A novel halolysin without C-terminal extension from an extremely halophilic archaeon.

Halolysins are extracellular proteases secreted by halophilic archaea for nutritional purposes. They bear great application potentials in various industries. Yet the diversity of halolysins remains underexplored.

Natronorubrum halophilum sp. nov. isolated from two inland salt lakes.

Two halophilic archaeal strains, SHR37 and NEN6, were isolated from salt lakes located in the Tibet and Xinjiang regions of China. The two strains were found to form a single cluster (99.9% and 99.

sp. nov., isolated from marine solar salterns.

Three halophilic archaeal strains, YJ-53, ZS-5 and DYF38, were isolated from marine solar salterns located in different provinces of China. The three strains formed a single cluster (99.7-99.

Salinirubellus salinus gen. nov., sp. nov., isolated from a marine solar saltern.

A halophilic archaeal strain, designated ZS-35-S2, was isolated from Zhoushan marine solar saltern in Zhejiang Province, China. Cells were pleomorphic, Gram-stain-negative and formed red-pigmented colonies on agar plates. The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v).