Publications by authors named "Yanina Soledad Bogliotti"
Article Synopsis
- Scientists have successfully derived stable embryonic stem cells (ESCs) from sheep using a specific culture system with FGF2 and IWR1, overcoming previous challenges in livestock.
- Unlike other species, certain culture conditions that work for humans and mice did not effectively maintain sheep pluripotency in vitro.
- The newly derived sheep ESCs exhibit stable characteristics and are crucial for furthering understanding of pluripotency in livestock, which could lead to new biomedical and agricultural advancements.
Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts.
Proc Natl Acad Sci U S A
February 2018
Article Synopsis
- Embryonic stem cells (ESCs) are obtained from the inner cell mass of early-stage embryos and are essential for various agricultural and medical applications.
- Researchers successfully derived stable pluripotent bovine ESCs (bESCs) using a specific culture system that included fibroblast growth factor 2 and a Wnt-signaling pathway inhibitor.
- This method allowed for efficient creation and propagation of bESC lines, which could be used in technologies like nuclear transfer for producing valuable cattle with desirable genetic traits.
Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.
View Article and Find Full Text PDFInterspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans.
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