Chromosomal Location and Identification of as a New Gene Responsible for Familial Bicuspid Aortic Valve.

Congenital bicuspid aortic valve (BAV) signifies the most frequent category of congenital cardiovascular anomaly globally, occurring in approximately 0.5-2% of the general population worldwide. BAV is a major cause of thoracic aortopathy, encompassing aortic stenosis, aortic root dilation with regurgitation, aortic dissection, and aortic aneurysms, consequently leading to substantial late-onset morbidity and mortality.

A Self-Priming Pyroptosis-Inducing Agent for Activating Anticancer Immunity.

Pyroptosis, a form of programmed cell death mediated by the gasdermin family, has emerged as a promising strategy for inducing anti-tumor immunity. However, efficiently inducing pyroptosis in tumor cells remains a significant challenge due to the limited activation of key mediators like caspases in tumor tissues. Herein, a self-priming pyroptosis-inducing agent (MnNZ@OMV) is developed by integrating outer membrane vesicles (OMVs) with manganese dioxide nanozymes (MnNZ) to trigger pyroptosis in tumor cells.

Diffusion Models as Strong Adversaries.

Diffusion models have demonstrated their great ability to generate high-quality images for various tasks. With such a strong performance, diffusion models can potentially pose a severe threat to both humans and deep learning models. However, their abilities as adversaries have not been well explored.

Functional characterization in reveals the role of gene family in bamboo sheath color variation.

Introduction: The basic helix-loop-helix (bHLH) proteins are a large family of transcription factors that are essential to physiology, metabolism, and development. However, the available information is limited about the gene family in , which is widely cultivated in China because of its high-quality and economic value. cultivars exhibit five natural color variations in their shoot sheaths, but the molecular mechanism behind this color diversity remains unclear.

X-Ray Irradiation Induces Oxidative Stress and Upregulates Intestinal Nrf2-Mrp2 Pathway, Leading to Decreased Intestinal Absorption of Valsartan.

It has been documented that radiation can influence the pharmacokinetics of chemotherapy drugs, yet the underlying mechanisms remain poorly understood. In clinical practice, a considerable number of cancer patients undergo radiotherapy, and those with comorbid hypertension required antihypertensive drugs, including valsartan, an angiotensin II receptor blocker. However, there is no research investigating whether radiotherapy poses a risk of altering the pharmacokinetics.

[Design and Reliability Research of Spherical Radiotherapy Accelerator Motion System].

At present, the C-arm structure accelerators commonly used in radiotherapy equipment are complex in operation and have potential safety hazards when realizing non-coplanar treatment. By combining with medical robotic arm technology, a spherical radiotherapy accelerator motion system is designed. The beam module is clamped by the medical robotic arm structure to achieve three-dimensional multi-angle irradiation treatment within the non-coplanar angle range.

Conditional knockdown of hepatic PCSK9 ameliorates high-fat diet-induced liver inflammation in mice.

Instruction: Accumulating evidence has shown that proprotein convertase subtilisin/kexin type 9 (PCSK9) is associated with inflammation in the vascular system. However, the roles of PCSK9 in hepatic inflammation remain unclear. Because PCSK9 is mainly expressed in the liver and modulates lipid uptake through low-density lipoprotein receptor family members, the present study aimed to elucidate the effect of conditional knockdown of hepatic PCSK9 on hyperlipidemia-induced inflammation and the underlying mechanisms of action.

Effect and mechanism of Sanqi () in treating periodontitis.

Objective: To investigate the effect and mechanism of Sanqi () in treating periodontitis.

Methods: The active components and periodontitis targets were analyzed through network pharmacology and molecular docking. A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi () at different doses for 30 d.

Insights into PeERF168 gene in slash pine terpene biosynthesis: Integrating high-throughput phenotyping, GWAS, and transgenic studies.

Resin biosynthesis in conifer is a complex process, controlled by multiple quantitative trait loci (QTLs). Quantifying resin components is traditionally expensive and labor-intensive. In this study, we employed near infrared (NIR) spectroscopy to quantify resin components in Slash pine using 240 genotypes.

