One-pot synthesis of a composite consisting of the enzyme ficin and a zinc(II)-2-methylimidazole metal organic framework with enhanced peroxidase activity for colorimetric detection for glucose.

An enzyme-metal organic framework (MOF) composite with saucer-like structure was prepared via one-pot synthesis from ficin (a cysteine proteolytic enzyme with POx activity), zinc(II) ions and 2-methylimidazole. The composites exhibit a 2.5-fold higher catalytic activity and stronger affinity for substrates compared to free ficin.

Competitive method for fluorescent dopamine detection in cerebrospinal fluid based on the peroxidase-like activity of ficin.

Dopamine (DA), a catecholamine neurotransmitter, is considered to be an important indicator for mental diseases detection in the clinic. In this study, a novel fluorescent sensing platform consisting of the ficin-HO-tyramine system for determining DA in cerebrospinal fluids (CSF) was established. The proposed method is based on the fact that ficin, a mimetic peroxidase, can catalyze HO decomposition into OH radicals, which can oxidize non-fluorescent tyramine into fluorescent dityramine.

Enhancing the peroxidase-like activity of ficin via heme binding and colorimetric detection for uric acid.

Ficin, a classical sulfhydryl protease, was found to possess intrinsic peroxidase-like activity. In this paper, we have put forward a novel strategy to improving the peroxidase-like activity of ficin through binding heme. Heme-ficin complexes were successfully obtained by simple one-step syntheticism.

Colorimetric detection of glucose based on ficin with peroxidase-like activity.

In this work, we developed a colorimetric biosensing system for glucose detection by coupling the peroxidase-like of ficin and the glucose oxidase (GOx). GOx can catalyze the oxidation of glucose to produce HO, then, ficin catalyzes the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) by HO to produce a blue color reaction. The present sensing system showed a linear response toward glucose detection over range of 2.