Multimodal single cell-resolved spatial proteomics reveal pancreatic tumor heterogeneity.

Despite the advances in antibody-guided cell typing and mass spectrometry-based proteomics, their integration is hindered by challenges for processing rare cells in the heterogeneous tissue context. Here, we introduce Spatial and Cell-type Proteomics (SCPro), which combines multiplexed imaging and flow cytometry with ion exchange-based protein aggregation capture technology to characterize spatial proteome heterogeneity with single-cell resolution. The SCPro is employed to explore the pancreatic tumor microenvironment and reveals the spatial alternations of over 5000 proteins by automatically dissecting up to 100 single cells guided by multi-color imaging of centimeter-scale formalin-fixed, paraffin-embedded tissue slide.

Double contrast-enhanced ultrasonography improves diagnostic accuracy of T staging compared with multi-detector computed tomography in gastric cancer patients.

Background: Gastric cancer (GC) is the most common malignant tumor and ranks third for cancer-related deaths among the worldwide. The disease poses a serious public health problem in China, ranking fifth for incidence and third for mortality. Knowledge of the invasive depth of the tumor is vital to treatment decisions.

Integrated approach for simultaneously measuring thermophysical parameters of semi-transparent materials.

To concurrently determine the thermophysical parameters of semi-transparent materials, a novel, to the best of our knowledge, integrated approach for concurrent measurement is proposed. In the measurement setup, a high-temperature radiation source and a beam reducer are employed to minimize the influence of background radiation. In order to differentiate between the transmitted and emitted radiation in the detection signal, the radiation signals from the radiation source are measured under four different conditions, enabling the calculation of transmissivity, emissivity, and reflectivity.

Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury.

As a primary target of severe acute respiratory syndrome coronavirus 2, lung exhibits heterogeneous histopathological changes following infection. However, comprehensive insight into their protein basis with spatial resolution remains deficient, which hinders further understanding of coronavirus disease 2019 (COVID-19)-related pulmonary injury. Here, we generate a region-resolved proteomic atlas of hallmark pathological pulmonary structures by integrating histological examination, laser microdissection, and ultrasensitive proteomics.

Delta Radiomics Based on Longitudinal Dual-modal Ultrasound Can Early Predict Response to Neoadjuvant Chemotherapy in Breast Cancer Patients.

Rationale And Objectives: To develop a monitoring model using radiomics analysis based on longitudinal B-mode ultrasound (BUS) and shear wave elastography (SWE) to early predict pathological response to neoadjuvant chemotherapy (NAC) in breast cancer patients.

Materials And Methods: In this prospective study, 112 breast cancer patients who received NAC between September 2016 and March 2022 were included. The BUS and SWE data of breast cancer were obtained prior to treatment as well as after two and four cycles of NAC.

Data processing for simultaneous inversion of emissivity and temperature using improved CABCSMA and target-to-best DE algorithms in multispectral radiation thermometry (MRT).

In this paper, what we believe to be, a new combined algorithm of artificial bee colony and slime mould algorithm (CABCSMA) and a differential evolution (DE) algorithm using target-to-best variation strategy are proposed to process the data based on Planck's radiation law and the mathematical model of reference temperature. The material model with 6 different emissivity trends is simulated. Simulation results show that the average relative error of CABCSMA algorithm is less than 0.

Optimizing MRT data processing via comparative analysis of SA and PSO algorithms: a simulation and numerical study.

The unknown emissivity still poses a significant challenge in the data processing of Multispectral radiation thermometry (MRT). In order to achieve global optimal solution with fast convergence speed and strong robustness, a systematic comparative analysis of particle swarm optimization (PSO) and simulated annealing (SA) algorithms in the application in MRT is presented in this paper. The simulations of six hypothetical emissivity models were compared, and the results indicate that the PSO algorithm is superior to the SA algorithm in accuracy, efficiency and stability.

Data processing method for simultaneous estimation of temperature and emissivity in multispectral thermometry.

The data processing in multispectral thermometry remains a huge challenge due to the unknown emissivity. In this article, a novel data processing model of multispectral thermometer is established by adding new constraints of emissivity on the basis of object function. The new two algorithms for model optimizing, Sequential Randomized Coordinate Shrinking (SRCS) and Multiple-Population Genetic (MPG), are introduced.

Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip.

Genotyping platforms, as critical supports for genomics, genetics, and molecular breeding, have been well implemented at national institutions/universities in developed countries and multinational seed companies that possess high-throughput, automatic, large-scale, and shared facilities. In this study, we integrated an improved genotyping by target sequencing (GBTS) system with capture-in-solution (liquid chip) technology to develop a multiple single-nucleotide polymorphism (mSNP) approach in which mSNPs can be captured from a single amplicon. From one 40K maize mSNP panel, we developed three types of markers (40K mSNPs, 251K SNPs, and 690K haplotypes), and generated multiple panels with various marker densities (1K-40K mSNPs) by sequencing at different depths.

