Biofilm-disrupting DNA nanomedicines for targeted elimination of resistant wound microbiota.

Biofilms are complex bacterial communities that significantly hinder the treatment of chronic and recurrent infections by enhancing bacterial virulence and conferring resistance to antimicrobial therapies. To address this challenge, an intelligent DNA nanomedicine has been engineered to dismantle biofilms and target resistant bacteria, offering an innovative solution for chronic wound infections. These nanomedicines initiate biofilm degradation through in situ generation of potent oxidative radicals, enabling deep biofilm penetration and precise bacterial targeting.

Spermidine alleviates copper-induced oxidative stress, inflammation and cuproptosis in the liver.

Copper exposure poses potential detrimental effects on both public and ecosystem health. Spermidine, an antioxidant, has shown promise in reducing oxidative stress and inflammation within the liver. However, its specific role in mitigating copper-induced hepatic cuproptosis and disturbances in copper metabolism remains unexplored.

Cerium-doped Prussian blue biomimetic nanozyme as an amplified pyroptosis inhibitor mitigate Aβ oligomer-induced neurotoxicity in Alzheimer's disease.

Antioxidant enzyme therapy shows promise for treating Alzheimer's disease (AD), but significant challenges remain in achieving effective blood-brain barrier (BBB) penetration and sustained therapeutic effects. We developed a novel neutrophil membrane (NM)-coated cerium-doped Prussian blue biomimetic nanozyme (NM@PB-Ce) that demonstrates outstanding enzymatic properties and targeted therapeutic efficacy. Extensive physicochemical characterization using transmission electron microscopy, X-ray photoelectron spectroscopy, and dynamic light scattering confirmed the successful synthesis of uniform nanoparticles (~ 142 nm) with preserved membrane protein functionality.

[Experimental study on injection completion rate and performance for needle-free insulin injection].

As a relatively novel technique for drug delivery, the needle-free injection technique is characterized by transporting the drug liquid to the designated subcutaneous position through a high-speed micro-jet. Although this technique has been applied in many fields, the research on its drug dispersion mechanism and injection performance is insufficient. The presented study aims to identify critical parameters during the injection process and describe their influence on the injection effect.

First-principles study on energetic cocrystals of CL-20/4,5-MDNI with two different stoichiometric ratios under high pressure.

Context: This research determined the crystal structure, molecular structure, electronic structure, optical properties, mechanical properties, and Hirshfeld analysis of the CL-20/4,5-MDNI cocrystal at two distinct stoichiometric ratios under hydrostatic pressures varying from 0 to 100 GPa. The findings revealed that the CL-20/4,5-MDNI cocrystal with a 1:1 ratio experienced two structural transitions at pressures of 80 GPa and 90 GPa. Notably, new covalent bonds, C10-O13 and C9-O14, were established, whereas the C10-H10C bond was disrupted.

Genetic evidence identifies a causal relationship between EBV infection and multiple myeloma risk.

Background: Previous observational studies have suggested a potential association between Epstein-Barr virus (EBV) infection and the development of multiple myeloma (MM), but this relationship is not clear. Therefore, we conducted a systematic Mendelian randomization (MR) analysis to investigate the causal relationship between EBV infection and the risk of MM, while exploring the possible mediating role of immune cells in this association.

Methods: The study first conducted a two-sample MR analysis using the MM R11 dataset from the FinnGen Consortium to evaluate the causal relationship between five EBV infection-related antibodies (AEB-IgG, EA-D, EBNA-1, VCA-p18, and ZEBRA) and MM, with validation in the MM R10 dataset.

Characteristics analysis of microstructure and physicochemical properties of fresh and water-soaked fish maws derived from various swim bladder species.

Fish maw, a traditional nutritious food, has significant development potential. However, the limited species sources and scarce research on processing characteristics present challenges. This study selected nine species of fish maws to determine their morphology and yield during hydrothermal treatment.

Blue light restores functional circadian clocks in eyeless cave spiders.

