Fractionating the Flavonoids in Flos and via Solvent Extraction Coupled with Automated Solid-Phase Extraction.

Due to the structural diversity of flavonoids in functional plant foods and the inherent limitations of existing techniques, it is important to develop a simple and green (environmentally friendly) method of extracting flavonoids from plant foods. In this study, a method involving solvent extraction followed by automated solid-phase extraction was developed for extracting flavonoids from flos (JYH) and (SYH), both of which are widely used functional plant-based foods in Asian countries. For the optimisation of the solvent extraction method, solvent concentration (0.

Prokineticin 2 suppresses GABA-activated current in rat primary sensory neurons.

Prokineticin 2 (PK2) is a newly identified regulatory protein, which is involved in a wide range of physiological processes including pain perception in mammals. However, the precise role of PK2 in nociception is yet not fully understood. Here, we investigate the effects of PK2 on GABA(A) receptor function in rat trigeminal ganglion neurons using whole-cell patch clamp technique.