Yield increment and transcriptome response caused by blue light treatment in Hericium coralloides.

Background: Hericium coralloides is a traditional edible and medicinal mushroom. Light is a key factor in forming fruiting bodies of fungi; however, the effects of different light on the yield and morphogenesis of H. coralloides are still unknown.

Electrochemiluminescence immunoassay of cTnI with ruthenium-based metal covalent organic framework and dual DNAzymes cascade amplification strategy.

Currently, metal-organic frameworks (MOFs) with tunability and covalent organic frameworks (COFs) with high stability are promising nanomaterials for electrochemiluminescence (ECL), while Ru-based metal covalent organic frameworks (Ru-MCOFs) have rarely been reported. Herein, an ECL immunosensor based on a strong ECL-emitting Ru-MCOF was proposed for the sensitive detection of the cardiac troponin-I (cTnI). Imine-linked Ru-MCOF was prepared as an ECL emitter via solvothermal method using tris(4,4' -diamino-2,2' -bipyridine)ruthenium(II) (Ru(dbpy)32+) as a precursor.

Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China.

Background: Eucalyptus grandis, which was first comprehensively and systematically introduced to China in the 1980s, is one of the most important fast-growing tree species in the forestry industry. However, to date, no core collection has been selected from the germplasm resources of E. grandis based on growth and genetic relationship analysis.

Toripalimab plus chemotherapy for first line treatment of advanced non-small cell lung cancer (CHOICE-01): final OS and biomarker exploration of a randomized, double-blind, phase 3 trial.

A randomized double-blind phase 3 trial (CHOICE-01, NCT03856411) demonstrated that combining toripalimab with chemotherapy substantially improves progression-free survival (PFS) in advanced non-small cell lung cancer (NSCLC) patients without pretreatment. This study presents the prespecified final analysis of overall survival (OS) and biomarkers utilizing circulating tumor DNA (ctDNA) and tissue-based sequencing. Additionally, the analysis revealed a higher median overall survival (OS, 23.

Small Extracellular Vesicles Isolated from Cardiac Tissue Matrix or Plasma Display Distinct Aging-Related Changes in Cargo Contributing to Chronic Cardiovascular Disease.

Aging is a major risk factor for cardiovascular disease, the leading cause of death worldwide, and numerous other diseases, but the mechanisms of these aging-related effects remain elusive. Chronic changes in the microenvironment and paracrine signaling behaviors have been implicated, but remain understudied. Here, for the first time, we directly compare extracellular vesicles obtained from young and aged patients to identify therapeutic or disease-associated agents, and directly compare vesicles isolated from heart tissue matrix (TEVs) or plasma (PEVs).

Renin-independent aldosteronism and metabolic dysfunction-associated steatotic liver disease and cirrhosis: A genetic association study.

Background & Aims: Renin-independent aldosteronism (RIA) refers to a spectrum of autonomous aldosterone hypersecretion. We aimed to explore the genetical relationship between RIA and metabolic dysfunction-associated steatotic liver disease (MASLD) and cirrhosis.

Methods: We included 125357 participants from the cohort of United Kingdom Biobank.

A combined model of circulating tumor DNA methylated SHOX2/SCT/HOXA7 and clinical features facilitates the discrimination of malignant from benign pulmonary nodules.

Background: Despite the advancements in early lung cancer detection attributed to the widespread use of low-dose computed tomography (LDCT), this technology has also led to an increasing number of pulmonary nodules (PNs) of indeterminate significance being identified. Therefore, this study was aimed to develop a model that leverages plasma methylation biomarkers and clinical characteristics to distinguish between malignant and benign PNs.

Methods: In a training cohort of 210 patients with PNs, we evaluated plasma circulating tumor DNA (ctDNA) for the presence of three lung cancer-specific methylation markers: SHOX2, SCT, and HOXA7.

Roles of the Polymer Backbone for Sulfurized Polyacrylonitrile Cathodes in Rechargeable Lithium Batteries.

Sulfurized polyacrylonitrile (SPAN) has emerged as a highly promising cathode material for next-generation lithium-sulfur (Li-S) batteries primarily due to its non-polysulfide dissolution and excellent cycle stability. Nevertheless, the specific roles and impacts of the pyrolyzed polyacrylonitrile, which constitutes the polymer backbone of SPAN, remain inadequately understood. In this study, comprehensive investigations from multiple aspects, including electrochemistry, spectroscopy, electron microscopy, and theoretical calculations, were conducted on a series of SPAN materials with various sulfur contents.

