Ecotoxicological effects of the neonicotinoid insecticide dinotefuran on springtails (Folsomia candida) at soil residual concentration.

Dinotefuran, a third-generation neonicotinoid insecticide, is widely used in agriculture production due to its excellent insecticidal efficacy. Considering its persistence and high toxicity in soil, it is essential to evaluate its low-dose toxic effects on non-target soil organisms such as the springtail (Folsomia candida). The results revealed that the 7-day half lethal concentration (7d-LC) of dinotefuran contact toxicity to springtails was 0.

A Novel Cold-Adapted Catechol 1,2-Dioxygenase From Antarctic Sea-Ice Bacterium Halomonas sp. ANT108: Characterization and Immobilization.

The enzyme catechol 1,2-dioxygenase (CAT) plays a critical role in the biosynthesis pathway of cis, cis-muconic acid (CCMA), which serves as an indispensable raw material for various industrial applications. In this research, we cloned a novel cold-adapted CAT (HaCAT) from the Antarctic sea ice bacterium Halomonas sp. ANT108.

A Novel Cold-Adapted Nitronate Monooxygenase from sp. ANT206: Identification, Characterization and Degradation of 2-Nitropropane at Low Temperature.

Aliphatic nitro compounds cause environmental pollution by being discharged into water with industrial waste. Biodegradation needs to be further explored as a green and pollution-free method of environmental remediation. In this study, we successfully cloned a novel nitronate monooxygenase gene () from the genomic DNA library of sp.

Cold-adapted characteristics and gene knockout of alkyl hydroperoxide reductase subunit C in Antarctic Psychrobacter sp. ANT206.

Alkyl hydroperoxide reductase subunit C (AhpC) contributes to the cellular defense against reactive oxygen species. However, it remains understudied in psychrophiles. Amino acid comparison demonstrated that AhpC from Psychrobacter sp.

Characteristics of cold-adapted carbonic anhydrase and efficient carbon dioxide capture based on cell surface display technology.

Carbonic anhydrase (CA) is currently under investigation because of its potential to capture CO. A novel N-domain of ice nucleoproteins (INPN)-mediated surface display technique was developed to produce CA with low-temperature capture CO based on the mining and characterization of Colwellia sp. CA (CsCA) with cold-adapted enzyme structural features and catalytic properties.

Highly efficient low-temperature biodegradation of polyethylene microplastics by using cold-active laccase cell-surface display system.

To eliminate efficiency restriction of polyethylene microplastics low-temperature biodegradation, a novel InaKN-mediated Escherichia coli surface display platform for cold-active degrading laccase PsLAC production was developed. Display efficiency of 88.0% for engineering bacteria BL21/pET-InaKN-PsLAC was verified via subcellular extraction and protease accessibility, exhibiting an activity load of 29.

Study of fluorescence spectroscopy and molecular mechanisms for the interaction of Hg ions and R-phycoerythrin from marine algae (Porphyra yezoensis).

Heavy metal is a worldwide hazardous material, and many efforts were made to detect them sensitively and selectively. R-phycoerythrin (R-PE), a marine fluorescent protein, is abundant in red algae and participates in photosynthesis. In this work, the fluorescence spectroscopy and molecular mechanism of Hg ions and R-PE were further explored through fluorescence spectrum measurements, time-resolved fluorescence lifetimes, peak fitting of Fourier transform infrared spectroscopy, and molecular docking simulation in this study.

Characteristics and polyethylene biodegradation function of a novel cold-adapted bacterial laccase from Antarctic sea ice psychrophile Psychrobacter sp. NJ228.

Biotreatment of polyethylene (PE) waste is an emerging topic in environmental remediation; in particular, the degrading enzymes requires further exploration. This study described a novel cold-adapted laccase (PsLAC) from an Antarctic psychrophile and characterized its PE-degradation ability. Homology modeling revealed that PsLAC possessed a typical bacterial laccase catalytic structure and unique cold adaptation structural characteristics such as few hydrogen bonds.

