Zscan4 mediates ubiquitination and degradation of the corepressor complex to promote chromatin accessibility in 2C-like cells.

Zygotic genome activation occurs in two-cell (2C) embryos, and a 2C-like state is also activated in sporadic (~1%) naïve embryonic stem cells in mice. Elevated chromatin accessibility is critical for the 2C-like state to occur, yet the underlying molecular mechanisms remain elusive. Zscan4 exhibits burst expression in 2C embryos and 2C-like cells.

Genome Mining Reveals Biosynthesis of the Antifungal Lipopeptaibols, Texenomycins, through a Hybrid PKS-NRPS System, in the Fungus .

Texenomycins are a family of linear lipopeptaibols with a long polyketide side chain at the N-terminus and 21 amino acid residues at the -terminus, presenting demonstrated potential as antibiotics against plant fungal pathogens. In this study, texenomycins were identified and isolated from the fungus strain TTI-0396 and showed effective antifungal properties against two plant pathogens and . Through analysis of the whole-genome data of strain TTI-0396, we discovered a hybrid PKS-NRPS system with the polyketide synthase (PKS: TexQ), thioesterase (TexO), acyl-CoA ligase (TexI), and three nonribosomal peptide synthetases (NRPSs: TexG, TexJ, TexV) in the gene cluster that were proposed to be responsible for the biosynthesis of texenomycins and another related lipopeptaibol, lipohexin.

Microneedle-Based Electrochemical Array Patch for Ultra-Antifouling and Ultra-Anti-Interference Monitoring of Subcutaneous Oxygen.

Oxygen saturation is a crucial indicator in the management of various diseases and in preoperative diagnosis, and the detection of oxygen content is valuable in guiding clinical treatment. However, as the classical and dominant oxygen detection strategies, current photoelectric oximeters and electrochemical-based blood gas analyzers often suffer from significant interindividual variation and poor compliance, respectively. In recent years, wearable microneedles (MNs) for analyzing biomarkers in interstitial fluid (ISF) have received great attention and recognition mainly for the reason that the content of the substances distributed in ISF has a better correlation with that in blood circulation compared with other body fluids such as sweat and saliva.

Corrigendum: Mechanisms of mitophagy and oxidative stress in cerebral ischemia-reperfusion, vascular dementia, and Alzheimer's disease.

[This corrects the article DOI: 10.3389/fnmol.2024.

Antifungal activity of paeonol against by disrupting the cell membrane and the application on cherry tomato preservation.

may cause gray mold in fruits and vegetables. Paeonol, an active component of traditional Chinese medicine, could suppress various microbial growth. However, reports on its effect on have not yet been documented.

Multi-omics insights into beagle dog fed with a sucking-rewarded automatic feeding device.

Background: Facilitating the development of the sucking function in early stages of preterm infants holds substantial potential for influencing their long-term outcomes. To this end, our team has devised a sucking-rewarded automatic feeding device specifically tailored for preterm infants. The present study is designed to investigate the impacts of this innovative device, utilizing a multi-omics profiling approach, on beagle dogs as a surrogate model.

Meta-analysis and network pharmacology studies of the clinical efficacy of Guizhi Fuling capsules/pills combined with dienogest in treating endometriosis.

Background: Endometriosis (EMs) is a common chronic inflammatory gynecological disease that belongs to the classification of Traditional Chinese Medicine Syndromes "Zheng Jia," and the classic Chinese formula Guizhi Fuling (GZFL) demonstrates significant clinical efficacy in the treatment of this condition. This study aims to investigate GZFL's effect and potential mechanism in EMs.

Methods: The search reviewed randomized controlled trials in 7 databases from inception to 2024, assessed quality with the Cochrane tool, and analyzed data with STATA 15 by 2 reviewers.

Effect of extracorporeal shock wave therapy on nerve conduction: a systematic review and meta-analysis.

Background: Extracorporeal shock wave therapy (ESWT), as a non-invasive physical agent modality, was effective in relieving spasticity, reducing pain, and improving dysfunction. This systematic review and meta-analysis aimed to investigate the effect of ESWT on nerve conduction, and to find out whether the ESWT group is superior to the control or other comparison groups, thus providing support for guiding the rehabilitation of peripheral nerve injury in clinical work.

Methods: PubMed, Web of Science, the Cochrane Library, and Embase were searched from inception to August 20, 2024.

Therapeutic Efficacy of Tirofiban Combined With Thrombus Aspiration and Stent Thrombectomy in the Treatment of Large Vessel Occlusion Ischemic Stroke.

Objective: This research aimed to ascertain the effects of tirofiban combined with thrombus aspiration and stent thrombectomy on large vessel occlusion ischemic stroke (LVO-IS).

Methods: Sixty patients with acute ischemic stroke (AIS) caused by LVO were randomized into the control group and the intervention group (n=30). Patients in the control group received thrombus aspiration combined with stent thrombectomy, while those in the intervention group were treated with tirofiban combined with thrombus aspiration and stent thrombectomy.

Micro-nano hierarchical urchin-like ZnO/Ag hollow sphere for SERS detection and photodegradation of antibiotics.

Hollow urchin-like substrates have been widely interested in the field of surface-enhanced Raman scattering (SERS) and photocatalysis. However, most reported studies are simple nanoscale urchin-like substrate with limited light trapping range and complicated preparation process. In this paper, a simple and effective controllable synthesis strategy based on micro-nano hierarchical urchin-like ZnO/Ag hollow spheres was prepared.

Comprehensive analysis of the CPP gene family in Moso bamboo: insights into their role in rapid shoot growth.