Pembrolizumab versus sintilimab in patients with advanced NSCLC: a retrospective multicenter study with propensity-score matching analysis.

Background: Programmed cell death protein 1 (PD-1) inhibitors are commonly used worldwide for the management of non-small cell lung cancer (NSCLC). However, it remains unclear whether pembrolizumab and sintilimab, two of the most widely used PD-1 inhibitors in China, have significantly different effects on patients with NSCLC. A multicenter retrospective cohort study was designed and implemented using propensity-score matching (PSM) analysis to compare the effectiveness and safety profiles of pembrolizumab and sintilimab in patients with advanced NSCLC undergoing comprehensive therapy.

Treating early-stage centrally-located non-small cell lung cancer with DCAT-SBRT in centers lacking the VMAT technique: a comprehensive study.

Background: Volumetric-modulated arc therapy (VMAT) may have the highest overall performance for stereotactic body radiotherapy (SBRT) treatment of inoperable early-stage NSCLC. However, in centers lacking the VMAT technique, the dynamic conformal arc therapy (DCAT) technique is potentially the best option for small and rounded NSCLC-SBRT. Therefore, we will comprehensively analyze the advantages of the DCAT versus the other techniques except VMAT in terms of dosimetry, plan complexity, delivery time, γ-passing rates and the interplay effect.

Comprehensive analysis of skin growth-related hub genes and microenvironment characterization in a mouse expanded skin model.

Background: Mechanical stretch-mediated tissue expansion is effective for obtaining extra skin and soft tissue required for the repair of defects or reconstruction of surface organs. Understanding the cellular and molecular mechanisms and identifying hub genes and key cell types associated with skin expansion could help predict the success of skin growth during expansion procedures.

Methods: We analyzed murine chip sequencing data and single-cell sequencing data available from the Gene Expression Omnibus database.

CCT39 Transcription Factor Promotes Chlorophyll Biosynthesis and Photosynthesis in Poplar.

Chlorophyll serves as a crucial pigment in plants, essential for photosynthesis, growth, and development. Our previous study has shown that PpnCCT39 can increase leaf chlorophyll content and photosynthesis rate in poplar. However, the underlying molecular mechanisms remain unknown.

Behaviors and attitudes towards mobile learning during course work and scientific research among dental, medical and non-medical undergraduates: a cross-sectional study.

Background: Mobile learning is a valuable supplement to traditional education methods, but the current research on mobile learning is still insufficient. This study aimed to assess dental undergraduates' behaviors and attitudes towards mobile learning during course work and scientific research and to compare these findings to those of medical and non-medical students.

Methods: A cross-sectional survey was conducted among undergraduates at Sichuan University, China.

Combined inflammation-related biomarkers and clinicopathological features for the prognosis of stage II/III colorectal cancer by machine learning.

Background: Inflammation-related biomarkers, such as systemic inflammation score (SIS) and neutrophil-lymphocyte ratio (NLR), are associated with colorectal cancer prognosis. However, the combined role of SIS, NLR, and clinicopathological factors in stage II/III colorectal cancer remains unclear. This study developed a nomogram to predict long-term prognosis for these patients.

Evaluation of functional magnetic resonance APT and DKI imaging for breast cancer.

Objective: This study aimed to compare the performance of amide proton transfer-weighted imaging (APTWI) and diffusion kurtosis imaging (DKI) in differentiating benign from malignant breast lesions, evaluate molecular subtypes of breast cancer, and determine the diagnostic efficacy of the quantitative magnetic resonance imaging (qMRI) parameters in differentiating benign from malignant breast diseases.

Methods: The study included 168 women who underwent breast APTWI and DKI at Yunnan Cancer Hospital between December 2022 and July 2023. The APT signal intensity (SI), apparent kurtosis coefficient (Kapp), non-Gaussian diffusion coefficient (Dapp), and apparent diffusion coefficient (ADC) values were measured before surgery.

Liraglutide and GLP-1(9-37) alleviated hepatic ischemia-reperfusion injury by inhibiting ferroptosis via GSK3β/Nrf2 pathway and SMAD159/Hepcidin/FTH pathway.

