Characterization of fatty acid desaturase gene family in and their expression patterns in seeds after infection.

Background: The family of membrane-bound fatty acid desaturase () genes play a vital role in plant growth, development, and stress responses. The seed-borne pathogen causes seed decay disease during pre-harvest and post-harvest stages of soybean, leading to a significant reduction in yield and quality. Therefore, it is very meaningful to characterize the diversity and function of the gene family in soybean and to elucidate their roles in seed resistance to

Results: In this study, 30 full-length genes were identified from the soybean genome.

Management of children and adolescents with chronic myeloid leukemia in chronic phase: International pediatric chronic myeloid leukemia expert panel recommendations.

The treatment strategy for children and adolescents with chronic myeloid leukemia in the chronic phase (CML-CP) has evolved from allogeneic hematopoietic stem cell transplantation (HSCT) to tyrosine kinase inhibitors (TKIs). With the advent of next-generation TKIs and new targeted therapies in the CML field, an international pediatric CML expert panel provides recommendations based on the medical literature (including previous pediatric guidelines), national standards, and treatment principles used in adults with CML-CP. Recommendations include diagnosis of the disease and details on managing the initial steps of care of children and adolescents with newly diagnosed CML-CP, including complications such as leukostasis.

Trans-zeatin modulates shade stress adaptation in soybean through transcription associated metabolic network.

This study explores the molecular mechanisms by which trans-zeatin (tZ), a cytokinin, influences shade stress responses in shade-sensitive and shade-tolerant recombinant inbred lines (RILs) 160 and 165 of soybean (Glycine max) under varied light conditions. Using an integrative multi-omics approach combining metabolomics and transcriptomics, we elucidate the regulatory networks underlying soybean adaptation to shade stress. Using an integrative multi-omics approach that combines metabolomics and transcriptomics, we dissect the complex regulatory networks that enable soybean plants to adapt to shade stress.

[Clinical and Laboratory Characteristics of Streptococcus mitis Causing Bloodstream Infection in Children with Hematological Disease].

Objective: To investigate the risk factors, clinical characteristics, and bacterial resistance of bloodstream infections caused by in children with hematological disease, so as to provide a reference for infection control.

Methods: The clinical information and laboratory findings of pediatric patients complicated with blood cultures positive for from January 2018 to December 2020 in the Institute of Hematology & Blood Diseases Hospital were searched and collected. The clinical characteristics, susceptibility factors, and antibiotic resistance of the children were retrospectively analyzed.

Pyruvate kinase M2 modulates mitochondrial dynamics and EMT in alveolar epithelial cells during sepsis-associated pulmonary fibrosis.

Background: Pulmonary fibrosis (PF) severely impacts both the survival and quality of life of patients with acute respiratory distress syndrome (ARDS) and remains a leading cause of late-stage ARDS-related mortality. The role of epithelial-mesenchymal transition (EMT) in alveolar epithelial cells (AECs) is pivotal in the development of PF.

Methods: This study explored the modulation of mitochondrial dynamics and the induction of EMT by pyruvate kinase M2 (PKM2) in AECs, aiming to identify new strategies for the prevention and treatment of sepsis-associated PF.

Screening of soybean antifungal isoflavones based on targeted metabolomics analysis.

Soybean ( max) is a key crop rich in bioactive compounds, particularly isoflavones, which play a significant role in plant defense against biotic stress like fungal infections. In this study, nine soybean varieties with varying susceptibility to mildew were evaluated. Isoflavone profiles in seeds and pods were analyzed using high-performance liquid chromatography-mass spectrometry (HPLC-MS), and metabolomics analysis via orthogonal signal correction partial least squares discriminant analysis (OSC-PLS-DA) identified differences between mildew-affected and unaffected samples.

Population pharmacokinetics and pulmonary modeling of eravacycline and the determination of microbiological breakpoint and cutoff of PK/PD.

