Development and collaborative validation of an event-specific quantitative real-time PCR method for detection of genetically modified CC-2 maize.

As one of the developed genetically modified (GM) maize varieties in China, CC-2 has demonstrated promising commercial prospects during demonstration planting. The establishment of detection methods is a technical prerequisite for effective supervision and regulation of CC-2 maize. In this study, we have developed an event-specific quantification method that targets the junction region between the exogenous gene and the 5' flanking genomic DNA (gDNA) of CC-2.

Counterion Blockade in a Heterogeneously Charged Single-File Water Channel.

The Possion-Nernst-Planck theories fail to describe the ionic transport in Angstrom channels, where conduction deviates from Ohm's law, which is attributed to the dehydration/self-energy barrier and dissociation of Bjerrum ion pairs in previous work. Here, we find that the cations can be strongly bound to the surface charge, which blocks the ionic transport in a single-file water channel, causing nonlinear current-voltage curves. The presence of free ions significantly increases the probability of bound ions being released, resulting in an ionic current.

Soft Memristor at a Microbubble Interface.

Memristors show promising features for neuromorphic computing. Here we report a soft memristor based on the liquid-vapor surface of a microbubble. The thickness of the liquid film was modulated by electrostatic and interfacial forces, enabling resistance switches.

Memristive Characteristics in an Asymmetrically Charged Nanochannel.

The emergent nanofluidic memristor provides a promising way of emulating neuromorphic functions in the brain. The conical-shaped nanopore showed promising features for a nanofluidic memristor, inspiring us to investigate the memory effects in asymmetrically charged nanochannels due to their high current rectification, which may result in good memory effects. Here, the memory effects of an asymmetrically charged nanofluidic channel were numerically simulated by Poisson-Nernst-Planck equations.

Nanofluidic memristor based on the elastic deformation of nanopores with nanoparticle adsorption.

The memristor is the building block of neuromorphic computing. We report a new type of nanofluidic memristor based on the principle of elastic strain on polymer nanopores. With nanoparticles absorbed at the wall of a single conical polymer nanopore, we find a pinched hysteresis of the current within a scanning frequency range of 0.

Ultralow Energy Consumption Angstrom-Fluidic Memristor.

The emergence of nanofluidic memristors has made a giant leap to mimic the neuromorphic functions of biological neurons. Here, we report neuromorphic signaling using Angstrom-scale funnel-shaped channels with poly-l-lysine (PLL) assembled at nano-openings. We found frequency-dependent current-voltage characteristics under sweeping voltage, which represents a diode in low frequencies, but it showed pinched current hysteresis as frequency increases.

Energy harvesting from water streaming at charged surface.

Water flowing at a charged surface may produce electricity, known as streaming current/potentials, which may be traced back to the 19th century. However, due to the low gained power and efficiencies, the energy conversion from streaming current was far from usable. The emergence of micro/nanofluidic technology and nanomaterials significantly increases the power (density) and energy conversion efficiency.

Lyophilization process optimization and molecular dynamics simulation of mRNA-LNPs for SARS-CoV-2 vaccine.

Some studies have shown that lyophilization significantly improves the stability of mRNA-LNPs and enables long-term storage at 2-8 °C. However, there is little research on the lyophilization process of mRNA-lipid nanoparticles (LNPs). Most previous studies have used empirical lyophilization with only a single lyoprotectant, resulting in low lyophilization efficiency, often requiring 40-100 h.

Synthesis of cell penetrating peptide sterol coupler and its liposome study on S-mRNA.

In order to overcome the current LNP-mRNA delivery system's weakness of poor stability and rapid degradation by nuclease, a novel chol-CGYKK molecule and then the new phospholipid liposome were designed and prepared. A solid phase approach synthesized CGYKK and connected it to cholesterol via a disulfide linker to form the desired chol-CGYKK. Four formulated samples with different proportions of excipients were prepared by freeze-drying cationic liposomes and packaged S-mRNA.

