Novel Genetic Loci Associated with PhenoAge Acceleration - Changzhou City, Jiangsu Province, China, 2012-2019.

What Is Already Known About This Topic?: China is rapidly encountering population aging, yet studies on aging are limited by the traditional aging measure: chronological age, particularly in the field of genomics. Several promising aging measures have been proposed, but they lack comparative evaluation.

What Is Added By This Report?: PhenoAge was identified as a measure of aging that demonstrated greater applicability in contemporary populations.

Development and immunogenicity evaluation of attenuated Salmonella typhimurium delivering porcine Deltacoronavirus S1 gene.

Porcine deltacoronavirus (PDCoV) is a swine enteropathogenic coronavirus that causes severe diarrhea in piglets, the development of novel vaccines is of great value in the prevention and control of PDCoV. Here, we selected attenuated Salmonella typhimurium SL7207 to deliver pVAX1-S1, resulting in the oral vaccine strain, SL7207 (pVAX1-S1). In immunized mice, SL7207 (pVAX1-S1) induced PDCoV-specific humoral IgG, IgA, neutralizing antibodies, mucosal sIgA, up-regulation of CD8 T cells, and increased levels of Th1 cytokines (IFN-γ and IL-2).

TMSN Initiated Electrochemical Mono-Dealkylation of Tertiary Amides.

-Dealkylation of amides is a general process in living organisms and organic synthetic chemistry, but an efficient chemical approach for this transformation has not been explored. Herein, we report an electrochemical method for the monodealkylation of a wide range of tertiary amides, including benzamides, alkyl amides, lactams, and sulfonamides. The reaction proceeds smoothly under mild conditions using TMSN as the initiator and is not limited to deethylation or demethylation.

Accelerating quad Airy beams-based point response for interferenceless coded aperture correlation holography.

Interferenceless-coded aperture correlation holography (I-COACH) is a promising single-shot 3D imaging method in which a coded phase mask (CPM) is used to encode 3D information about an object into an intensity distribution. However, conventional CPM encoding methods usually lead to intensity dilution, especially in the recording of point spread holograms (PSHs), resulting in low-resolution reconstruction of I-COACH. Here, we propose accelerating quad Airy beams with four mainlobes as a point response to enable weak diffraction propagation and a sharp maximum intensity in the transverse direction.

Efficient synthesis of α-amino-vinylphosphine oxides from alkyl nitriles manganese-catalyzed phosphinoenamination.

A protocol for the synthesis of α-amino-vinylphosphine oxides by phosphinoenamination reaction between alkyl nitriles and phosphine oxides was developed. The combination of Mn(OAc) as a Lewis acid and guanidine as a Lewis base was found to be an efficient catalytic system for this reaction. A series of alkyl nitriles and phosphine oxides are compatible with this conversion, furnishing the desired products in up to 95% yield under mild conditions.

Guild-level signature of gut microbiome for diabetic kidney disease.

Unlabelled: Current microbiome signatures for chronic diseases such as diabetic kidney disease (DKD) are mainly based on low-resolution taxa such as genus or phyla and are often inconsistent among studies. In microbial ecosystems, bacterial functions are strain specific, and taxonomically different bacteria tend to form co-abundance functional groups called guilds. Here, we identified guild-level signatures for DKD by performing in-depth metagenomic sequencing and conducting genome-centric and guild-based analysis on fecal samples from 116 DKD patients and 91 healthy subjects.

Alteration in gut microbiota is associated with immune imbalance in Graves' disease.

Background: Graves' disease (GD), characterized by immune aberration, is associated with gut dysbiosis. Despite the growing interest, substantial evidence detailing the precise impact of gut microbiota on GD's autoimmune processes remains exceedingly rare.

Objective: This study was designed to investigate the influence of gut microbiota on immune dysregulation in GD.

Identification of potential SLA-I-specific T-cell epitopes within the structural proteins of porcine deltacoronavirus.

Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that mainly threatens newborn piglets and poses a potential broad cross-species transmission risk. The antigenic epitopes of PDCoV are currently unidentified, and no information about T cell epitopes is available. Here, T-cell epitopes of PDCoV structural proteins were predicted using computational methods.

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes.

The pathogenic mechanisms underlying distal symmetric polyneuropathy (DSPN), a common neuropathy in patients with diabetes mellitus (DM), are not fully understood. Here, we discover that the gut microbiota from patients with DSPN can induce a phenotype exhibiting more severe peripheral neuropathy in db/db mice. In a randomized, double-blind, and placebo-controlled trial (ChiCTR1800017257), compared to 10 patients who received placebo, DSPN was significantly alleviated in the 22 patients who received fecal microbiota transplants from healthy donors, independent of glycemic control.

Effects of comfort nursing on pain and quality of life in children undergoing tonsillectomy.

Objective: To observe the effect of comfort nursing on pain, quality of life, and nutritional status in children undergoing tonsillectomy.

Methods: In this retrospective study, a total of 114 children who underwent tonsillectomy in Chun'an Hospital of Traditional Chinese Medicine were divided into a research group and a control group according to the nursing methods, with 57 cases in each group. The control group received routine nursing care, and the research group received additional comfort nursing.

Arthroscopic-assisted reduction plus internal fixation and traditional open reduction plus internal fixation for talus fractures: a comparative study.

Objective: To explore the efficacy of arthroscopic-assisted reduction and internal fixation (ARIF) and traditional open reduction and internal fixation in the treatment of talus fractures.

