iGRLCDA: identifying circRNA-disease association based on graph representation learning.

While the technologies of ribonucleic acid-sequence (RNA-seq) and transcript assembly analysis have continued to improve, a novel topology of RNA transcript was uncovered in the last decade and is called circular RNA (circRNA). Recently, researchers have revealed that they compete with messenger RNA (mRNA) and long noncoding for combining with microRNA in gene regulation. Therefore, circRNA was assumed to be associated with complex disease and discovering the relationship between them would contribute to medical research.

Total Synthesis of Dysifragilones A and B and Dysidavarone C.

The concise synthesis of dysifragilones A and B and dysidavarones has been accomplished for the first time in a divergent way from a common intermediate. The synthetic route features an intramolecular reductive Heck reaction to construct the 6/5/6/6/-tetracycle of dysifragilones A and B and an intramolecular palladium-catalyzed α-arylation of a sterically hindered ketone to forge the tetracyclo[7.7.

GCNCDA: A new method for predicting circRNA-disease associations based on Graph Convolutional Network Algorithm.

Numerous evidences indicate that Circular RNAs (circRNAs) are widely involved in the occurrence and development of diseases. Identifying the association between circRNAs and diseases plays a crucial role in exploring the pathogenesis of complex diseases and improving the diagnosis and treatment of diseases. However, due to the complex mechanisms between circRNAs and diseases, it is expensive and time-consuming to discover the new circRNA-disease associations by biological experiment.

lncRNA- rs2632159 polymorphism could be a biomarker for colorectal cancer susceptibility.

Single-nucleotide polymorphisms (SNPs) in lncRNAs could be biomarkers for susceptibility to colorectal cancer (CRC), but the association of polymorphisms and CRC susceptibility is yet to be studied. Five tagSNPs covering the gene were detected through Kompetitive Allele-Specific PCR among 436 CRC patients and 510 controls. An expression quantitative trait locus (eQTL) bioinformatic analysis was then performed.

LMTRDA: Using logistic model tree to predict MiRNA-disease associations by fusing multi-source information of sequences and similarities.

Emerging evidence has shown microRNAs (miRNAs) play an important role in human disease research. Identifying potential association among them is significant for the development of pathology, diagnose and therapy. However, only a tiny portion of all miRNA-disease pairs in the current datasets are experimentally validated.

[ cloning and comparative analysis of NAD1 gene in three common human parasites].

Objective: To clone the NAD1 gene of three common parasites and analyze their bioinformatics, so as to lay the foundation for further research on the NAD gene.

Methods: By using the cloning method, the full length cDNA (s) of NAD 1 genes of , and Schistosoma japonicum were got, then their physical and chemical properties, compositions of amino acids, subcellular localizations, binary and ternary structures were contrastively analyzed.

Results: The three kinds of NAD1 proteins were similar in the relative molecular weight, subcellular localization, and physical and chemical properties.

The association of polymorphisms in lncRNA- with hepatocellular cancer risk and prognosis.

Hepatocellular cancer (HCC) is one of the major causes of cancer-related mortality. Genetic polymorphisms may affect the susceptibility and clinical outcomes of cancers. We aim to manifest the association of single nucleotide polymorphisms (SNPs) of lncRNA- gene with the risk and prognosis of HCC.

Treatment of Goiter with Traditional Chinese Medicine Regimen Xing Qi Hua Ying Tang: A Clinical Study on 72 Patients with Multinodular and Diffuse Goiter.

Objective: The present work aimed to further explore the therapeutic potential of Xing Qi Hua Ying Tang (XQHYT) by conducting a systemic clinical study in patients with goiter.

Design: Seventy-two patients with either multinodular or diffuse goiter were enrolled and randomly assigned into treatment group receiving XQHYT (n = 36) and control group receiving Hai Zao Yu Hu Tang (n = 36).

Location: Traditional Chinese Medicine Hospital of Shanxi.

Diagnostic accuracy of loop-mediated isothermal amplification in detection of Clostridium difficile in stool samples: a meta-analysis.

Introduction: Clostridium difficile infection (CDI) remains a diagnostic challenge for clinicians. More recently, loop-mediated isothermal amplification (LAMP) has become readily available for the diagnosis of CDI, and many studies have investigated the usefulness of LAMP for rapid and accurate diagnosis of CDI. However, the overall diagnostic accuracy of LAMP for CDI remains unclear.

[Case-control study on T-shaped locking internal fixation and external fixation for the treatment of dorsal Barton's fracture].

Objective: To compare clinical effect of T-shaped locking internal fixation and external fixation in treating dorsal Barton's fracture,and investigate selective strategy of internal fixation.

Methods: From January 2008 to January 2013, 100 patients with dorsal Barton's fracture were randomly divided into two groups. In treatment group, there were 30 males and 20 females with an average age of (33.

[Extraction of fatty constituents from Tabanus bivittatus and their analysis by GC-MS].

Objective: To optimize the extracting condition of fatty constituents from Tabanus bivittatus and analyse by GC-MS.

Methods: Taking fatty yield as index, the extraction technology parameters of fatty constituents of Tabanus bivittatus ere optimized by single factor test and orthogonal test.

Results: The optimum extraction parameters were as follows: the ratio of acetone to petroleum ether 1: 2, extraction time 2 h, solid-liquid ratio 1: 50.

[Study on acting mechanism of anti-morphine conditioned place preference between aqueous extract of Corydalis yanhusuo and L-THP and comparison of their effects].

Objective: To study the acting mechanism of anti-morphine conditioned place preference (CPP) between aqueous extract of Corydalis yanhusuo and L-THP and compare their effects.

Method: The CPP model was established by injecting morphine in rats with a increasing dose for 10 days, with the initial dose of 10 g x kg(-1) and the final dose of 100 g x kg(-1), 10 mg x kg(-1) was increased each day, thus 100 mg x kg(-1) was injected by d 10. Having been treated with differential doses (2, 1 and 0.

Further studies on the chemical constituents of Chinese folk medicine Gentiana apiata N.E. Br.

One new secoiridoid glycoside with conjugated diene, named 3-epi-swertiajaposide C (1), was isolated from the whole plants of Gentiana apiata N.E. Br.

Comparative proteomics and correlated signaling network of rat hippocampus in the pilocarpine model of temporal lobe epilepsy.

In temporal lobe epilepsy (TLE), the seizure origin typically involves the hippocampal formation. The pilocarpine-induced TLE provides a model to investigate the molecular and functional characterization of epileptogenesis by mimicking the human epileptic condition. Here, we employed a 2-D gel-based proteomic technique to profile proteome changes in the rat hippocampus after pilocarpine treatment.

The estimation of the number of underground coal miners and the annual dose to coal miners in China.

This paper introduces an estimation method for the number of underground coal miners and the annual dose to coal miners in China. It shows that there are about 6 million underground miners at present and the proportion is about 1, 1 and 4 million for national key coal mines, state-owned local coal mines, and township and private-ownership coal mines, respectively. The collective dose is about 1.

[Bioinformatics studies on photosynthetic system genes in cyanobacteria and chloroplasts].

This study compared homology of base sequences in genes encoding photosynthetic system proteins of cyanobacteria (Synechocystics sp. PCC6803, Nostoc sp. PCC7120) with these of chloroplasts (from Marchantia Polymorpha, Nicotiana tobacum, Oryza sativ, Euglena gracilis, Pinus thunbergii, Zea mays, Odentella sinesis, Cyanophora paradoxa, Porphyra purpurea and Arabidopsis thaliana) by BLAST method.