Molecular Characterization of the First Partitivirus from a Causal Agent of Dry Rot.

Root rot as a result of is a common root disease caused by spp., which has become one of the main diseases affecting the production of . Currently, several hypovirulence-related mycoviruses have been identified in many phytopathogenic fungi, including spp.

Genome-wide characterization of the bHLH gene family in Gynostemma pentaphyllum reveals its potential role in the regulation of gypenoside biosynthesis.

Background: Gynostemma pentaphyllum, an ancient Chinese herbal medicine, serves as a natural source of gypenosides with significant medicinal properties. Basic helix-loop-helix (bHLH) transcription factors play pivotal roles in numerous biological processes, especially in the regulation of secondary metabolism in plants. However, the characteristics and functions of the bHLH genes in G.

The FomYjeF Protein Influences the Sporulation and Virulence of f. sp. .

causes vascular wilt in more than 100 plant species, resulting in massive economic losses. A deep understanding of the mechanisms of pathogenicity and symptom induction by this fungus is necessary to control crop wilt. The YjeF protein has been proven to function in cellular metabolism damage-repair in Escherichia coli and to play an important role in Edc3 (enhancer of the mRNA decapping 3) function in , but no studies have been reported on related functions in plant pathogenic fungi.

[Identification of terpene synthase gene family in Gynostemma pentaphyllum and expression pattern analysis under abiotic stresses].

The present study aimed to investigate the composition of the terpene synthase(TPS) gene family in Gynostemma pentaphyllum and its role in abiotic stresses. The G. pentaphyllum TPS gene family was identified and analyzed at the genome-wide level using bioinformatics analysis, and the expression patterns of these family members were analyzed in different tissues of G.

Excitation of mirror symmetry higher-order rational soliton in modulation stability regimes on continuous wave background.

We study the relationship between the structures of the nonlinear localized waves and the distribution characteristics of the modulation stability regime in a nonlinear fiber with both third-order and fourth-order effects. On the background frequency and background amplitude plane, the modulation stability region consists of two symmetric curves on the left and right and a point on the symmetry axis. We find that the higher-order excitation characteristics are obviously different at different positions in the modulation stability region.

Phase characters of optical dark solitons with third-order dispersion and delayed nonlinear response.

Dark soliton is usually seen as one of the simplest topological solitons, due to phase shift across its intensity dip. We investigate phase characters of single-valley dark soliton (SVDS) and double-valley dark soliton (DVDS) in a single-mode optical fiber with third-order dispersion and delayed nonlinear response. Notably, two different phase shifts can produce an SVDS with the same velocity under some conditions, which is not admitted for a dark soliton with only the second-order dispersion and self-phase modulation, whose phase shift and velocity is a one-to-one match.

Comparative transcriptomic analysis identifies KcMYB1 as a R2R3-MYB anthocyanin activator in Kadsura coccinea.

Fruit color, as an important appearance attribute, is crucial for attracting consumers. However, the underlying mechanism regulating mature fruit color formation in Kadsura coccinea remains unclear. Here, a comprehensive metabolomics and transcriptomics analysis was performed to investigate the molecular mechanisms of anthocyanin accumulation between two K.

Comparative Pharmacokinetics of Cinobufacini Capsule and Injection by UPLC-MS/MS.

Cinobufacini capsule and injection are two different formulations from the same source, obtained from the extraction of the skin of Cantor, which have been approved by the Chinese State Food and Drug Administration (CFDA) for the treatment of various cancers. Our previous study has found that the cinobufacini capsule and injection exhibited different anticancer effects, but their different pharmacokinetic behaviors, which could give a cause of that, have never been reported. So a sensitive and selective method for the simultaneous quantitation of 13 compounds in the rat plasma, including bufothionine, hellebrigenin, bufalin, gamabufotalin, telocinobufagin, cinobufagin, arenobufagin, cinobufotalin, desacetylcinobufotalin, bufotalin, pseudobufarenogin, resibufogenin, and desacetylcinobufagin, was established by using the Agilent 6460 mass spectrometer equipped with an ESI ion source in a multiple-reaction monitoring (MRM) mode.

Longitudinal Dynamics of Cellular Responses in Recovered COVID-19 Patients.

Safe and effective vaccines and therapeutics based on the understanding of antiviral immunity are urgently needed to end the COVID-19 pandemic. However, the understanding of these immune responses, especially cellular immune responses to SARS-CoV-2 infection, is limited. Here, we conducted a cohort study of COVID-19 patients who were followed and had blood collected to characterize the longitudinal dynamics of their cellular immune responses.

[Analysis of gene variant in a Chinese pedigree with preaxial polydactyly].

Objective: To analyze the pathogenic variant of preaxial polydactyly in a Chinese Han pedigree and identify the cause of polydactyly.

Methods: The peripheral blood DNA of the proband and her parents was extracted. The polydactyly-related genes were detected by trio whole exome sequencing, and the suspected pathogenic gene was screened out.

Source apportionment of carbonaceous aerosols in diverse atmospheric environments of China by dual-carbon isotope method.

Carbonaceous aerosols are major components in PM of both polluted and clean atmosphere. Accurate source apportionment of carbonaceous aerosols may support effective PM control. Dual-carbon isotope method (C and C) was adopted to identify the contribution of three main air pollution sources biogenic and biomass (f), liquid fossil (f) and coal (f).

Multivalley dark solitons in multicomponent Bose-Einstein condensates with repulsive interactions.

We obtain multivalley dark soliton solutions with asymmetric or symmetric profiles in multicomponent repulsive Bose-Einstein condensates by developing the Darboux transformation method. We demonstrate that the width-dependent parameters of solitons significantly affect the velocity ranges and phase jump regions of multivalley dark solitons, in sharp contrast to scalar dark solitons. For double-valley dark solitons, we find that the phase jump is in the range [0,2π], which is quite different from that of the usual single-valley dark soliton.

A Carrier-Free Folate Receptor-Targeted Ursolic Acid/Methotrexate Nanodelivery System for Synergetic Anticancer Therapy.

Purpose: To avoid undefined metabolic mechanisms and to eliminate potential side effects of traditional nanocarriers, new green carriers are urgently needed in cancer treatment. Carrier-free nanoparticles (NPs) based on ursolic acid (UA) have attracted significant attention, but the UA NPs targeting the folate receptor have never been explored. We designed a novel self-assembled UA-Methotrexate (MTX) NPs targeting the folate-receptor and its synergetic anticancer activity was studied in vitro and in vivo.

Abnormal metabolic processes involved in the pathogenesis of non-alcoholic fatty liver disease (Review).

Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases and can lead to liver cirrhosis or liver cancer in severe cases. In recent years, the incidence of NAFLD has increased substantially. The trend has continued to increase and has become a key point of concern for health systems.

Identification of a Novel Phage-Derived Depolymerase and Its Therapeutic Application in Mice.

The rapid expansion of clinical isolates exhibiting resistance to most or all available antibiotics is a global concern. Current treatments for infections caused by this bacterium have become less effective, and the need to explore new alternative therapies is urgent. Depolymerases derived from phages are emerging as attractive anti-virulence agents.

Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid and Specific Identification of Carbapenem-Resistant Strains Harboring , and the Epidemiological Survey of Clinical Isolates.

is an important nosocomial pathogen in hospital-acquired infections, and carbapenem resistance has been increasingly observed worldwide. Oxacillinase production by is a predominant and prevalent carbapenem resistance mechanism of , especially in China. Rapid and specific detection of may offer valuable insight for administration of directed antimicrobial therapy.