Methods: This study retrospectively analyzed the clinical data of 92 patients with talus fractures admitted to our hospital. The patients were divided into a control group (treated with traditional open reduction and internal fixation) and a research group (with ARIF) with 46 cases in each.

Cardiovascular Benefits of Empagliflozin Are Associated With Gut Microbiota and Plasma Metabolites in Type 2 Diabetes.

Context: Cardiovascular benefits of empagliflozin in patients with type 2 diabetes mellitus (T2DM) have been reported; however, the underlying mechanism remains unknown.

Objective: We hypothesized that the cardiovascular benefits of empagliflozin are associated with altered gut microbiota and plasma metabolites, and that empagliflozin may be used as an initial treatment for patients with T2DM at risk of cardiovascular diseases (CVDs).

Methods: This randomized, open-label, 3-month, 2-arm clinical trial included 76 treatment-naïve patients with T2DM and risk factors for CVD who were treated with either empagliflozin (10 mg/d, n = 40) or metformin (1700 mg/d, n = 36).

Aberrant expression of inhibitory receptors on B cells in patients with Graves' disease.

The pathophysiological mechanism underlying Graves' disease (GD) remains incompletely understood. Inhibitory receptors on B cells are critical for humoral immunity, which plays a key role in GD pathogenesis. This study aimed to investigate B cell subsets distribution and inhibitory receptor expression on these subsets in GD patients.

Characteristics of the Gut Microbiota and Metabolism in Patients With Latent Autoimmune Diabetes in Adults: A Case-Control Study.

Objective: Type 1 and type 2 diabetes are associated with gut dysbiosis. However, the relationship between the gut microbiota and latent autoimmune diabetes in adults (LADA), sharing clinical and metabolic features with classic type 1 and type 2 diabetes, remains unclear. Here, we used a multiomics approach to identify the characteristics of the gut microbiota and metabolic profiles in patients with LADA.

[Effects of multifunctional plant rhizosphere promoting bacteria on maize growth in black soil areas in Northeast China].

The application of microbial fertilizer plays an important role in improving soil restoration and fertilizer utilization. The effects of microbial fertilizer are greatly affected by crop genotypes and ecological conditions. Little is known about the effects of microbial fertilizers on maize production in Northeast China.

Investigation on the micromorphology and thermophysical properties of NaNO heat storage materials modified by solution combustion.

Nitrate has a wide temperature range, wide operating temperature, low vapor pressure, low cost, strong heat transfer and stable chemical properties. It is widely used in solar thermal power generation heat storage material. In this paper, the alkali salt NaNO was modified by solution combustion method with citric acid as fuel.

Association of serum CTRP9 levels with cardiac autonomic neuropathy in patients with type 2 diabetes mellitus.

Aims: Cardiac autonomic neuropathy (CAN) is a serious complication of diabetes and is associated with adipokines. The C1q tumor necrosis factor-related protein 9 (CTRP9) is a newly discovered adipokine. This study aimed to evaluate the association of serum CTRP9 levels with the prevalence and severity of CAN in patients with type 2 diabetes mellitus.

Association of time in range, as assessed by continuous glucose monitoring, with painful diabetic polyneuropathy.

Aims/introduction: This study aimed to evaluate the association between time in range (TIR) obtained from continuous glucose monitoring and the prevalence and degree of painful diabetic neuropathy.

Materials And Methods: A total of 364 individuals with diabetic peripheral neuropathy were enrolled in this study. Sensor-based flash glucose monitoring systems were used to monitor the participants' glucose levels, and the glycemic variability metrics were calculated, including the TIR, glucose coefficient of variation, standard deviation and the mean amplitude of glycemic excursions.

sp. nov., isolated from deep-sea sediment of the Pacific Ocean.

A taxonomic study was carried out on strain PA15-N-34, which was isolated from deep-sea sediment of Pacific Ocean. The bacterium was Gram-stain-positive, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinity of 0-15.

Association of masked uncontrolled hypertension and cardiovascular diseases in treated hypertensive patients.

Introduction: The aim of the study was to evaluate the association of masked uncontrolled hypertension (MUCH) and prevalence of cardiovascular disease in treated hypertensive patients.

Material And Methods: Patients' demographics and prior medical histories were collected. Fasting venous blood was drawn for evaluation of serum creatinine level, which was used to calculate glomerular filtration rate (GFR).

[Analysis of CYP17A1 gene variants in 5 patients with 17-hydroxylase deficiency].

Objective: To identify pathogenic variants in 5 sporadic patients and two Chinese pedigrees affected with 17-hydroxylase deficiency (17-OHD).

Methods: Peripheral blood samples were collected with informed consent. Variants of CYP17A1 gene were screened by PCR and Sanger sequencing.

Chengkuizengella marina sp. nov., isolated from deep-sea sediment of the Pacific Ocean.

A taxonomic study was carried out on strain YPA3-1-1, which was isolated from deep-sea sediment of the Pacific Ocean. The bacterium was Gram-stain-positive, oxidase-positive, catalase-negative, rod-shaped and spore-forming. Growth was observed at salinities of 1.

Integrated analysis of transcriptome data revealed MMP3 and MMP13 as critical genes in anaplastic thyroid cancer progression.

To better understand the molecular mechanisms of anaplastic thyroid carcinoma (ATC), we aimed to identify the hub genes specifically involved in ATC by integrated bioinformatics analysis. In this study, using three Gene Expression Omnibus data sets with the same platform GPL570, we screened hub genes involved in ATC progression. In vitro experiments, such as western blot analysis, Transwell assays, and coimmunoprecipitation, was performed to verify our findings.