Selection of functional sperm by using hyaluronic acid modified magnetic microbeads and an electromagnetic manipulation system.

In recent years, the incidence of infertility has increased year by year. Assisted reproductive technology (ART) is one of the effective strategies to treat infertility. In the process of ART, commonly used methods for sperm separation have shortcomings and there is still room for improvement.

Real-world effectiveness of recombinant zoster vaccine in self-identified Chinese individuals aged ≥50 years in the United States.

We evaluated the vaccine effectiveness (VE) of two doses of recombinant zoster vaccine (RZV) against herpes zoster (HZ) and postherpetic neuralgia (PHN) in Chinese adults at Kaiser Permanente Southern California (KPSC). Chinese KPSC members were identified based on self-reported ethnicity or self-reported preferred spoken/written language. Those aged ≥50 years who received two doses of RZV 4 weeks to ≤ 6 months apart were matched 1:4 to RZV unvaccinated Chinese members and followed through June 2022; second doses were accrued 6/1/2018-12/31/2020.

Prognostic value of JAK3 promoter methylation and mRNA expression in clear cell renal cell carcinoma.

Introduction: Janus kinase 3 (JAK3) is a well-established oncogene in clear cell renal cell carcinoma (ccRCC). The methylation status of oncogene promoters has emerged as biomarkers for cancer diagnosis and prognosis.

Objective: This study aims to investigate the biological and clinical significance of JAK3 promoter methylation in ccRCC.

Influence of revegetation on soil microbial community and its assembly process in the open-pit mining area of the Loess Plateau, China.

Vegetation recovery is an important marker of ecosystem health in the mining area. Clarifying the influence of vegetation recovery on the characteristics of soil microbial community and its assembly process can improve our understanding of the ecological resilience and self-maintaining mechanism in the open-pit mining area. For this purpose, we employed MiSeq high-throughput sequencing coupled with null model analysis to determine the composition, molecular ecological network characteristics, key bacterial and fungal clusters, and the assembly mechanism of the soil microbial communities in shrubs (BL), coniferous forest (), broad-leaved forests (BF), mixed forest (MF), and the control plot (CK, the poplar plantation nearby that had been continuously grown for over 30 a without disturbance).

Polycyclic aromatic hydrocarbon removal from subsurface soil mediated by bacteria and archaea under methanogenic conditions: Performance and mechanisms.

In situ anoxic bioremediation is an easy-to-use technology to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated soil. Degradation of PAHs mediated by soil bacteria and archaea using CO as the electron acceptor is an important process for eliminating PAHs under methanogenic conditions; however, knowledge of the performance and mechanisms involved is poorly unveiled. In this study, the effectiveness and efficiency of NaHCO (CO) as an electron acceptor to stimulate the degradation of PAHs by bacteria and archaea in highly contaminated soil were investigated.

Biochar rebuilds the network complexity of rare and abundant microbial taxa in reclaimed soil of mining areas to cooperatively avert cadmium stress.

Understanding the interactions between the soil microbial communities and species is critical in the remediation of heavy metal-contaminated soil. Biochar has been widely applied as a stabilizer in the remediation of cadmium (Cd)-contaminated soils in mining areas. However, the rebuilding of the microbial taxa of rare and abundant species by biochar and their cooperative resistance to Cd stress remains elusive.

Enhancing network complexity and function of soil bacteria by thiourea-modified biochar under cadmium stress in post-mining area.

Cadmium (Cd) pollution cause severe stress to soil microorganisms and biochar utilized for its ability to immobilize Cd in the soil effectively. However, the influence of biochar on the structure and function of the bacterial network under Cd stress is unclear. This research reports a pot experiment conducted to investigate the impact of 2.

Tai Chi for health and well-being: A bibliometric analysis of published clinical studies between 2010 and 2020.