Cysteine-rich polycomb-like proteins (CPPs), pivotal transcription factors crucial for evolution of plants from germination to maturity, and adaptation to environmental stresses, have not yet been characterized within the context of Moso bamboo. The CPP gene family of Moso bamboo was identified through bioinformatics, and the structural and functional attributes of the gene, including its physicochemical properties, evolutionary relationships, and gene-protein structures, were revealed. Additionally, the current study also offers valuable information on the patterns of gene expression in bamboo shoots during the period of accelerated development.

Integration of the systemic inflammatory response index with pulse pressure enhances prognostication of cardiovascular mortality in the general population of the United States: insights from the NHANES database.

Background: The prognostic significance of utilizing both the systemic inflammatory response index (SIRI) and pulse pressure (PP) collectively in assessing cardiovascular mortality (CVM) across populations remains to be elucidated.

Methods: Multivariate Cox proportional hazards analysis investigated the SIRI, PP, and CVM association. Receiver operating characteristic (ROC) curves evaluated the predictive performance of the combined SIRI and PP for CVM in the broader demographic.

Effect of Oat Hay as a Substitute for Alfalfa Hay on the Gut Microbiome and Metabolites of Yak Calves.

To evaluate the impact of different roughages on the intestinal microbiota of yak calves, we fed them oat hay in substitution of alfalfa hay, in addition to milk replacer and starter powder. Twenty-one 45-day-old male yak calves were selected and randomly assigned to three groups: the milk replacer + starter + alfalfa hay group (AH), the milk replacer + starter + oat hay group (OH), and the milk replacer + starter + mixed hay group (AO), in which the alfalfa hay and oat hay were administered in a 1:1 ratio. All calves in the three groups were fed the same milk replacer and an equivalent amount of dry matter.

Insights into flavor quality and metabolites profiles of fresh cheese with different probiotics by SPME-GC-MS and untargeted metabolomics.

In this study, fresh cheeses produced with four novel probiotics (Lactobacillus casei PB-LC39, Lactobacillus rhamnosus PB-LR76, Lactobacillus helveticus HH-LH17, and Lactobacillus plantarum HH-LP56) were named as LC, LR, LH, and LP, respectively. SPME-GC-MS and untargeted metabolomics were used to compare and analyze the flavor quality, metabolites and metabolic pathways of LC, LR, LH and LP, and the potential function of differential metabolites was emphasized. The results demonstrated that the incorporation of probiotics resulted in a significant increase in the number of volatile flavor compounds and varying flavor profiles within the cheese.

Rapid detection of corn moisture content based on improved ICEEMDAN algorithm combined with TCN-BiGRU model.

Rapid detection of corn moisture content(MC) during maturity is of great significance for field cultivation, mechanical harvesting, storage, and transportation management. However, cumbersome operation, time-consuming and labor-intensive operation were the bottleneck in the traditional drying process and dielectric parameter method. Thus, to overcome the above problems, a rapid detection method for corn MC based on improved complete ensemble empirical mode decomposition with adaptive noise (ICEEMDAN) combined with temporal convolutional network-bidirectional gated recurrent unit (TCN-BiGRU) model.

Depletion of regulatory T cells enhances the T cell response induced by the neoantigen vaccine with weak immunogenicity.

Background: The neoantigen vaccine has remarkable potential in treating advanced cancer due to its tumor specificity and ability to bypass central tolerance mechanisms. However, numerous neoantigens show poor immunogenicity, and the immune inhibitory factors of present in both tumors and tumor-draining lymph nodes impair the efficacy of cancer neoantigen vaccine. Eliminating immunosuppressive cells will improve the priming and expansion of anti-tumor immune cells induced by the vaccine.

The performance of a 100 m outdoor atmospheric simulation chamber in China.

A new outdoor atmospheric simulation chamber with a volume of ∼100 m, the largest one in Asia, was recently constructed in the Research Center for Eco-Environmental Sciences of the Chinese Academy of Sciences (Beijing, China). This Atmospheric Environment Simulation System (AESS-RCEES) is a hemispherical-shaped photoreactor constructed using sixteen stainless-steel brackets covered by double-layer Teflon film, offering two advantages over other outdoor chambers: 1) The AESS-RCEES effectively minimizes the "greenhouse effect" by continuously circulating cooling air through the double layer, resulting in mean temperature differences between the inside and outside of around 12 °C in winter and 9 °C in summer. 2) The chamber can quickly introduce ambient air through a special inlet system, completely replacing the air inside within 45 min.

Analysis of Nirmatrelvir Entry into Pulmonary Lining Fluid in Patients with COVID-19: A Unique Perspective to Explore and Understand the Target Plasma Concentration of 292 ng/mL in Antiviral Activity.

Background: Currently, the applicant has chosen a target plasma trough concentration for nirmatrelvir, which is adjusted to 292 ng/mL based on the drug's molecular weight (499.54 Daltons), its binding to human plasma proteins (69%), and the in vitro antiviral EC value (181 nM). However, the current exposure-effect relationships (ER) analysis of viral load in patients enrolled in the EPIC-HR study has not revealed clinically significant trends in the ER.

Diketopyrrolopyrrole-Activated Dynamic Condensation Approach to Narrow-Band Gap Vinylene-Linked Covalent Organic Frameworks.

Vinyl units intrinsically featuring less steric, nonpolarity, and unsaturated character, are well-known π-bridge used in the synthesis of high-performance semiconducting materials. Two-dimensional (2D) vinylene-linked covalent organic frameworks (COFs) represent a promising class of π-conjugated structures, however, the range of available monomers for the reversible formation of carbon-carbon double bonds remains limited. In this study, a new class of 2D vinylene-linked COFs were synthesized using dimethyldiketopyrrolopyrrole (DM-DPP) as the key monomer.