New strategy for high-level expression and purification of biologically active monomeric TGF-β1/C77S in Escherichia coli.

Mature transforming growth factor beta1 (TGF-β1) is a homodimeric protein with a single disulfide bridge between Cys77 on the respective monomers. The synthetic DNA sequence encoding the mature human TGF-β1/C77S (further termed TGF-β1m) was cloned into plasmid pET-32a downstream to the gene of fusion partner thioredoxin (Trx) immediately after the DNA sequence encoding enteropeptidase recognition site. High-level expression (~1.

Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase.

Enteropeptidase (EC 3.4.21.