The objective of this bibliometric review was to identify the volume, breadth, and characteristics of clinical studies evaluating Tai Chi published between January 2010 and January 2020. Five English and four Chinese language databases were searched. Following independent screening, 1018 eligible publications representing 987 studies were identified, which was a three-fold increase from the previous decade.

[Effect of microbial community structure of activated sludge in an Anaerobic-anoxic-oxic process with Actinic reaction enzyme system start-up].

In order to explore the microbial communities and functions of activated sludge in an Anaerobic-anoxic-oxic (A²/O) process under the start-up of Actinic reaction enzyme system (ARES) system and to understand the impact of the ARES system in domestic sewage treatment process, the activated sludge microbial community structure in the A²/O process system before and after ARES system start-up was analyzed by Illumina-HiSeq 2000 high-throughput sequencing platform. By combining with the main parameters related to the effect of sewage treatment, we analyzed the environmental functions of the microbial communities. The microbial community structure of activated sludge was significantly different before and after the ARES system start-up.

Effectiveness of predicting spatial contaminant distributions at industrial sites using partitioned interpolation method.

Soil pollution at industrial sites is an important issue in China and in most other regions of the world. The accurate prediction of the spatial distribution of pollutants at contaminated industrial sites is a requirement for the development of most soil remediation strategies, and is commonly performed using spatial interpolation methods. However, significant and abrupt variations in the spatial distribution of pollutants decrease prediction accuracy.

A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1.

Many animal viral proteins, e.g., Vpr of HIV-1, disrupt host mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1.

Removal of polycyclic aromatic hydrocarbons (PAHs) and the response of indigenous bacteria in highly contaminated aged soil after persulfate oxidation.

Integrated chemical-biological treatment is a promising alternative to remove PAHs from contaminated soil, wherein indigenous bacteria is the key factor for the biodegradation of residual PAHs after the application of chemical oxidation. However, systematical study on the impact of persulfate (PS) oxidation on indigenous bacteria as well as PAHs removal is still scarce. In this study, the influences of different PS dosages (1%, 3%, 6%, and 10% [w/w]), as well as various activation methods (native iron, HO, alkaline, ferrous iron, and heat) on PAHs removal and indigenous bacteria in highly contaminated aged soil were investigated.

Comparison of common spatial interpolation methods for analyzing pollutant spatial distributions at contaminated sites.

Accurate prediction of the spatial distribution of pollutants in soils based on applicable interpolation methods is often the basis for soil remediation in contaminated sites. However, the applicable interpolation method has not been determined for contaminated sites due to the complex spatial distribution characteristics and stronger local spatial variability of pollutants. In this research, the prediction accuracies of three interpolation methods (including the different values of their parameters) for the spatial distribution of benzo[b]fluoranthene (BbF) in four soil layers were compared.

Epidermal growth factor receptor gene mutations in non-small-cell lung cancer cells are associated with increased radiosensitivity in vitro.

Introduction: There is still lack of specific biomarkers in predicting the radiosensitivity of non-small cell lung cancer (NSCLC) patients in clinic. Previous studies have shown that the EGFR gene status may correlate with radiosensitivity of NSCLC. However, the underlying mechanisms remain unknown.

Applications of Recurrent Neural Networks in Environmental Factor Forecasting: A Review.

Analysis and forecasting of sequential data, key problems in various domains of engineering and science, have attracted the attention of many researchers from different communities. When predicting the future probability of events using time series, recurrent neural networks (RNNs) are an effective tool that have the learning ability of feedforward neural networks and expand their expression ability using dynamic equations. Moreover, RNNs are able to model several computational structures.

Effects of electroacupuncture on bladder and bowel function in patients with transverse myelitis: a prospective observational study.

Objective: To preliminarily explore the effect of electroacupuncture (EA) on bladder and bowel dysfunction in patients with transverse myelitis.

Methods: Sixteen participants were treated with EA at bilateral BL32, BL33, and BL35 once a day, five times a week for the first 4 weeks, and once every other day, three times a week for the following 4 weeks. Patients were then followed up for 6 months.

