by the HGF/c-Met signaling.

Effects of Alginic Acid on the Porcine Granulosa Cells and Maturation of Porcine Oocytes.

Scope: Alginic acid (AA) from brown algae is a marine organic compound. There is extensive use of AA in the food industry and healthcare, suggesting a high probability of AA exposure. The present study investigates the effects of AA on porcine ovarian granulosa cells (GCs) and oocytes to explore its mechanism in female reproduction because of its adverse effects on reproduction.

Long non-coding RNA FENDRR suppresses cancer-associated fibroblasts and serves as a prognostic indicator in colorectal cancer.

Genetically abnormal fibroblasts are notably more prevalent in colorectal cancer (CRC) than in adjacent normal tissue, emphasizing their significance in driving the heterogeneity of the tumor microenvironment. Functioning as a significant regulatory gene in the context of fibrosis, FOXF1 adjacent non-coding developmental regulatory RNA (FENDRR) has exhibited abnormal expression in colorectal cancer and interstitial localization in our experiments. However, current research on the role of FENDRR in cancer has focused solely on its impact on cancer cells.

Clinical effects of 3D printing-assisted posterolateral incision in the treatment of ankle fractures involving the posterior malleolus.

Objective: To explore the clinical outcomes of a 3D printing-assisted posterolateral approach for the treatment of ankle fractures involving the posterior malleolus.

Methods: A total of 51 patients with ankle fractures involving the posterior malleolus admitted to our hospital from January 2018 to December 2019 were selected. The patients were divided into 3D printing group (28 cases) and control group (23 cases).

Asymmetric rhodium-catalyzed click cycloaddition to access C-N axially chiral -triazolyl indoles.

The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is regarded as a prime example of "click chemistry", but the asymmetric click cycloaddition of internal alkynes still remains challenging. A new asymmetric Rh-catalyzed click cycloaddition of -alkynylindoles with azides was developed, providing atroposelective access to C-N axially chiral triazolyl indoles, a new type of heterobiaryl, with excellent yields and enantioselectivity. This asymmetric approach is efficient, mild, robust and atom-economic, and features very broad substrate scope with easily available Tol-BINAP ligands.

Immune-check blocking combination multiple cytokines shown curative potential in mice tumor model.

Objective: In order to ensure the stable transcription of target genes, we constructed a eukaryotic high expression vector carrying an immune-check inhibitor PD-1v and a variety of cytokines, and studied their effects on activating immune response to inhibit tumor growth.

Methods: A novel eukaryotic expression plasmid vector named pT7AMPCE containing T7RNA polymerase, T7 promoter, internal ribosome entry site (IRES), and poly A tailing signal was constructed by T4 DNA ligase, on which homologous recombination was used to clone and construct the vector carrying PD-1v, IL-2/15, IL-12, GM-CSF, and GFP. In vitro transfection of CT26 cells was performed, and the protein expression of PD-1v, IL-12 and GM-CSF was detected by Western blot and ELISA after 48 h.

Topological Photonic Lattice for Uniform Beam Splitting, Robust Routing, and Sensitive Far-Field Steering.

Far-field optical beam steering is a fast-growing technology for communications, spatial ranging, and detections. Nonmechanical optical phased arrays based on straight waveguides have been studied recently, where the beam emission angle to the propagation axis can be scanned by conveniently tuning the wavelength. However, the dispersion of the waveguide limits the wavelength sensitivity of beam steering and the deliberately created emitters inevitably introduce in-line backscattering on-chip.

One-Step Synthesis of Self-Stratification Core-Shell Latex for Antimicrobial Coating.

Herein, we describe a one-step method for synthesizing cationic acrylate-based core-shell latex (CACS latex), which is used to prepare architectural coatings with excellent antimicrobial properties. Firstly, a polymerizable water-soluble quaternary ammonium salt (QAS-BN) was synthesized using 2-(Dimethylamine) ethyl methacrylate (DMAEMA) and benzyl bromide by the Hoffman alkylation reaction. Then QAS-BN, butyl acrylate (BA), methyl methacrylate (MMA), and vinyltriethoxysilane (VTES) as reactants and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA) as a water-soluble initiator were used to synthesize the CACS latex.

Transcutaneous auricular vagus nerve stimulation reduces cytokine production in sepsis: An open double-blind, sham-controlled, pilot study.

Background: Studies have shown that vagus nerve-mediated inflammatory reflex could inhibit cytokine production and inflammation in sepsis animals.

Objectives: This study aimed to explore the efficacy of transcutaneous auricular vagus nerve stimulation (taVNS) on inflammation and disease severity of sepsis patients.

Methods: A randomized, double-blind, sham-controlled pilot study was performed.

Promotion of bone formation and antibacterial properties of titanium coated with porous Si/Ag-doped titanium dioxide.

Implant materials are mainly used to repair and replace defects in human hard tissue (bones and teeth). Titanium (Ti) and Ti alloys are widely used as implant materials because of their good mechanical properties and biocompatibilities, but they do not have the ability to induce new bone formation and have no antibacterial properties. Through surface modification, Ti and its alloys have certain osteogenic and antibacterial properties such that Ti implants can meet clinical needs and ensure integration between Ti implants and bone tissue, and this is currently an active research area.

Synthesis of Benzofuran Derivates via a Gold-Catalyzed Claisen Rearrangement Cascade.

A novel method toward a facile synthesis of diverse benzofuran derivates from easily obtained quinols and alkynyl esters has been reported. A gold-catalyzed intermolecular alkoxylation/Claisen rearrangement/condensation cascade was involved. The introduction of difluorodiphenylsilane as a water-trapping reagent in the reaction leads to a higher yield.