New 20 m Progressive Shuttle Test Protocol and Equation for Predicting the Maximal Oxygen Uptake of Korean Adolescents Aged 13-18 Years.

: Although several equations for predicting VO in children and adolescents have been reported, the validity of application of these equations to the Korean population has not been verified. The purpose of study was to develop and validate regression models to estimate maximal oxygen uptake (VO) using a newly developed 20 m progressive shuttle test (20 m PST) protocol in Korean male ( = 80, 15.3 ± 1.

In vitro chondrocyte culture in a heparin-based hydrogel for cartilage regeneration.

In the present study, we applied and optimized a heparin-based hydrogel system, formed by thiolated heparin and diacrylated poly (ethylene glycol), for three-dimensional chondrocyte culture. Encapsulation by the heparin-based hydrogel did not affect the chondrocyte viability (better than calcium-induced alginate gel), and the heparin-based hydrogel promoted chondrocyte proliferation, while maintaining chondrogenic nature. Phenotypic analyses, such as glycosaminoglycan accumulation and histological staining, also supported the proper role of the heparin-based hydrogel for cartilage regeneration; a continuous increase in glycosaminoglycan amount was observed during the culture period.

Formation of a novel heparin-based hydrogel in the presence of heparin-binding biomolecules.

An injectable, heparin-based hydrogel system with the potential to be gelled with cells was developed. First, heparin was modified to have thiol groups by the modification of carboxylic groups of heparin with cysteamine using carbodiimide chemistry. Thiol functionalization of heparin carboxylic groups was controlled from 10% to 60% of the available COOH groups, and the retained bioactivity of the modified heparin, characterized by its binding affinity to antithrombin III, decreased with increasing functionalization.

Allele frequencies and haplotypes of the STR loci of the PowerPlex Y-system in southern populations from Korea.

In this study, 12 Y-specific STR loci--DYS391, DYS389 I, DYS439, DYS389 II, DYS438, DYS437, DYS19, DYS392, DYS393, DYS390, DYS385a/b--were analyzed in 259 unrelated males from the southern populations in Korea using the PowerPlex Y PCR system.