Homocysteine affects macrophage polarization by altering m6A methylation of scavenger receptors CD209 and CD163L1.

Atherosclerosis is a chronic inflammatory disease characterized by fatty plaque deposits on artery walls. Elevated plasma homocysteine (Hcy) levels are an independent risk factor for atherosclerosis. Research on the mechanism by which Hcy promotes atherosclerosis has gradually turned to epigenetic inheritance, but the correlation between Hcy and m6A (N6-methyladenosine) modification has not been reported.

Correction: Human milk oligosaccharides and milk fat globule membrane reduce allergic reactions in mice through the modulation of gut microbiota and metabolic functions.

Correction for 'Human milk oligosaccharides and milk fat globule membrane reduce allergic reactions in mice through the modulation of gut microbiota and metabolic functions' by Xinzhang Chen , , 2024, , 11252-11265, https://doi.org/10.1039/D4FO03851G.

Research of recombinant influenza A virus as a vector for Mycoplasma pneumoniae P1a and P30a.

Background: Mycoplasma pneumoniae (MP) is a common respiratory pathogen affecting the longevity of the elderly and the health of children. However, the human vaccine against MP has not been successfully developed till now due to the poor immunogenicity and side effects of MP inactivated or attenuated vaccine. Therefore, it is necessary to develop a MP genetic engineering vaccine with influenza virus strain as vector.

Effects of single unilaterally impacted mesiodens on maxillary central incisors: A 3D quantitative assessment based on cone-beam computed tomography.

Background: Mesiodens usually lead to the malposition and abnormal morphology of maxillary central incisors.

Aim: To evaluate the detrimental effects of single unilaterally impacted mesiodens on the three-dimensional positions and morphology of the maxillary central incisor using cone-beam computed tomographic (CBCT) examinations.

Design: A total of 321 patients aged 5-17 years with single unilaterally impacted mesiodens were included and divided into two groups: mixed dentition group (5-10 years) and early permanent dentition group (11-17 years).

Fully automated deep learning model for detecting proximity of mandibular third molar root to inferior alveolar canal using panoramic radiographs.

Objective: This study endeavored to develop a novel, fully automated deep-learning model to determine the topographic relationship between mandibular third molar (MM3) roots and the inferior alveolar canal (IAC) using panoramic radiographs (PRs).

Study Design: A total of 1570 eligible subjects with MM3s who had paired PR and cone beam computed tomography (CBCT) from January 2019 to December 2020 were retrospectively collected and randomly grouped into training (80%), validation (10%), and testing (10%) cohorts. The spatial relationship of MM3/IAC was assessed by CBCT and set as the ground truth.

Impact of shale gas wastewater discharge on the trace elements of the receiving river in the Sichuan Basin, China.

The potential contamination of shale gas wastewater generated from hydraulic fracturing to water resources is of growing concern, yet minimum attention has been paid to the impact of shale gas wastewater on the trace elements of the receiving waters. In this study, we analyzed the levels of 50 trace elements of a river that receives effluent from a shale gas wastewater treatment facility in the Sichuan Basin, China. Sixteen trace elements were detected in the surface water sample from the effluent discharge site, all of which were of higher concentrations than the upstream background level.

Simple and active magnetic-field stabilization for cold atom experiments.

Cold atom experiments usually need a controllable and low-noise bias magnetic field to provide a quantization axis. Most labs need home-made stabilization of the field according to the actual setup, as commercially available power supply cannot directly satisfy their requirements. Here, by measuring the field fluctuations and active feedback modulating current supply of the applied magnetic field, we successfully demonstrate a field of 10.

Detection and Quantitation of Adulterated Paprika Samples Using Second-Order HPLC-FLD Fingerprints and Chemometrics.

