METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a mA-Dependent Manner.

The development of mammalian skeletal muscle is a highly complex process involving multiple molecular interactions. As a prevalent RNA modification, N6-methyladenosine (mA) regulates the expression of target genes to affect mammalian development. Nevertheless, it remains unclear how mA participates in the development of goat muscle.

HuR Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating Myomaker mRNA Stability.

Human antigen R (HuR) is an RNA-binding protein that contributes to a wide variety of biological processes and diseases. HuR has been demonstrated to regulate muscle growth and development, but its regulatory mechanisms are not well understood, especially in goats. In this study, we found that HuR was highly expressed in the skeletal muscle of goats, and its expression levels changed during longissimus dorsi muscle development in goats.

Hindered rotor benchmarks for the transition states of free radical additions to unsaturated hydrocarbons.

The hindered internal rotors of 32 transition states (TSs) formed through four free radicals, namely methyl, vinyl, ethyl, methoxy (ĊH3, Ċ2H3, Ċ2H5, CH3) additions to acetylene, ethylene, allene, propyne, and propene (C2H2/C2H4/C3H4-a/C3H4-p/C3H6) are studied. To validate the uncertainties of rate constants that stem from the use of different electronic structure methods to treat hindered rotors, the rotations of the newly formed C-C and/or C-O rotors in the transition states are calculated using commonly used DFT methods (B3LYP, M06-2X, ωB97X-D and B2PLYP-D3 with two Pople basis sets (6-31+G(d,p), 6-311++G(d,p)) and cc-pVTZ). The hindrance potential energies V(χ) calculated using the M06-2X/6-311++G(d,p) method are benchmarked at the CCSD(T), CCSD(T)-F12, DLPNO-CCSD(T) levels of theory with cc-pVTZ-F12 and cc-pVXZ (X = T, Q) basis sets and are extrapolated to the complete basis set (CBS) limit.

An ab Initio/Transition State Theory Study of the Reactions of ĊH Species of Relevance to 1,3-Pentadiene, Part II: Pressure Dependent Rate Constants and Implications for Combustion Modeling.

The temperature- and pressure-dependence of rate constants for several radicals and unsaturated hydrocarbons reactions (1,3-CH/1,4-CH/cyCH + Ḣ, CH + ĊH-a, CH + ĊH) are analyzed in this paper. The abstraction reactions of these systems are also calculated and compared with available literature data. ĊH radicals can be produced via Ḣ atom addition reactions to the pentadiene isomers and cyclopentene, and also by H-atom abstraction reactions from 1- and 2-pentene and cyclopentane.

Ab Initio/Transition-State Theory Study of the Reactions of ĊH Species of Relevance to 1,3-Pentadiene, Part I: Potential Energy Surfaces, Thermochemistry, and High-Pressure Limiting Rate Constants.

In this study, the reactions of ĊH radicals are theoretically investigated, with a particular emphasis on hydrogen atom addition reactions to 1,3-pentadiene (CH) to form ĊH radicals, although the subsequent isomerization and decomposition reactions of the ĊH radicals are also of direct relevance to the radicals formed from the pyrolysis and oxidation of species including pentene and cyclopentane. Moreover, H-atom abstraction reactions by hydrogen atoms from 1,3-pentadiene are also investigated. The geometries and frequencies of 63 potential energy surface (PES) minima and 88 transition states are optimized at the ωB97XD/aug-cc-pVTZ level of theory.

Chemical Kinetics of Hydrogen Atom Abstraction from Propargyl Sites by Hydrogen and Hydroxy Radicals.

Hydrogen atom abstraction from propargyl C-H sites of alkynes plays a critical role in determining the reactivity of alkyne molecules and understanding the formation of soot precursors. This work reports a systematic theoretical study on the reaction mechanisms and rate constants for hydrogen abstraction reactions by hydrogen and hydroxy radicals from a series of alkyne molecules with different structural propargyl C-H atoms. Geometry optimizations and frequency calculations for all species are performed at M06-2X/cc-pVTZ level of theory and the hindered internal rotations are also treated at this level.