Clinical characteristics and risk factors of intracranial hemorrhage after spinal surgery.

Background: Intracranial hemorrhage after spinal surgery is a rare and devastating complication.

Aim: To investigate the economic burden, clinical characteristics, risk factors, and mechanisms of intracranial hemorrhage after spinal surgery.

Methods: A retrospective cohort study was conducted from January 1, 2015, to December 31, 2022.

BC047440 overexpression is a risk factor for tumor invasion and poor prognosis in hepatocellular carcinoma.

Background/aims: The aim of this study was to investigate the relationship between a new tumor-relative protein (BC047440) expression and clinic pathological parameters of hepatocellular carcinoma (HCC), and to evaluate the prognostic value of BC047440 for HCC patients.

Methods: Following the prokaryotic expression and polyclonal antibodies generation of BC047440, two methods, including western blot and immunohistochemical staining were employed to detect BC047440 expression in 68 HCC specimens. The correlation between BC047440 expression and clinicopathologic outcome, and prognostic value of BC047440 for HCC patients were analyzed.

[Screening and identification for cDNA of differentially expressed genes in human primary hepatocellular carcinoma].

Objective: Screening and identification of differentially expressed genes in human primary hepatocellular carcinoma(HCC).

Methods: The differentially expressed genes subtracted cDNA library of HCC constructed by suppression subtractive hybridization(SSH) technique was screened by colony in situ hybridization, then the positive clones were further screened with PCR amplification. The positive clones were sequenced and analyzed for homology in the Genbank databases with Basic Local Alignment Search Tool BLAST .