Cathepsin B aggravates acute pancreatitis by activating the NLRP3 inflammasome and promoting the caspase-1-induced pyroptosis.

Objectives: Cathepsin B (CTSB), nod-like receptor family pyrin domain-containing 3 (NLRP3), and caspase-1 play an important role in the development of Acute Pancreatitis (AP). Besides, the relationship between the proteins remains poorly understood. In addition, whereas previous studies have found caspase-1 activation in AP, pyroptosis, a caspase-1 induced cell death mode, has never been proposed and proved in AP.

NNT-AS1 modulates prostate cancer cell proliferation, apoptosis and migration through miR-496/DDIT4 axis.

Background: Emerging studies have disclosed long non-coding RNAs (lncRNAs) as pivotal modulators in the progression of prostate cancer (PCa). Current research planned to figure out the involvement of lncRNA nicotinamide nucleotide transhydrogenase antisense RNA 1 (NNT-AS1) in PCa.

Methods: RNA expression was examined using RT-qPCR in PCa cells.

Copper-Mediated DNA-Compatible One-Pot Click Reactions of Alkynes with Aryl Borates and TMS-N.

We report a DNA-compatible copper-mediated efficient synthesis of 1,2,3-triazoles via a one-pot reaction of aryl borates with TMS-N followed by a click cycloaddition reaction. Employing the binuclear macrocyclic nanocatalyst Cu(II)-β-cyclodextrin, the reactions were performed under mild conditions with high conversions and wide functional group tolerance. We also demonstrate the reaction application toward a one-pot DNA-compatible intramolecular macrocyclization.

Palladium-mediated Suzuki-Miyaura Cross-Coupling Reaction of Potassium Boc-protected aminomethyltrifluoroborate with DNA-Conjugated aryl bromides for DNA-Encoded chemical library synthesis.

A mild reaction for DNA-compatible, palladium promoted Suzuki-Miyaura cross-coupling reaction of potassium Boc-protected aminomethyltrifluoroborate with DNA-conjugated aryl bromides has been developed efficiently. This novel DNA encoded chemistry reaction proceeded well with a wide range of functional group tolerance, including aryl bromides and heteroaryl bromides. Further, the utility our DNA conjugated aminomethylated arene products is demonstrated by reaction with various types of reagents (including amide formation with carboxylic acids, alkylation with aldehydes, and carbamoylation with amines) as would be desired for the production of a DNA encoded library.

Total Saponin from Root of Actinidia valvata Dunn Inhibits Hepatoma 22 Growth and Metastasis In Vivo by Suppression Angiogenesis.

The root of Actinidia valvata dunn has been widely used in the treatment of hepatocellular carcinoma (HCC), proved to be beneficial for a longer and better life in China. In present work, total saponin from root of Actinidia valvata Dunn (TSAVD) was extracted, and its effects on hepatoma H22-based mouse in vivo were observed. Primarily transplanted hypodermal hepatoma H22-based mice were used to observe TSAVD effect on tumor growth.

Asymmetric total synthesis and revision of the absolute configuration of 4-keto-clonostachydiol.

The first total synthesis of the 14-membered natural macrocyclic bislactone 4-keto-clonostachydiol, along with its enantiomer, has been accomplished in 13 steps with overall yields of 8.4% and 8.0%, respectively.

Total synthesis of (-)-bitungolide F.

An efficient total synthesis of (-)-bitungolide F (6) in 17 steps and 20.1% yield is described herein. Key steps involve a Myers asymmetric alkylation to introduce the C6 methyl with proper stereochemistry, a Claisen-like cyclization to construct the alpha,beta-unsaturated delta-lactone and a Julia-Kocienski olefination to assemble the conjugated diene moiety.

Application of a domino Friedel-Crafts acylation/alkylation reaction to the formal syntheses of (+/-)-taiwaniaquinol B and (+/-)-dichroanone.

An efficient acid-promoted domino Friedel-Crafts (FC) acylation/alkylation reaction has been developed for the construction of the core 6,5,6-ABC tricyclic skeleton of taiwaniaquinoids. The formal total syntheses of diterpenoids (+/-)-taiwaniaquinol B and (+/-)-dichroanone based on this strategy have been achieved.

6-Methyl-2,4-diphenyl-quinoline.

The mol-ecules of the title compound, C(22)H(17)N, are linked by weak inter-actions, among which the most prominent are C-H⋯π inter-actions. The dihedral angles between the phenyl rings and the quinoline ring system are 43.3 (3) and 21.