A hybrid intelligent method for three-dimensional short-term prediction of dissolved oxygen content in aquaculture.

A precise predictive model is important for obtaining a clear understanding of the changes in dissolved oxygen content in crab ponds. Highly accurate interval forecasting of dissolved oxygen content is fundamental to reduce risk, and three-dimensional prediction can provide more accurate results and overall guidance. In this study, a hybrid three-dimensional (3D) dissolved oxygen content prediction model based on a radial basis function (RBF) neural network, K-means and subtractive clustering was developed and named the subtractive clustering (SC)-K-means-RBF model.

[Characteristics on the diagnosis and treatment with acupuncture and moxibustion for the improvement of motor sensory function, urination and defecation in myelitis].

The literature was analyzed on the improvement of motor sensory function, urination and defecation in myelitis treated with acupuncture and moxibustion and focused on the characteristics of the diagnosis and treatment. The literature on acupuncture and moxibustion treatment for myelitis was collected from CNKI, Wanfang, VIP and PubMed. The analysis included the characteristics of acupoint selection, methods of acupuncture and moxibustion, time of treatment, total treatment period, efficacy, follow-up, safety, etc.

Evidence base of clinical studies on Tai Chi: a bibliometric analysis.

Background: The safety and health benefits of Tai Chi mind-body exercise has been documented in a large number of clinical studies focused on specific diseases and health conditions. The objective of this systematic review is to more comprehensively summarize the evidence base of clinical studies of Tai Chi for healthcare.

Methods And Findings: We searched for all types of clinical studies on Tai chi in PubMed, the Cochrane Library and four major Chinese electronic databases from their inception to July 2013.

Electrocoagulation pretreatment of wet-spun acrylic fibers manufacturing wastewater to improve its biodegradability.

The electrocoagulation (EC) process was used to pretreat wastewater from the manufacture of wet-spun acrylic fibers, and the effects of varying the operating parameters, including the electrode area/wastewater volume (A/V) ratio, current density, interelectrode distance and pH, on the EC treatment process were investigated. About 44% of the total organic carbon was removed using the optimal conditions in a 100 min procedure. The optimal conditions were a current density of 35.

[Isolation and identification of a pyrene-degrading bacterial strain from contaminated soil].

Objective: The aim of this study was to isolate high-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs) degrading bacterial strains, and to study their degradation potential.

Methods: We used sublimation method to enrich and isolate the degrading bacteria from coking plant samples. Morphological properties, the sequence homology of 16S rRNA and gyrb genes were used to identify the isolated strains.

Genome-wide analysis of cyclin family in rice (Oryza Sativa L.).

The cyclins together with highly conserved cyclin-dependent kinases regulate cell cycle progression in plants. Although extensive and systematic study on cell cycle mechanisms and cyclin functions in yeasts and animals has been carried out, only a small number of plant cyclins have been characterized and classified functionally and phylogenetically. We identified several types of cyclin genes in the rice genome and characterized them by phylogenetic, tandem and segmental duplications analyses.

[Molecular mapping of a bacterial blight resistance gene Xa-25 in rice].

Xa-25 was a bacterial blight resistance gene identified in a somaclonal mutant HX-3. A doubled-haploid (DH) population including 129 stable lines was derived from anther culture of a typical japanica 02428 and a typical indica HX-3 cross. The bacterial blight strain Zhe173, a typical bacterial blight strain in Yangtze River valley, was used to test the resistance or susceptible of the DH population lines, and the results showed that the resistance lines and susceptible lines were 62 and 67, respectively.

[Study on in vitro dissolution rate of geniposide in huangqin qingfei dispersible tablet].

Objective: To study in vitro dissolution rate of geniposide in Huangqin Qingfei dispersible tablet.

Method: A reversed-phase HPLC method was developed for determination of geniposide. In vitro dissolution rates were compared between Huangqin Qingfei dispersible tablet and conventional tablet in the dissolution medium of pH 1.