First report of causing winged leaf dieback disease on in Yunnan Province, China.

Rhus chinensis, a deciduous tree of the genus Rhus (family Anacardiaceae), is widely cultivated in China for its medicinal, edible, and ornamental value (Zhang et al., 2022). In April 2022, symptoms of winged leaf dieback disease were observed at Southwest Forestry University in Kunming, Yunnan Province, China (E102°45'42.

Chemical imaging unveils mitochondria as the major site of medicinal biguanide accumulation within cells.

Metformin (MET), a commonly prescribed medication for managing type 2 diabetes, has demonstrated various beneficial effects beyond its primary anti-diabetic efficacy. However, the mechanism underlying MET activity and its distribution within organelles remain largely unknown. In this study, we integrate multiple technologies, including chemical labeling, immunostaining, and high-resolution microscopy imaging, to visualize the accumulation of MET in organelles of cultured cells.

Runx2-NLRP3 axis orchestrates matrix stiffness-evoked vascular smooth muscle cell inflammation.

Arterial stiffening is a hallmark of chronic kidney disease (CKD)-related cardiovascular events and is primarily attributed to the elevated matrix stiffness. Stiffened arteries are accompanied by low-grade inflammation, but the causal effects of matrix stiffness on inflammation remain unknown. For analysis of the relationship between arterial stiffness and vascular inflammation, pulse-wave velocity (PWV) and aortic inflammatory markers were analyzed in an adenine-induced mouse model of CKD in chronological order.

Fast and Efficient Screening and Separation Based on AUF-MS Combined With Molecular Docking Technology and Network Pharmacology Method for Potential Xanthine Oxidase Inhibitors From Pinelliae Rhizoma Praeparatum.

Introduction: Studies show that Pinelliae Rhizoma Praeparatum (PRP) has some pharmacological effects in enhancing immunity and against gout.

Objectives: A mathematical model was created for extraction process optimization, analysis and identification, activity screening, and isolation and purification; moreover, the mechanism of action was studied.

Methods: First, the extraction of PRP was investigated using the gray wolf optimization mathematical regression model; the extraction variables were optimized to maximize the yield.

Simulating water and salt changes in the root zone of salt-alkali fragrant pear and the selection of the optimal surface drip irrigation mode.

Faced with the increasingly serious problem of water scarcity, developing precise irrigation strategies for crops in saline alkali land can effectively reduce the negative effects of low water resource utilization. Using a model to simulate the dynamic changes in soil water and salt environment in the root zone of fragrant pear trees in saline alkali land, and verifying them from a production practice perspective with comprehensive benefits as the goal, can optimize the irrigation amount and irrigation technology elements of saline alkali fruit trees, broaden the comprehensive evaluation perspective of decision-makers, and have important significance for improving the yield and production efficiency of forestry and fruit industry in arid and semi-arid areas worldwide. In this study, a two-year field experiment based on three irrigation levels (3000, 3750, and 4500 m·ha) and four emitter discharge rates (1, 2, 3, and 4 L·h) was conducted in Xinjiang, China.

[Distribution Characteristic and Environment Response of DOM in Different Regions of Northern City].

To explore the source information and composition characteristics of dissolved organic matter （DOM） in different regions of water bodies in northern cities, considering the urban water system of Shijiazhuang, Hebei Province as an example, ultraviolet-visible spectroscopy （UV-vis） and three-dimensional fluorescence parallel factor analysis （EEM-PARAFAC） were used to explain the optical parameters, abundance, and proportion of different components of DOM in water bodies of different regions. The results showed that： ① The concentrations of NO-N, NO-N, NH-N, TN, TP, and COD in the upstream were significantly lower than those in urban water bodies and downstream （<0.01）, and TSI increased after the water entered the city.

Prognosis and Management of Recurrent Stenosis After Pulmonary Vein Stenting: A Prospective Study.