Eravacycline is a broad-spectrum fluorocycline currently approved for complicated intra-abdominal infections (cIAIs). In lung-infection models, it is effective against methicillin-resistant (MRSA) and tetracycline-resistant MRSA. As such, we aimed to develop a population pharmacokinetic/pharmacodynamic (PK/PD) model to evaluate eravacycline's pulmonary distribution and kinetics.

A Zn-doped SbTe flexible thin film with decoupled Seebeck coefficient and electrical conductivity band engineering.

SbTe-based flexible thin films can be utilized in the fabrication of self-powered wearable devices due to their huge potential in thermoelectric performance. Although doping can significantly enhance the power factor value, the process of identifying suitable dopants is typically accompanied by numerous repeating experiments. Herein, we introduce Zn doping into thermally diffused p-type SbTe flexible thin films with a candidate dopant validated using the first-principles calculations.

Cianidanol from Sea Buckthorn Exert Anti-Inflammatory Effects by the Inhibiting JAK2/STAT3 Signaling Pathway via an Integrative Pharmacology Strategy.

Sea buckthorn is a model of medicine and food homology, but the chemical composition and mechanism of anti-inflammatory effects are limited. In this study, the key components and mechanisms of the anti-inflammatory effects of sea buckthorn were identified based on UPLC-Q-TOF-MS, network pharmacology, molecular docking, molecular dynamics and RAW264.7 cells.

Shading stress promotes lignin biosynthesis in soybean seed coat and consequently extends seed longevity.

The macromolecular components of the seed coat, particularly lignin, play a critical role in regulating seed viability. In the maize-soybean intercropping (MSI) system, shading stress was reported to enhance the viability of soybean seeds. However, the specific role of seed coat lignin in this process remains poorly understood.

Relay intercropped soybean promotes nodules development and nitrogen fixation by root exudates deposition.

Background: Legumes, in the initial event of symbiosis, secrete flavonoids into the rhizosphere to attract rhizobia. This study was conducted to investigate the relationship between crop root exudates and soybean nodule development under intercropping patterns.

Method: A two years field experiments was carried out and combined with pot experiments to quantify the effects of planting mode, i.

Single-cell epigenetic and clonal analysis decodes disease progression in pediatric acute myeloid leukemia.

Pediatric acute myeloid leukemia (pAML) is a clonal disease with recurrent genetic alterations that affect epigenetic states. However, the implications of epigenetic dysregulation in disease progression remain unclear. Here, we interrogated single-cell and clonal level chromatin accessibility of bone marrow samples from 28 patients with pAML representing multiple subtypes using mitochondrial single-cell assay for transposase-accessible chromatin with sequencing, which revealed distinct differentiation hierarchies and abnormal chromatin accessibility in a subtype-specific manner.

Emerging role of rare earth elements in biomolecular functions.

The importance of rare earth elements is increasingly recognized due to the increased demand for their mining and separation. This demand is driving research on the biology of rare earth elements. Biomolecules associated with rare earth elements include rare earth element-dependent enzymes (methanol dehydrogenase XoxF, ethanol dehydrogenase ExaF/PedH), rare earth element-binding proteins, and the relevant metallophores.

Multi-view BLUP: a promising solution for post-omics data integrative prediction.

Phenotypic prediction is a promising strategy for accelerating plant breeding. Data from multiple sources (called multi-view data) can provide complementary information to characterize a biological object from various aspects. By integrating multi-view information into phenotypic prediction, a multi-view best linear unbiased prediction (MVBLUP) method is proposed in this paper.

The impact of SARS-CoV-2 infection on immunity reconstitution among pediatric patients after allogeneic hematopoietic stem cell transplantation: a propensity score-matched analysis.

Background: Immunity reconstitution (IR) is crucial for pediatric patients undergoing hematopoietic stem cell transplantation (HSCT), but the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on lymphocyte subsets post-transplant remains unclear. Therefore, we assessed immune cell dynamics in children after SARS-CoV-2 infection.

