Hinge-like paper-based dual-channel enzyme-free ratiometric fluorescent microfluidic platform for simultaneous visual detection of carbaryl and glyphosate.

On-site multi-pesticide residues detection is particularly urgent and challenging. Here, we fabricated an enzyme-free ratiometric fluorescent detection system in combination with a hinge-like dual-channel 3D microfluidic paper analytical device (3D μPAD) for simultaneous visual detection of carbaryl and glyphosate. Blue-emission 1-naphthol (Em.

Design of a sustainable light-up flavonol probe for dual-ratiometric fluorescent sensing and visual differentiating ammonia and hydrazine.

Ammonia (NH) and hydrazine (NH) present potential risks to human health, food and environmental safety. A sustainable flavonol-based probe, quercetin pentaacetate (QPA, weak blue emission 417 nm), was fabricated for dual-ratiometric fluorescent sensing and visual differentiating NH and NH. Excited state intramolecular proton transfer-on products with green (487 nm) and yellow (543 nm) emissions occurred as meeting with NH and NH, respectively, for their different nucleophilicities.

A dual-emission carbon dots-based nonenzymatic fluorescent sensing platform for simultaneous detection of parathion-methyl and glyphosate.

Simultaneous and high-performance detection of pesticides is still a considerable challenge and urgent need. Herein, a dual-emission carbon dots (CDs)-based nonenzymatic fluorescent sensing platform has been developed, which shows excellent sensitivity and selectivity in simultaneously detecting parathion-methyl (MP) and glyphosate. CDs with emissions at 440 nm (bCDs) and 660 nm (rCDs) were prepared by hydrothermal treatment of mulberry leaves and sodium hydroxide.

The extraction of phenolic acids and polysaccharides from Thunb. using a deep eutectic solvent.

Establishing a fast and effective extraction method for herbs is beneficial for the determination of their main compounds and estimating their quality. In this study, deep eutectic solvents (DESs) were optimized to simultaneously extract three main types of phenolic acids, i.e.

Rapid identification of Lilium species and polysaccharide contents based on near infrared spectroscopy and weighted partial least square method.

Polysaccharide is the main active compound of Lilium, and showed many activities, such as hypoglycemic, antioxidant, immune-modulatory. There are three types' Lilium in China market, i.e.

Repression of Adipose Tissue Fibrosis through a PRDM16-GTF2IRD1 Complex Improves Systemic Glucose Homeostasis.

Adipose tissue fibrosis is a hallmark of malfunction that is linked to insulin resistance and type 2 diabetes; however, what regulates this process remains unclear. Here we show that the PRDM16 transcriptional complex, a dominant activator of brown/beige adipocyte development, potently represses adipose tissue fibrosis in an uncoupling protein 1 (UCP1)-independent manner. By purifying the PRDM16 complex, we identified GTF2IRD1, a member of the TFII-I family of DNA-binding proteins, as a cold-inducible transcription factor that mediates the repressive action of the PRDM16 complex on fibrosis.

Beige Adipocyte Maintenance Is Regulated by Autophagy-Induced Mitochondrial Clearance.

Beige adipocytes gained much attention as an alternative cellular target in anti-obesity therapy. While recent studies have identified a number of regulatory circuits that promote beige adipocyte differentiation, the molecular basis of beige adipocyte maintenance remains unknown. Here, we demonstrate that beige adipocytes progressively lose their morphological and molecular characteristics after withdrawing external stimuli and directly acquire white-like characteristics bypassing an intermediate precursor stage.