Publications by authors named "Yangwei Ou"
Microcystin-LR (MC-LR), a toxic cyanobacterial toxin in freshwater, poses significant health and ecological risks due to its ability to induce cell apoptosis and liver damage. Sensitive detection of MC-LR is crucial for public health and water safety. In this work, we engineered a multivalent bifunctional nanobody (A2.
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- The study investigates the immune responses in mild and severe cases of mild pneumonia (MPP) using single-cell RNA sequencing of bronchoalveolar lavage fluid (BALF) samples from healthy donors and pediatric patients.
- Severe MPP patients show high levels of exhausted T cells and M1-like macrophages, with T cell exhaustion linked to ongoing type I interferon signaling and lack of support from CD4 T cells.
- In contrast, mild MPP patients have a greater number of activated neutrophils, which enhance protective immunity, highlighting different immune mechanisms in mild versus severe cases.
TIAM2 (T-cell lymphoma invasion and metastasis 2), a RAC1 guanine nucleotide exchange factor, plays crucial roles in human cancer cells. Its homolog, TIAM1, has been reported to promote the migration and invasion of cancer cells through regulating the functions of cancer associated fibroblasts (CAFs). However, the functions of TIAM2 in CAFs have not been investigated.
View Article and Find Full Text PDF[Transfection of hypertrophic cardiac myocytes in vitro with (99)Tc(m)-labeled antisense miR208b oligonucleotide].
Nan Fang Yi Ke Da Xue Xue Bao
August 2015
Objective: To test the efficiency of transfecting (99)Tc(m)-labeled anti-miR208b oligonucleotide into early hypertrophic cardiac myocytes in vitro.
Methods: The anti-oligonucleotide targeting miR208b (AMO) was synthesized and modified with LNA followed by conjugation with N-hydroxysuccinimidyl S-acetyl-meraptoacetyl triglycine (NHS-MAG3) and radiolabeling with (99)Tc(m). NHS-MAG3-LNA-AMO and labeled AMO were purified with Sep-Pak C18 column chromatography, and the former was examined for UV absorption at the 260 nm using Gene Quant DNA/RNA calculator.