Evolution in profound darkness often leads to predictable, convergent traits, such as the loss of vision. Yet, the consequences of such repeated evolutionary experiments remain obscure, especially regarding fundamental regulatory behaviors like circadian rhythms. We studied circadian clocks of blind cave spiders and their sighted relatives.

Strontium-based quaternary ammonium salt chitosan particles for ultrafast hemostasis of open fracture.

The management of open bone defects poses a formidable challenge in clinical practice, primarily due to issues such as profuse bleeding, inflammation, bacterial infections, and compromised bone fracture healing. To tackle these complexities, we have devised a novel hemostatic powder, namely the compound strontium-based chitosan quaternary ammonium salt hemostatic powder (QCS@SrT-TA). This innovative powder leverages the self-assembly of tripolyphosphate and strontium ions, facilitated by a positively charged core cross-linker.

Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications.

As an alternative compound of bisphenol A (BPA), bisphenol B (BPB) was widely used in plastic materials. The potential actions of BPB on the function of Leydig cells through the regulation of H3K27me3 and H3K9me3 remains unclear. Our goal was to assess how BPB influences Leydig cell function via histone modifications mediated by H3K27me3 and H3K9me3.

Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat ( L.).

Phytochromes are essential photoreceptors in plants that sense red and far-red light, playing a vital role in regulating plant growth and development through light signal transduction. Despite extensive research on phytochromes in model plants like and rice, they have received relatively little attention in wheat. In this study, we employed bioinformatics methods to identify eight genes in the Aikang58 wheat variety.

Inhibition of FTO expression by thiodiphenol impairs Leydig cell maturation in pubertal male rats.

This study investigated the multifaceted effects of 4,4'-thiodiphenol (TDP) on male reproductive health, focusing on its toxicity, hormonal modulation, and impact on steroidogenesis. Male rats were exposed to TDP at varying doses (0, 1, 10, and 100 mg/kg) from day 35-56 postpartum. Overt toxicity was evident as TDP significantly reduced inhibited spermatogenesis at 10 and 100 mg/kg.

Modulating room-temperature phosphorescence of D-π-A luminogens via methyl substitution, positional isomerism, and host-guest doping.

Organic room-temperature phosphorescence (RTP) luminogens have showed significant potential in the fields of diagnostics, sensing, and information encryption. However, it is difficult to achieve high RTP yield (Φ) and long RTP lifetime simultaneously. By methyl substitution, positional isomerism, and host-guest doping, three new D-π-A type luminogens named as TBTDA, 2M-TBTDA, and 3M-TBTDA were designed and synthesized, whose RTP properties were tuned and optimized.

Inducers of Autophagy and Cell Death: Focus on Copper Metabolism.

Copper is an essential trace element in biological systems, playing a key role in various physiological functions, including redox reactions and energy metabolism. However, an imbalance in copper homeostasis can induce oxidative stress, mitochondrial dysfunction, and inhibition of the ubiquitin-proteasome system, ultimately leading to significant cytotoxicity and cell death. According to recent research, copper can bind to lipoylation sites on proteins involved in the tricarboxylic acid cycle, causing aggregation of lipoylated proteins, the loss of Fe-S cluster proteins, proteotoxic stress, and ultimately, cell death.

Symptom-related experience and sexual health of female patients with pulmonary arterial hypertension: protocol for a systematic review and qualitative meta-synthesis.

Introduction: Pulmonary arterial hypertension (PAH) is a complex condition affecting quality of life, characterised by high blood pressure in the pulmonary arteries leading to heart strain. PAH's impact extends beyond physical symptoms, influencing emotional and social well-being, particularly in women where it affects sexual health and pregnancy outcomes. Despite medical advancements, the disease's full impact on women's lives is under-researched, especially regarding sexual experiences and health.

Impact of triclocarban exposure on development and gene expression in the wolf spider, Pardosa pseudoannulata (Araneae: Lycosidae).

Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to water resources and ecosystems. The wolf spider Pardosa pseudoannulata (Araneae: Lycosidae) is a dominant predator typically inhabiting rice fields or wet habitats near water sources. However, little is known about the effects of TCC on the wolf spiders.