Ferroptosis plays a pivotal role in the pathogenesis of ischemia-reperfusion injury (IRI). Liraglutide, as a GLP-1 receptor (GLP-1R) agonist, has exhibited extensive biological effects beyond its hypoglycemic action. Recent studies have shed light on the regulatory influence of Liraglutide on ferroptosis, yet the precise underlying mechanism remains elusive.

Treatment of blunt splenic injury in children in China.

Introduction: Nonoperative management (NOM) is recognized as a viable treatment for pediatric closed splenic trauma. However, clinical guidelines are applied inconsistently, resulting in different treatment strategies in different regions. This study aimed to investigate the independent risk factors influencing the length of stay in pediatric closed splenic injuries and to analyze the key determinants in the choice of surgical treatment to optimize inpatient management and patient care and improve outcomes.

A novel cyanine photosensitizer for sequential dual-site GSH depletion and ROS-potentiated cancer photodynamic therapy.

The efficacy of photodynamic therapy (PDT) is often limited by the reductive microenvironment in tumor cells due to the high level of glutathione (GSH) and glutathione peroxidase 4 (GPX4), which maintain redox homeostasis. Therefore, designing a GSH-responsive photosensitizer that depletes intracellular GSH is a promising strategy to enhance PDT selectivity and efficacy. Herein, we present a GSH-selective sequentially responsive theranostic photosensitizer, Cy-Res.

Clinical Actionability of the NUDT15 *4 (p.R139H) Allele and Its Association With Hispanic Ethnicity.

Nudix hydrolase 15 (NUDT15) deficiency is strongly associated with thiopurine-induced myelosuppression. Currently, testing for NUDT15 deficiency is based on the genotyping of the most frequent and clinically characterized no-function variants, that is, *2, *3 and *9. The Hispanic/Latino-predominant variant NUDT15 *4 (p.

Advanced Management of Hearing Loss: A Comprehensive Review of the Special Issue.

Hearing loss, affecting over 466 million people worldwide, poses significant challenges for affected individuals, their families, and healthcare providers alike [...

Investigation on a Novel Reinforcement Method of Grouting Sleeve Connection Considering the Absence of Reserved Reinforcing Bars in the Transition Layer.

In practical engineering, due to quality inspections of connections between prefabricated components and construction errors, reserved reinforcing bars in the transition layer may be partially insufficient or even completely absent. This defect significantly impacts the structural performance of sleeve connections, particularly under tensile or shear forces. This paper proposes a novel reinforcement method to address the connection issues caused by the absence of reserved reinforcing bars in the transition layer and verifies its feasibility through systematic experiments.

Cancer specific up-regulated lactate genes associated with immunotherapy resistance in a pan-cancer analysis.

Background: Although the lactate pathway has been reported to lead to immune escape through the inhibition of effector T cells, the cancer-intrinsic lactate signature has not been identified, and the immunotherapeutic efficacy and potential mechanism of the lactate signature are still unclear.

Methods: We defined a pan-cancer up-lactate score by comparing malignant tissues and normal tissues in the TCGA cohort. The immunotherapeutic efficacy was evaluated in non-small cell lung cancer (NSCLC), metastatic renal cancer (mRCC), bladder cancer (BLCA) and melanoma cohorts.

Invasive Group B Streptococcus Infections Caused by Hypervirulent Clone of S. agalactiae Sequence Type 283, Hong Kong, China, 2021.

During September-October 2021, group B Streptococcus bloodstream infections surged among patients hospitalized in Hong Kong. Of 95 cases, 57 were caused by the hypervirulent strain sequence type 283, which at the time was also found in freshwater fish and wet market environments and thus poses a transmission threat.

Paeonol inhibits ACSL4 to protect chondrocytes from ferroptosis and ameliorates osteoarthritis progression.

Background: Discovering an inhibitor for acyl-CoA synthetase long-chain family member 4 (ACSL4), a protein that triggers cell injury via ferroptosis, presents potential to minimize cellular damage. This study investigates paeonol (PAE), a traditional Chinese herbal medicine, as an ACSL4 inhibitor to prevent chondrocyte ferroptosis and protect against osteoarthritis (OA).

Methods: We conducted experiments using mouse chondrocytes treated with PAE to mitigate ferroptosis induced by Interleukin-1 Beta (IL-1β) or ferric ammonium citrate (FAC), examining intracellular ferroptotic indicators, cartilage catabolic markers, and ferroptosis regulatory proteins.