Toxicity and risk assessment of nine pesticides on nontarget natural predator Harmonia axyridis (Coleoptera: Coccinellidae).

Background: Harmonia axyridis (Coleoptera: Coccinellidae) is a beneficial predatory arthropod in the agricultural ecosystem. For the success and development of integrated pest management strategies, it is essential to assess the toxicity risks of commonly used pesticides to nontarget arthropods.

Results: The glass tube residue method was used to determine the risk of nine pesticides to H.

Black phosphorous nanomaterials as a new paradigm for postoperative tumor treatment regimens.

Surgery is currently a mainstream treatment modality for various solid tumor indications. However, aggressive resection of tumor tissues frequently causes postoperative complications, which severely undermine the well-being of patients. Moreover, the residue tumor cells may substantially increase the risk of local and distant tumor relapse.

Molecular cloning, characterization, and homology modeling of serine hydroxymethyltransferase from psychrophilic bacterium Psychrobacter sp.

Serine hydroxymethyltransferase (SHMT) plays a significant role in the synthesis of l-serine, purine, and thymidylate, which could be extensively applied in the treatment of cancers and the development of antibiotics. In this study, cloned from Psychrobacter sp. ANT206, a novel cold-adapted SHMT gene (psshmt, 1257 bp) encoding a protein of 418 amino acids was expressed in Escherichia coli.

Evaluation and analysis of the toxicity of mercury (Hg) to allophycocyanin from Spirulina platensis in vitro.

As a global environmental pollution problem, heavy metal pollution has brought great harm to human beings. In this work, we studied the toxicity of Hg on allophycocyanin (APC) at the molecular level. Firstly, APC was extracted and purified from Spirulina platensis mud and its purity (A/A) reached 3.

Three-phase partitioning, a recyclable method for the purification of R-phycoerythrin from a red algae .

In this study, R-phycoerythrin (R-PE) was isolated and characterized from by three-phase partitioning (TPP) method. The effects of temperature, time, pH, salt saturation, and volume ratio on the purity and recovery rate of R-PE were studied. The optimum extraction conditions were determined as follows: salt saturation of 70%, temperature of 25 °C, time of 45 min, pH of 7.

Immobilization of psychrophile Psychrobacter sp. ANT206 onto novel reusable magnetic nanoparticles and its application for nitro-aromatic compounds biodegradation under low temperature.

Efficient biodegradation may offer a solution for the treatment of nitro-aromatic compounds (NACs) with toxicity, mutagenicity and persistence in the environment. In this study, dopamine (DA) functionalized magnetic nanoparticles with biocompatibility and hydrophilicity were synthesized and utilized for the immobilization of nitro-aromatic compounds degrading psychrophile Psychrobacter sp. ANT206 harboring the cold-adapted nitroreductase.

Designing CoS Nanoparticles Anchored on N-Doped Carbon Dodecahedron as Dual-Enzyme Mimics for the Colorimetric Detection of HO and Glutathione.

In recent years, the exploration of the nanozyme, an artificial enzyme with the structure and function of natural enzymes, has become a hot topic in this field. Although significant progress has been made, it is still a huge challenge to design nanozymes with multiple enzyme-like catalytic activities. In this work, we have successfully fabricated a colorimetric sensing platform to mimic peroxidase-like and oxidase-like activities by the CoS nanoparticles decorated N-doped carbon framework porous dodecahedrons (abbreviated to CoS/N-C PDHs).

Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment.

To solve the issue of unsatisfactory recruitment of mesenchymal stem cells (MSCs) around implant in osteoporotic fractures, we fabricated a ROS-responsive system on titanium surface through hydroxyapatite coating and biomolecule grafting. The porous hydroxyapatite and phosphorylated osteogenic growth peptides (-OGP) were introduced onto titanium surface to synergistically improve osteogenic differentiation of MSCs. After the -OGP-promoted expression of osteogenic related proteins, the calcium and phosphate ions were released through the degradation of hydroxyapatite and integrated into bone tissues to boost the mineralization of bone matrix.

Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile sp. ANT206.

Glutaredoxin (Grx) is an important oxidoreductase to maintain the redox homoeostasis of cells. In our previous study, cold-adapted Grx from sp. ANT206 (PsGrx) has been characterized.

Microstructured titanium functionalized by naringin inserted multilayers for promoting osteogenesis and inhibiting osteoclastogenesis.

Osteoporosis is the most common cause of fractures in middle-aged and elderly people. Fracture repair can be difficult due to the decreased bone volume in osteoporosis patients and implants are often required. In this study, a slow-release system for microstructured titanium (Micro-Ti) was designed to promote osteogenesis and inhibit osteoclastogenesis.

Cloning, Expression, Characterization, and Antioxidant Protection of Glutaredoxin3 From Psychrophilic Bacterium sp. ANT206.

Glutaredoxins (Grxs) are proteins that catalyze the glutathione (GSH)-dependent reduction of protein disulfides. In this study, a Grx-related gene (264 bp), encoding a Ps-Grx3, was cloned from sp. ANT206.

The elucidation of the biodegradation of nitrobenzene and p-nitrophenol of nitroreductase from Antarctic psychrophile Psychrobacter sp. ANT206 under low temperature.

Psychrobacter is one important typical strain in the Antarctic environment. In our previous study, Psychrobacter sp. ANT206 from Antarctica with novel cold-adapted nitroreductase (PsNTR) could biodegrade nitrobenzene and p-nitrophenol in low temperature environment.

The rational design of CuSe@(Co,Cu)Se core-shell structures as bifunctional electrocatalysts for neutral-pH overall water splitting.

Highly active and stable bifunctional electrocatalysts for H2 generation from neutral-pH water are desired, but difficult to achieve. The modification of the electronic and crystal structure of a material by element doping, morphology design and constructing a complex is a valid strategy for obtaining high-performance catalysts toward overall water splitting. In this study, a novel Cu2-xSe@(Co,Cu)Se2 core-shell structure with ultrathin (Co,Cu)Se2 nanosheets anchored as a shell on an internal Cu2-xSe core was fabricated, for the first time, by integrating the three above-mentioned modification methods.

Efficient Purification of R-phycoerythrin from Marine Algae () Based on a Deep Eutectic Solvents Aqueous Two-Phase System.

R-phycoerythrin (R-PE), a marine bioactive protein, is abundant in with high protein content. In this study, R-PE was purified using a deep eutectic solvents aqueous two-phase system (DES-ATPS), combined with ammonium sulphate precipitation, and characterized by certain techniques. Firstly, choline chloride-urea (ChCl-U) was selected as the suitable DES to form ATPS for R-PE extraction.

Progress of Iron-Based Nanozymes for Antitumor Therapy.

Artificial nanoscale enzyme-mimics (nanozymes) are promising functional alternatives to natural enzymes and have aroused great interest due to their inherent stability, affordability, and high catalytic ability. Iron-based nanozymes are one of the most investigated synthetic nanomaterials with versatile enzyme-like catalytic properties and have demonstrated remarkable relevance to a variety of biomedical applications, especially biocatalytic therapy against tumor indications. Nevertheless, despite the recent advances in biology and nanotechnology, the therapeutic performance of iron-based nanozymes is still limited by technical issues such as low catalytic efficiency and lack of tumor specificity.

Osteogenesis regulation of mesenchymal stem cells autophagy induced by silica-titanium composite surfaces with different mechanical moduli.

The high surface elastic modulus of the titanium (Ti) implant is one of the critical factors causing poor osteointegration between the implant surface and surrounding bone tissue. To address this challenge, spherical silica nanoparticles (SSNs) and spherical titania nanoparticles (STNs) with different sizes were synthesized and embedded into Ti surfaces via a micro-arc oxidation (MAO) technique. There were no significant changes in the surface roughness and protein adsorption behaviors before and after the embedding of spherical silica nanoparticles and titania nanoparticles into the Ti implant.