Identification of circRNAs and circRNA-mRNA network of Epinephelus coioides during Singapore grouper iridovirus infection.

Circular RNA (circRNA), one of the important non-coding RNA molecules with a closed-loop structure, plays a key regulatory role in cell processing. In this study, circRNAs of Epinephelus coioides, an important marine cultured fish in China, were isolated and characterized, and the network of circRNAs and mRNA was explored during Singapore grouper iridovirus (SGIV) infection, one of the most important double stranded DNA virus pathogens of marine fish. 10 g of raw data was obtained by high-throughput sequencing, and 2599 circRNAs were classified.

Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis.

Rubber tree is a major commercial source of natural rubber. Latex coagulation is delayed by thiols, which belong to the important type of antioxidants in laticifer submembrane, and is composed of glutathione (GSH), cysteine, and methionine. The rate-limiting enzyme, γ-ECS, plays an important role in regulating the biosynthesis of glutathione under any environment conditions.

iASPP is important for bladder cancer cell proliferation.

Inhibitor of apoptosis stimulatory protein phosphatase (iASPP) is a key inhibitor of p53 conserved from worm to human and is associated with cell proliferation and carcinogenesis in a variety of human cancers. Because iASPP is important for tumor cell apoptosis, it is a potential target for cancer gene therapy. However, it is still not clear whether iASPP is relevant to p53-deficient human bladder cancer.

[Value of 18F-FETNIM PET-CT for detection of tumor hypoxia in non-small-cell lung cancer].

Objective: To assess the feasibility of [(18)F]fluoroerythronitroimidazole ((18)F-FETNIM) with integrated positron emission tomography and computed tomography (PET-CT) imaging in detection of hypoxia in non-small-cell lung cancer (NSCLC) patients.

Methods: Forty-two patients with newly diagnosed NSCLC underwent (18)F-FETNIM PET-CT before treatment. Nineteen patients rested for approximately 120 minutes before undergoing PET-CT, 23 patients underwent 2 sequential PET-CT scans at 60 minutes and 120 minutes after intravenous injection (18)F-FETNIM.

[Evaluation of the diagnostic value of (18)F-FDG PET-CT and enhanced CT for staging of lymph node metastasis in non-small cell lung cancer].

Objective: To evaluate the clinical value of (18)F-FDG PET-CT and enhanced CT imaging for staging of regional lymph node metastasis in non-small cell lung cancer (NSCLC) patients.

Methods: 122 patients with proven or suspected NSCLC underwent integrated PET-CT and enhanced CT scan before surgery. The results of lymph node metastasis diagnosed by PET-CT and CT were compared and analyzed according to the results of histopathological examination.

[Correlation between FDG PET /CT and the expression of glutl and ki-67 antigen in esophageal cancer].

Objective: To evaluate the correlation between standardized uptake valus (SUV) of 18F-fluorodeoxyglucose (18 F-FDG) of tumor at PET/CT examination and the expression of glucose transporter-1 (Glutl) and Ki-67 in esophageal cancer.

Methods: 56 patients with esophageal cancer were evaluated with 18 F-FDG PET/CT examination before operation. The expression of Glut1 and Ki-67 antigen in the tumor tissues was detected by immunohistochemistry after operation.

[Expression of CD80, CD86, TGF-beta1 and IL-10 mRNA in the esophageal carcinoma].

Objective: To investigate the correlation of CD80 and CD86 mRNA expression with the expression of transforming growth factor-beta1 mRNA (TGF-beta1) and interleukin-10 mRNA (IL-10) in the esophageal cancer. To explore the reason of impaired immunological function of dentritic cell (DC) and the mechanism of cancer cell escaption from body immunity system in the esophageal cancer patient.

Methods: Expression of CD80, CD86, TGF-beta1 and IL-10R mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in specimens of 62 esophageal carcinoma and 16 normal esophageal mucosal tissues used as normal control.

[The effects of vascular endothelial growth factor on dendritic cells in esophageal tumor tissue].

Aim: To investigate the relationship between vascular endothelial growth factor (VEGF) and S100 protein positive dendritic cell (DC) in esophagelal tumor tissue.

Methods: VEGF and S100 protein in 94 patients with esophageal carcinoma were detected by HRP Labelled streptavidin biotin(LSAB) immunohistochemical method.

Results: Postive rate of VEGF in esophageal tumor tissues was 74.

[Expression of CD1a, CD80, CD86 in dendritic cell of tumor tissue and regional lymph node in esophageal carcinoma].

Background & Objective: Dendritic cells are the important antigen presenting cell, and take part in the anti-tumor immunoreaction. This study was designed to investigate the expression of CD1a (specific marker) and CD80, CD86 (costimulating molecules) in dendritic cell of tumor tissue and regional lymph node of esophageal carcinoma and to investigate the mechanisms by which tumors escape immune recognition.

Methods: The expression of CD1a, CD80, CD86 in dendritic cells of tumor tissues and paratumor tissues from 58 patients with esophageal carcinoma (clinical period I-II:27 cases,III-IV: 31 cases;differentiation G(1-2): 37 cases,G3:21 cases;squamous cancer: 49 cases,adenoid cancer: 9 cases;lymph node metastases present: 37 cases,lymph node metastases absent: 21 cases), inflammatory tissues from 11 patients with esophagitis, 12 normal esophageal tissues (as control), 31 regional lymph nodes with tumor and 34 regional lymph nodes without tumor were determined using flow cytometry.