Xanthatin suppresses pancreatic cancer cell growth via the ROS/RBL1 signaling pathway: In vitro and in vivo insights.

Background: As a malignant digestive system tumor, pancreatic cancer has a high mortality rate. Xanthatin is a sesquiterpene lactone monomer compound purified from the traditional Chinese herb Xanthium strumarium L. It has been reported that Xanthatin exhibits inhibitory effects on various cancer cells in retinoblastoma, glioma, hepatoma, colon cancer, lung cancer, as well as breast cancer.

Surface-charge governed ionic blockade in angstrom-scale latent-track channels.

When channels are scaled down to the size of hydrated ions, Coulomb interactions are enhanced in confinement, resulting in new phenomena. Herein, we found blockade of ionic transport in latent-track angstrom-scale channels governed by surface charge, fundamentally different from Coulomb blockade or Wien effects. The channels are non-conductive at low voltage, blocked by cations bound at the surface in confinement; however, they change to conductive with increasing voltage due to the release of bound ions.

The CRISPR/Cas System: A Customizable Toolbox for Molecular Detection.

Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) are promising molecular diagnostic tools for rapidly and precisely elucidating the structure and function of genomes due to their high specificity, programmability, and multi-system compatibility in nucleic acid recognition. Multiple parameters limit the ability of a CRISPR/Cas system to detect DNA or RNA. Consequently, it must be used in conjunction with other nucleic acid amplification techniques or signal detection techniques, and the reaction components and reaction conditions should be modified and optimized to maximize the detection performance of the CRISPR/Cas system against various targets.

regulated by Huang-Qi-Ling-Hua-San is a key factor by which to improve type 2 diabetes.

There is a lot of evidence that oral hypoglycemic drugs work by affecting gut microbes, but the key strains responsible for this effect are not well known. Huang-Qi-Ling-Hua-San (HQLHS), composed of , , , and L., is a specially designed Chinese medicine formula to treat type 2 diabetes (T2D).

Expression and Immunogenicity of SARS-CoV-2 Virus-Like Particles based on Recombinant Truncated HEV-3 ORF2 Capsid Protein.

COVID-19 is an emerging disease that poses a severe threat to global public health. As such, there is an urgent demand for vaccines against SARS-CoV-2, the virus that causes COVID-19. Here, we describe a virus-like nanoparticle candidate vaccine against SARS-CoV-2 produced by an expression system.

Establishment and Validation of Reference Genes of L. for Digital PCR Detection of Genetically Modified Canola.

As an effective tool for genetically modified organism (GMO) quantification in complex matrices, digital PCR (dPCR) has been widely used for the quantification of genetically modified (GM) canola events; however, little is known about the quantification of GM canola events using endogenous reference gene () characteristics by dPCR. To calculate and quantify the content of GM canola using endogenous reference gene () characteristics, the suitability of several ERGs of canola, such as cruciferin A (), acetyl-CoA carboxylase (), phosphoenolpyruvate carboxylase (), cruciferin storage (), oleoyl hydrolase (), and high-mobility-group protein I/Y (), was investigated by droplet dPCR. and were more specific and stable in copy number in the genome of L.

Xanthatin inhibits non-small cell lung cancer proliferation by breaking the redox balance.

Lung cancer is the cancer with the highest mortality, and non-small cell lung cancer (NSCLC) accounts for more than 80%. Tumor cells often have high reactive oxygen species (ROS) and antioxidant capacity. Redox balance is very important for tumor.

Highly flexible elastomer microfluidic chip for single cell manipulation.

New materials and fabrication technologies have significantly boosted the development of lab-on-a-chip technologies and functionalities. In this work, we developed a highly flexible elastomer microfluidic chip with a microchannel with a minimum width of ∼5 m manufactured by imprinting onto an SU-8 template. We found that the deformation induced in the microstructures by manual stretching of the chip is higher than that for the chip itself, which we attribute to the stress concentration of microstructures.

