Compliance with rigid gas-permeable contact lens use in children younger than 6 years in China: a 6-year retrospective study.

Purpose: To explore the abandonment rate and factors influencing the use of rigid gas-permeable contact lenses (RGPCL) among children aged < 6 years.

Methods: This retrospective case series study included 70 children aged < 6 years who were fitted with RGPCL for visual rehabilitation between January 2016 and December 2021. We collected data on indications, discontinuation rates, and reasons for discontinuation from medical records and via telephone calls and investigated the factors influencing contact lens abandonment.

Longitudinal assessment of the progression of severe keratoconus based on corneal topography.

We aimed to assess long-term disease progression in patients with severe keratoconus (KC). Clinical records of 125 patients (201 eyes) with severe KC followed-up for > 12 months were retrospectively analyzed. From these, 28 patients (31 eyes) were included.

Rigid Gas-Permeable Contact Lens for Visual Rehabilitation in Children Younger Than 12 Years With Penetrative Ocular Trauma.

Objectives: To observe the clinical outcomes of visual rehabilitation using rigid gas-permeable contact lenses (RGPCLs) after penetrative ocular trauma in children younger than 12 years in China.

Methods: Patients younger than 12 years with penetrative ocular trauma fitted with an RGPCL for visual rehabilitation from 2017 to 2021 were included. In the case cohort, the best-corrected visual acuity (BCVA) with spectacles was measured when the RGPCL was fitted, and the initial BCVA with RGPCL, and the BCVA at the last visit were compared.

Natural Nano-Drug Delivery System in Extract with Modified Berberine Hydrochloride Pharmacokinetics.

Purpose: This study aimed to evaluate the pharmaceutical and pharmacokinetic effects of the natural nanoparticles (Nnps) isolated from extract on berberine hydrochloride (BBR) and systematically explore the related mechanisms.

Methods: Firstly, Nnps were isolated from extract and then an Nnps-BBR complex was prepared. After qualitative and quantitative analysis in terms of size, Zeta potential, morphology, and composition of the Nnps and the Nnps-BBR complex, the effects of the Nnps on the crystallization of BBR were characterized.

Palladium-Catalyzed Desymmetric Intermolecular C-N Coupling Enabled by a Chiral Monophosphine Ligand Derived from Anthracene Photodimer.

The development of chiral ligands with privileged scaffolds plays an important role in transition-metal-catalyzed asymmetric reactions. Herein we present anthracene-photodimer-derived chiral monophosphine ligand , which features dual chirality and a rigid scaffold. This ligand exhibits remarkable efficiency in Pd-catalyzed desymmetric intermolecular C-N coupling under mild conditions with excellent chemo- and enantioselectivity.

Sequential Cross-Coupling/Annulation of ortho-Vinyl Bromobenzenes with Aromatic Bromides for the Synthesis of Polycyclic Aromatic Compounds.

A sequential cross-coupling/annulation of ortho-vinyl bromobenzenes with aromatic bromides was realized, providing a direct and modular approach to access polycyclic aromatic compounds. A vinyl-coordinated palladacycle was proposed as the key intermediate for this sequential process. Excellent chemoselectivity and regioselectivity were observed in this transformation.

Synthesis and Characterization of a Pentiptycene-Derived Dual Oligoparaphenylene Nanohoop.

Structural designs combining oligoparaphenylene-derived nanohoops with other functional organic building blocks should lead to novel molecular architectures with intriguing properties. Herein, we describe the synthesis of a pentiptycene-derived chiral dual nanohoop molecule with key steps including ring expansion through dianthracene cycloreversion and transannular [4+2] cycloaddition across a 64-membered macrocycle. The crystal structure of the nanohoop molecule displays an ordered packing pattern with long-range channels in the solid state.

Asymmetric Alkenylation of Enones and Imines Enabled by A Highly Efficient Aryl to Vinyl 1,4-Rhodium Migration.

The asymmetric rhodium-catalyzed alkenylation of enones and imines with arylboronic acids has been developed. A highly controllable aryl to vinyl 1,4-rhodium migration is the key step. Stereodefined vinyl moieties were installed in excellent enantioselectivies for most examined examples.

[Effect of cystatin from on DSS - induced ulcerative colitis in mice].

Objective: To investigate the effect of cysteine protease inhibitor derived from (SjCystatin) on dextran sodium sulfate (DSS)-induced acute ulcerative colitis in mice.

Methods: Eighteen C57BL/6 mice were randomly divided into three groups: a control group treated with PBS (Group A), a DSS-induced-colitis group treated with PBS (Group B), and a DSS-induced-colitis group treated with SjCystatin (Group C). Colitis was induced in mice by giving 3% DSS orally for 7 days.

[Intervention with Schistosoma japonicum cysteine protease inhibitor for treatment of lipopolysaccharide-induced sepsis in mice].

Objective: To observe the effect of Schistosoma japonicum cysteine protease inhibitor (rSjCystatin) for treatment of lipopolysaccharide (LPS)-induced sepsis in mice.

Methods: After a week of adaptive feeding, 54 BALB/c mice were randomly divided into normal control group (group A), sepsis group (group B), and rSjCystatin intervention group (group C). The mice in group A received an intraperitoneal injection of PBS (100 µL), and those in groups B and C were injected with PBS (100 µL) containing LPS (10 mg/kg); the mice in group C were also intraperitoneally injected with 25 µg sjCystatin in 100 µL PBS 30 min after LPS injection.