Comparing the antioxidant effects of single and binary combinations of Lactiplantibacillus plantarum in vitro and in vivo and their application in yogurt.

Lactiplantibacillus plantarum have been studied for their antioxidant properties, which can mitigate oxidative stress and improve health outcomes. The study aimed to compare the antioxidant properties of single and binary L. plantarum and their impact on yogurt.

Effects of transcranial magnetic stimulation combined with extracorporeal shockwave therapy for poststroke spasticity: study protocol for a randomised controlled trial.

Introduction: Spasticity is a common complication of stroke, which is related to poor motor recovery and limitations in the performance of activities. Both transcranial magnetic stimulation (TMS) and extracorporeal shockwave therapy (ESWT) are effective treatment methods for poststroke spasticity (PSS). However, there is no existing study exploring the safety and effectiveness of TMS combined with ESWT for PSS.

Optimized MALDI2-Mass Spectrometry Imaging for Stable Isotope Tracing of Tissue-Specific Metabolic Pathways in Mice.

Spatial stable isotope tracing metabolic imaging is a cutting-edge technique designed to investigate tissue-specific metabolic functions and heterogeneity. Traditional matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) techniques often struggle with low coverage of low-molecular-weight (LMW) metabolites, which are often crucial for spatial metabolic studies. To address this, we developed a high-coverage spatial isotope tracing metabolic method that incorporates optimized matrix selection, sample preparation protocols, and enhanced post-ionization (MALDI2) techniques.

A survey of attitudes towards the curriculum for clinical medicine postgraduates pursuing professional master's degrees: perspectives of supervisors and students.

Background: Based on the recommendation of the Ministry of Education in China to differentiate between "academic" and "professional" degrees, medical schools offer both professional and academic degrees for postgraduates. In China, clinical postgraduates who are pursuing a professional master's degree also participate in standardised residency training (SRT). However, little attention has been given to feedback from students and supervisors regarding postgraduate curricula.

Efficacy and safety of different progestogens in women with first threatened miscarriage: A network meta-analysis.

Threatened miscarriage is defined as early vaginal bleeding before 12 weeks of gestational age and can occur in any pregnancy regardless of maternal age, race, comorbidities, lifestyle, or socioeconomic status, and about one-quarter of threatened miscarriages proceed to complete miscarriage. To assess the relative effectiveness and safety of different progestogens in women with first threatened miscarriage, using a network meta-analysis. A systematic search was conducted in PubMed, EMBASE, and Cochrane Library databases from inception to April 2023.

Rotavirus-Inspired Nanointerface Engineered Biosensors for All-in-One Cancer Diagnosis.

Ultrasensitive and population-scale cancer screening technologies are critical to reducing cancer mortality. However, the current qRT-PCR falls short in high-throughput screening of multiple cancers. Here, a rotavirus-inspired multicancer diagnosis system (RMDS) is developed via nanointerface engineering.

Structure-activity relationship and in silico docking analysis of dicarboximide fungicides on 17β-hydroxysteroid dehydrogenase 1 of human, rat, and pig.

Dicarboximide fungicides, including captafol, captan, cyclohexylthiophthalimide, folpet, and procymidone, represent a distinct category of fungicides. 17β-Hydroxysteroid dehydrogenase 1 (17β-HSD1) catalyzes the conversion of estrone to estradiol in mammals. Yet, the impact of these fungicides on 17β-HSD1 activity remains unknown.

Cyclopiazonic acid suppresses the function of Leydig cells in prepubertal male rats by disrupting mitofusin 1-mediated mitochondrial function.

This research investigated the impact of cyclopiazonic acid (CPA), a mycotoxin, on the function of progenitor Leydig cells (PLCs) in prepubertal male rats, focusing on its potential disruption of mitochondrial integrity through mitofusin 1 (MFN1) modulation. In vivo, Sprague Dawley rats received CPA (0.2, 1, 5 mg/kg/day) via gavage from postnatal days 21-28 to evaluate PLC function and mitochondrial morphology using serum hormone levels, histology, qPCR, and Western blot analyses.