Methods: We enrolled 42 children, including 21 post-HSCT SARS-CoV-2 infected and 21 matched, non-infected historical controls (1:1 matching based on propensity scores).

Emergy-based evaluation of production efficiency and sustainability of diversified multi-cropping systems in the Yangtze River Basin.

Excessive agricultural investment brought about by increased multiple-cropping index may compromise environmental sustainability. There are few studies on the sustainability of diversified multi-cropping systems in the Yangtze River Basin (YRB). Therefore, this study selected five representative locations in the YRB.

A metabolic roadmap of waxy corn flavor.

As well as being a popular vegetable crop worldwide, waxy corn represents an important amylopectin source, but little is known about its breeding history and flavor characteristics. In this study, through comparative-omic analyses between 318 diverse waxy corn and 507 representative field corn inbred lines we revealed that many metabolic pathways and genes exhibited selection characteristics during the breeding history of waxy corn, contributing to the divergence between waxy and field corn. We showed that waxy corn is not only altered in its glutinous property but also its sweetness, aroma, and palatability are all significantly affected.

Simulation of Defoliation Effects on Relay Strip Intercropping Soybean: Elucidating Foliar Shedding and Leaf-to-Nodule Growth Plasticity.

Extensive foliar shedding in monoculture soybeans post-anthesis negatively impacts yield, whereas relay strip intercropping prolongs leaf area duration, enhancing productivity. However, little is known about the causes of leaf shedding in monoculture and its impact on physiological functions and plasticity of source and sink organs, we conducted a 4-year field experiment and leaf-removal simulations in relay intercropped soybeans. Results revealed that monoculture soybeans experienced severe self-shading and defoliation, while relay intercropping maintained better light conditions, supporting higher leaf area, nodule numbers, and carbon allocation.

Far-red light mediates light energy capture and distribution in soybeans (Glycine max L.) under the shade.

Shade avoidance syndrome (SAS) is an important measure of how plants respond to shade. Shade conditions are characterized by low light density and low red-to-far-red light (R/FR) ratio. However, low light intensity and low R/FR ratio play considerable and diverse roles in SAS.

Defining a Therapeutic Target for Ganciclovir Therapy in Immunocompromised Children With Cytomegalovirus Infection: A Brief Report.

Ganciclovir and valganciclovir are first-line treatments for cytomegalovirus in immunocompromised children; however, the optimal therapeutic target remains unclear. This review identified 6 studies that showed clearance of cytomegalovirus viremia occurs with a median area under the concentration-time curve (AUC 24 ) between 23 and 70 μg·h/mL, with no clear correlation with efficacy or toxicity.

Relay strip intercropping of soybeans and maize achieves high net ecosystem economic benefits by boosting land output and alleviating greenhouse gas emissions.

Background: Cereal-legume intercropping provides a solution for achieving global food security, but the mechanism of greenhouse gas emissions and net ecosystem economic benefits of maize-soybean relay intercropping are poorly understood. Hence, we conducted a two-factor experiment to investigate the effects of cropping systems, containing maize-soybean relay intercropping (IMS), monoculture maize (M) and monoculture soybean (S), as well as three nitrogen levels at 0 (N0), 180 (N1), 240 (N2) kg N ha on crop grain yield, greenhouse gas emissions, soil carbon stock and net ecosystem economic benefit (NEEB).

Results: The average grain yield of IMS (7.

Clinical data and MRI features-based nomogram for differentiation of central nervous system infection and central nervous system involvement in hematological malignancy.

Central nervous system leukemia (CNSL) and central nervous system infection (CNSI) are the most important complications in patients with acute leukemia (AL). However, the differential diagnosis could represent a major challenge since the two disorders are all heterogeneous entities with overlapping clinical characteristics and radiological appearances. In this paper, we conduct a retrospective study to develop a model based on clinical data and magnetic resonance imaging (MRI) to distinguish CNSL from CNSI.