[Experience and analysis of excellent works of human parasitology in national medical colleges and universities].

Teaching competition is an effective way for college and university teachers to improve their teaching skills. Based on the teaching practice and experience in medical parasitology, this paper discusses several key issues in teaching competition including topics, teaching designs and teaching methods. It provides references for the teachers in department of parasitology of universities and colleges to improve the quality of classroom teaching.

[Anti-tumor effect of infection on melanoma in mice].

Objective: To explore the anti-tumor effect of 17XL strains of (P.y) infection on melanoma in mice.

Methods: B16F10 tumor cells were axillarilly injected into the right flank of 20 C57BL/6 mice to establish tumor-bearing mouse models.

[Component analysis of excretory/secretory protein from Trichinella spiralis adult worm].

Objective: To analyze the component of adult worm excretory/secretory protein(AWESP) from Trichinella spiralis using the shotgun method, and find out the active component underlying its regulatory effect on colitis in humans.

Methods: The T. spiralis AWESP was prepared, separated by SDS-PAGE, lysed with trypsin, and analyzed by shotgun LC-MS/MS.

[Effect of exogenous nitric oxide on antioxidants from mice infected with ].

Objective: To study the effect of exogenous nitric oxide donor sodium nitroprusside (SNP) on antioxidant enzymes activities and lipid peroxidation of mice infected with .

Methods: BALB/c mice were infected with separated by the digestion method. Forty-two days post-infection, the peripheral blood and hepatic tissue from the infected or normal mice were collected.

Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide.

Objective: To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro.

Methods: All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of Bax, Bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified.

[Effect of Trichinella spiralis and its worm-derived proteins on CLP-induced sepsis in mice].

Objective: To observe the effect of Trichinella spiralis and its worm-derived proteins on cecal ligation and puncture (CLP)-induced sepsis in mice.

Methods: Eighty male BALB/c mice were randomly divided into sham-operated group, CLP group, Trichinella spiralis muscle larvae (ML) pre-infection group (ML+CLP group), soluble muscle larvae proteins (SMP) treatment group (SMP+CLP group) and excretory-secretory proteins (MES) treatment group (MES+CLP group). In ML+CLP group, the mice were orally infected with 300 Trichinella spiralis muscle larvae at 28 days before CLP and those in the other groups were intraperitioneally injected with PBS or SMP (25 µg/mice) or MES (25µg/mice) 30 min after CLP.

Synthesis of Oligoparaphenylene-Derived Nanohoops Employing an Anthracene Photodimerization-Cycloreversion Strategy.

The century-old yet synthetically underexplored anthracene photodimerization-cycloreversion reactions have been employed as the key steps to access highly strained aromatic hydrocarbons. Herein we report the chemical syntheses of oligoparaphenylene-derived nanohoops in five steps or less featuring a rigid dianthracene synthon. The newly synthesized nanohoops display intriguing experimental and computational properties.

[Immunomodulatory Effect of Trichinella spiralis and its Derivative Products in Allergy and Autoimmune Diseases].

Trichinella spiralis and its derivative antigens stimulate T cell activation by inducing dendritic cell semimaturation, thereby regulating the Th1/Th2 type immune response and alleviating or inhibiting allergy and autoimmune diseases. The regulatory T cell and anti-inflammatory factors play important roles in this immunomodulatory process. This article reviews the modulatory effects and their mechanisms of Trichinella spiralis and its derivatives in autoimmune disorders.

[In vitro Effect of Hypericin against Toxoplasma gondii Tachyzoites].

Objective: To investigate the killing effect of hypericin on tachyzoites of Toxoplasma gondii RH strain in vitro.

Methods: Normal saline （group A） and different concentrations of hypericin (5 μg/ml, group B; 50 μg/ml, group C; 500 μg/ml, group D） were added to T. gondii tachyzoites in 24-well plate（1×10(6)/well）.

[Effect of excretory/secretory protein of adult worm on CLP-induced sepsis in mice].

Objective: To observe the effect of excretory/secretory products from adult worms (AES) on cecal ligation and puncture (CLP) -induced sepsis in mice.

Methods: Forty-eight BALB/c mice were randomly divided into 3 groups：a sham operation group (PBS + sham group, Group A), a CLP-induced sepsis group (PBS+CLP group, Group B) and an AES treatment group (AES+CLP group, Group C). The mice of each group were intraperitoneally injected with 25 μg of AES or PBS only as a control in a total volume of 200 μl.

[Multiple insecticide resistances and mutation of in north-central Anhui Province].

Objective: To understand the status of resistance to multiple insecticides and the frequencies of mutations in from north-central Anhui Province.

Methods: From July to September, 2014, the mosquito larvae were collected in Huaibei, Bengbu and Chuzhou cities of the north-central Anhui Province and reared to adults. The female adult mosquitoes at 3-5 days post emergence were tested for susceptibility to the four insecticides, namely 0.

[The Role of Adoptive Transfer of Immune Cells in Helminth- induced Regulation of Allergy and Autoimmune Diseases].

Parasitic worms (helminth) or their derivates can inhibit allergy and autoimmune diseases by inducing the activation of immune cells and thus the release of regulatory factors. A large number of animal experiments have shown that adoptive transfer of lymphocytes can protect against immune deregulation and have potential clinical applications. In this review, we discuss the research progress on the role of adoptive transfer of immune cells in worm-induced regulation of allergy and autoimmune diseases.