Background: Pulmonary vein stenting is effective for severe pulmonary vein stenosis, which is limited by restenosis. The assessment and management of in-stent restenosis (ISR) are inadequate, and follow-up outcomes after reintervention remain unknown.

Objectives: This study aimed to assess the prognosis and management of pulmonary vein ISR and determine whether the modified stent-in-stent strategy is superior to balloon angioplasty (BA) in treating ISR.

Accurate two-step random phase retrieval approach without pre-filtering based on hyper ellipse fitting.

In this work, we propose a hyper ellipse fitting-based high-precision random two-frame phase shifting algorithm to improve the accuracy of phase retrieval. This method includes a process of Gram-Schmidt orthonormalization, followed by a hyper ellipse fitting procedure. The Gram-Schmidt orthonormalization algorithm constructs a quadrature fringe pattern relative to the original fringe pattern.

Enhancing genomic association studies in slash pine through close-range UAV-based morphological phenotyping.

In forestry genetics and industry, tree morphological traits such as height, crown size, and shape are critical for understanding growth dynamics and productivity. Traditional methods for measuring these traits are limited in efficiency, scalability, and accuracy, posing challenges for large-scale forest assessments. This study focuses on integrating unmanned aerial vehicle (UAV) technology with GWAS to improve genomic association studies in slash pine ().

ANK Deficiency-Mediated Cytosolic Citrate Accumulation Promotes Aortic Aneurysm.

Background: Disturbed metabolism and transport of citrate play significant roles in various pathologies. However, vascular citrate regulation and its potential role in aortic aneurysm (AA) development remain poorly understood.

Methods: Untargeted metabolomics by mass spectrometry was applied to identify upregulated metabolites of the tricarboxylic acid cycle in AA tissues of mice.

Insights into the accumulation and hepatobiliary transport of bisphenols (BPs) in liver and bile.

Bisphenols (BPs) are widely distributed in daily life as typical endocrine disruptors. In this study, we examined the distribution of bisphenol A (BPA) and BPA alternatives in liver (n = 149) and bile (n = 102) tissues from the patients with liver cancer, and calculated the hepatobiliary transport efficiency of BPs (T). Seven BPs were detected in both liver (median: 0.

Biocontrol of rusted root rot in Panax ginseng by a combination of extracts from Bacillus amyloliquefaciens YY8 crude protein and Enterobacteriaceae YY115 ethyl acetate.

Background: Rusted root rot is one of the most common root diseases in Panax ginseng, and Cylindrocarpon destructans is one of the main pathogenic fungus. The objective of this study was to screen and explore the extracts of biocontrol bacteria isolated from ginseng rhizosphere soil against Cylindrocarpon destructans.

Results: Bacterial strains Bacillus amyloliquefaciens YY8 and Enterobacteriacea YY115 were isolated and found to exhibit in vitro antifungal activity against C.

Regulation of macroporous cellulose microspheres via phase separation force induced by carbon nanotubes doping for enhanced protein adsorption.

The burgeoning requirement for purified biomacromolecules in biopharmaceutical industry has amplified the exigency for advanced chromatographic separation techniques. Herein, macroporous cellulose microspheres (CCMs) with micron-sized pores are produced by a facile regulation via carbon nanotubes (CNTs). In this strategy, the incorporation of CNTs breaks the homogeneous regeneration of the cellulose, thus providing anisotropic phase force to produce macropores.

MTSC-Net: A Semi-Supervised Counting Network for Estimating the Number of Slash pine New Shoots.

The new shoot density of slash pine serves as a vital indicator for assessing its growth and photosynthetic capacity, while the number of new shoots offers an intuitive reflection of this density. With deep learning methods becoming increasingly popular, automated counting of new shoots has greatly improved in recent years but is still limited by tedious and expensive data collection and labeling. To resolve these issues, this paper proposes a semi-supervised counting network (MTSC-Net) for estimating the number of slash pine new shoots.