Paprika is a widely consumed spice in the world and its authentication has gained interest considering the increase in adulteration cases in recent years. In this study, second-order fingerprints acquired by liquid chromatography with fluorescence detection (HPLC-FLD) were first used to detect and quantify adulteration levels of Chinese paprika samples. Six different adulteration cases, involving paprika production region, cultivar, or both, were investigated by pairs.

Testing Real Quantum Theory in an Optical Quantum Network.

Quantum theory is commonly formulated in complex Hilbert spaces. However, the question of whether complex numbers need to be given a fundamental role in the theory has been debated since its pioneering days. Recently it has been shown that tests in the spirit of a Bell inequality can reveal quantum predictions in entanglement swapping scenarios that cannot be modeled by the natural real-number analog of standard quantum theory.

Parahydrogen-Induced Polarization in Hydrogenation Reactions Mediated by a Metal-Free Catalyst.

We report nuclear spin hyperpolarization of various alkenes achieved in alkyne hydrogenations with parahydrogen over a metal-free hydroborane catalyst (HCAT). Being an intramolecular frustrated Lewis pair aminoborane, HCAT utilizes a non-pairwise mechanism of H transfer to alkynes that normally prevents parahydrogen-induced polarization (PHIP) from being observed. Nevertheless, the specific spin dynamics in catalytic intermediates leads to the hyperpolarization of predominantly one hydrogen in alkene.

Bimodal Fluorescence/Magnetic Resonance Molecular Probes with Extended Spin Lifetimes.

Bimodal molecular probes combining nuclear magnetic resonance (NMR) and fluorescence have been widely studied in basic science, as well as clinical research. The investigation of spin phenomena holds promise to broaden the scope of available probes allowing deeper insights into physiological processes. Herein, a class of molecules with a bimodal character with respect to fluorescence and nuclear spin singlet states is introduced.

Exotic nuclear spin behavior in dendritic macromolecules.

Dendrimers are a class of branched, highly symmetric macromolecules that have been shown to be useful for a vast number of different applications. Potential uses as fluorescence sensors, in catalysis and perhaps most importantly in medical applications as drug delivery systems or cytotoxica have been proposed. Herein we report on an exotic behaviour of the nuclear spins in a dendritic macromolecule in the presence of different paramagnetic ions.

Cavity-Enhanced Atom-Photon Entanglement with Subsecond Lifetime.

A cold atomic ensemble suits well for optical quantum memories, and its entanglement with a single photon forms the building block for quantum networks that give promise for many revolutionary applications. Efficiency and lifetime are among the most important figures of merit for a memory. In this Letter, we report the realization of entanglement between an atomic ensemble and a single photon with subsecond lifetime and high efficiency.

E-cadherin Plays a Role in Hepatitis B Virus Entry Through Affecting Glycosylated Sodium-Taurocholate Cotransporting Polypeptide Distribution.

Hepatitis B virus (HBV) infection is a major cause of chronic liver disease and hepatocellular carcinoma. Current antiviral therapy does not effectively eradicate HBV and further investigations into the mechanisms of viral infection are needed to enable the development of new therapeutic agents. The sodium-taurocholate cotransporting polypeptide (NTCP) has been identified as a functional receptor for HBV entry in liver cells.

Nuclear Spin Singlet States in Photoactive Molecules: From Fluorescence/NMR Bimodality to a Bimolecular Switch for Spin Singlet States.

Nuclear spin singlet states are silent states in nuclear magnetic resonance (NMR). However, they can be probed indirectly and offer great potential for the development of contrast agents for magnetic resonance imaging (MRI). Introduced here are two novel concepts: Firstly, the bimodal NMR/fluorescence properties of C -tetraphenylethylene.

Magnetic-enhanced modulation transfer spectroscopy and laser locking for Rb repump transition.

Locking of a laser frequency to an atomic or molecular resonance line is a key technique in applications of laser spectroscopy and atomic metrology. Modulation transfer spectroscopy (MTS) provides an accurate and stable laser locking method which has been widely used. Normally, the frequency of the MTS signal would drift due to Zeeman shift of the atomic levels and rigorous shielding of stray magnetic field around the vapor cell is required for the accuracy and stability of laser locking.

