Nicking enzyme assisted amplification combined with CRISPR-Cas12a system for one-pot sensitive detection of APE1.

Apurinic/apyrimidinic endonuclease 1 (APE1) is a critical enzyme in the base excision repair (BER) pathway, essential for preserving cellular equilibrium. Variations in APE1 activity within blood or tissues can provide significant insights for clinical cancer screening and disease diagnosis. Consequently, the detection of APE1 activity is critical for clinical diagnostics.

Highly Reusable Enzyme-Driven DNA Logic Circuits.

In recent years, DNA has emerged as a promising molecule for the construction of molecular computing systems. In the research field of DNA logic circuits, enzyme-driven DNA logic circuits, which offer faster reactions and lower complexity, have become the key focus in the field. However, it remains a significant drawback that it lacks the capability of being reused.