Investigating the stability of chimeric murine polyomavirus VP1 Capsomeres via molecular dynamics simulations and experimental analysis.

The development of modular virus-like particle (VLP) vaccine platforms with genetically inserted antigens in viral structural proteins shows great promise for advancing vaccine technology. However, the instability of many constructs leads to trial-and-error approaches, and the challenge of predicting stability based solely on amino acid sequences remains unresolved, yet highly appealing. This study evaluates the stability of wild-type murine polyomavirus (MPV) VP1 capsomeres and three engineered chimeric variants using molecular dynamics (MD) simulations and laboratory experiments.

[Influence of non-depolarizing relaxant on facial nerve monitoring during total intravenous anesthesia].

Objective: To study the feasibility of using non-depolarizing relaxant in intraoperative facial nerve monitoring (IFNM) during total intravenous anesthesia (TIVA).

Methods: Thirty adult patients, aged 37 (20-50), with ASA class I or II, without disorder of facial nerve, underwent IFNM during TIVA, using fentanyl, midazolam, and propofol, to record the compound muscle action potentials (CMAPs) of the facial nerve. Train of four (TOF) pattern was used to evaluate the degree of peripheral neuromuscular blockade.