Cationic liposomes co-deliver chemotherapeutics and siRNA for the treatment of breast cancer.

In order to improve the targeting efficiency and reduce anti-breast cancer therapeutic side effects, paclitaxel (PTX), crizotinib (CRI), and Bcl-xL siRNA were co-loaded in cationic liposomes (CTL), which exhibited a substantial enhanced permeability and retention effect (EPR effect) in breast cancer. CTL containing crizotinib and paclitaxel (CRI-PTX-CTL) had particle sizes of (138.63 ± 1.

Overexpression of an aquaporin protein from Aspergillus glaucus confers salt tolerance in transgenic soybean.

Salt stress is an important abiotic factor that causes severe losses in soybean yield and quality. Therefore, breeding salt-tolerant soybean germplasm resources via genetic engineering has gained importance. Aspergillus glaucus, a halophilic fungus that exhibits significant tolerance to salt, carries the gene AgGlpF.

Xanthatin inhibits human colon cancer cells progression via mTOR signaling mediated energy metabolism alteration.

Tumor cells exhibit higher glycolysis and rely on abnormal energy metabolism to produce ATP, which is essential for cell proliferation and migration. Abnormal energy metabolism inhibition is considered a promising tumor treatment strategy. Xanthatin is an active sesquiterpene lactone isolated from Xanthium strumarium L.

Temperature Induced Dimensional Tuning and Anomalous Deformation of Micro/Nanopores.

Artificial nanopores have become a common toolbox in nanotechnologies, with dimension and geometry as predominant factors. Most fabrication technologies determine the pore size beforehand, but few exist that enable size-tuning post-manufacturing. In this work, we reported a type of ion track etched micro/nanopores on uniaxially drawn PET foils that enable irreversible thermal shrinkage, thus tuning the pore dimensions by increasing ambient temperatures.

Organ-on-a-chip: the next generation platform for risk assessment of radiobiology.

Organ-on-a-chip devices have been widely used in biomedical science and technology, for example for experimental regenerative medicine and precision healthcare. The main advantage of organ-on-a-chip technology is the facility to build a specific human model that has functional responses on the level of organs or tissues, thereby avoiding the use of animal models, as well as greatly improving new drug discovery processes for personal healthcare. An emerging application domain for organs-on-chips is the study of internal irradiation for humans, which faces the challenges of the lack of a clear model for risk estimation of internal irradiation.

Liquid-Solid Slip on Charged Walls: The Dramatic Impact of Charge Distribution.

Nanofluidic systems show great promise for applications in energy conversion, where their performance can be enhanced by nanoscale liquid-solid slip. However, efficiency is also controlled by surface charge, which is known to reduce slip. Combining molecular dynamics simulations and analytical developments, we show the dramatic impact of surface charge distribution on the slip-charge coupling.

Epicardial transplantation of atrial appendage micrograft patch salvages myocardium after infarction.

Background: Ischemic heart disease remains the leading cause of mortality and morbidity worldwide despite improved possibilities in medical care. Alongside interventional therapies, such as coronary artery bypass grafting, adjuvant tissue-engineered and cell-based treatments can provide regenerative improvement. Unfortunately, most of these advanced approaches require multiple lengthy and costly preparation stages without delivering significant clinical benefits.

Origin of nonequilibrium 1/f noise in solid-state nanopores.

Nanopore devices are applied in many fields such as molecular sensing and DNA sequencing, and the detection precision is primarily determined by 1/f noise. The mechanism of 1/f noise in nanopores is still not clearly understood, especially the nonequilibrium 1/f noise in rectifying nanopores. Hereby, we propose that 1/f noise in solid-state nanopores originates from the electrolyte ion trapping-detrapping process occurring on the inner surface of the nanopores, which can nonlinearly affect the ion number inside the rectifying nanopores due to the specific ion enrichment/depletion effect.