Development of a near-infrared fluorescent sensor with a large Stokes shift for sensing pyrophosphate in living cells and animals.

The detection of pyrophosphate (PPi) in living systems has attracted considerable attention in recent years due to its biological significance. While many fluorescent PPi sensors were developed with emission changes in the visible region, near-infrared (NIR) fluorescent sensors for PPi are rather scarce. In this paper, a dicyanomethylene-benzopyran (DCMB) based phenol-bridged dinuclear Zn(II)-DPA (DPA: dipicolylamine) complex was prepared, which was found to be a promising NIR fluorescent sensor for PPi.

Pulsed Magnetic Resonance to Signal-Enhance Metabolites within Seconds by utilizing -Hydrogen.

Diseases such as Alzheimer's and cancer have been linked to metabolic dysfunctions, and further understanding of metabolic pathways raises hope to develop cures for such diseases. To broaden the knowledge of metabolisms in vitro and in vivo, methods are desirable for direct probing of metabolic function. Here, we are introducing a pulsed nuclear magnetic resonance (NMR) approach to generate hyperpolarized metabolites within seconds, which act as metabolism probes.

The expression and significance of tumor associated macrophages and CXCR4 in non-small cell lung cancer.

Purpose: The purpose of this investigation was to determine the expression and significance of tumor associated macrophages (TAMs) and CXCR4 in non-small cell lung cancer (NSCLC).

Methods: Immunohistochemical staining was used to analyze the expression of CD68 (TAM surface marker) and CXCR4 in 68 cases of NSCLC and 17 cases of normal lung tissue.

Results And Conclusion: The positive rate of CD68 was 66.

Detection and differentiation of Cys, Hcy and GSH mixtures by F NMR probe.

Simultaneous detection and differentiation of biomolecules is of significance in biological research. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. However, existing methods for simultaneous detection and differentiation of Cys, Hcy, and GSH are still challenging because of their similarity in structure and chemical properties.

Hyperpolarized Xe Magnetic Resonance Imaging Sensor for H S.

A new magnetic resonance imaging (MRI) molecular sensor for hydrogen sulfide detection and imaging using the nuclear spin resonance of hyperpolarized Xe is developed. The designed MRI sensor employs cryptophane for NMR sensing, together with an azide group serving as a reaction site. It demonstrates a "proof-of-concept" that a fluorescent H S probe can be linked to a xenon-binding cryptophane and thereby converted into an MRI probe, which could provide a very generalizable template.

Hong-Ou-Mandel Interference between Two Deterministic Collective Excitations in an Atomic Ensemble.

We demonstrate deterministic generation of two distinct collective excitations in one atomic ensemble, and we realize the Hong-Ou-Mandel interference between them. Using Rydberg blockade we create single collective excitations in two different Zeeman levels, and we use stimulated Raman transitions to perform a beam-splitter operation between the excited atomic modes. By converting the atomic excitations into photons, the two-excitation interference is measured by photon coincidence detection with a visibility of 0.

Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction.

Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. Knowledge of their biodistribution in real-time could help diagnose a variety of conditions. However, existing methods of biothiol detection are invasive and require assays.

[NaHS inhibits A549 cell injury induced by lipopolysaccharide].

Objective: To observe the effect of NaHS on lipopolysaccharide (LPS)-induced injury of A549 lung cancer cells and explore the possible mechanisms.

Methods: A549 cells were divided into 4 groups: control group, 10 μg/mL LPS treated group, 10 μmol/L NaHS treated group and the group treated with 10 μg/mL LPS plus 10 μmol/L NaHS. After treatment for 24 hours, C-reactive protein (CRP) content in culture solution was measured by ELISA, and the cell viability was detected by MTT assay and lactate dehydrogenase (LDH) assay.