Quercetin can improve anesthesia induced neuroinflammation and cognitive dysfunction by regulating miR-138-5p/ LCN2.

Background: Anesthesia can lead to functional cognitive impairment, which can seriously affect postoperative recovery. To investigate the effect and mechanism of quercetin (Que) in anesthetized rats, the study provided a new therapeutic idea for the prevention of cognitive dysfunction caused by anesthesia.

Methods: Cognitively impaired rats were constructed using Isoflurane (ISO) anesthesia and treated with Que.

Elucidating Manganese Single-Atom Doping: Strategies for Fluorescence Enhancement in Water-Soluble Red-Emitting Carbon Dots and Applications for FL/MR Dual Mode Imaging.

The absence of the enhancement of fluorescence in carbon dots (CDs) through doping with transition metal atoms (TMAs) hinders the advancement of multi-modal bio-imaging CDs with high photoluminescence quantum yield (PLQY). Herein, Mn-atomically-doped R-CDs (R-Mn-CDs) with a high PLQY of 41.3% in water is presented, enabling efficient in vivo dual-mode fluorescence/magnetic resonance (MR) imaging.

BiWO@CuO-GO bio-heterojunction spray for accelerating chronic diabetic wound repairment with bilaterally enhanced sono-catalysis and glycolytic inhibition antisepsis.

Chronic diabetic wound poses a pressing global healthcare challenge, necessitating an approach to address issues such as pathogenic bacteria elimination, blood sugar regulation, and angiogenesis stimulation. Herein, we engineered a BiWO@CuO-GOx bio-heterojunction (BWCG bio-HJ) with exceptional cascade catalytic performance and impressive sonosensitivity to remodel the wound microenvironment and expedite the diabetic wound healing. Specifically, the Z-scheme junctions of BiWO@CuO significantly augmented carrier separation dynamics, leading to the highly efficient generation of reactive oxygen species (ROS) upon US irradiations.

Targeted thermosensitive liposomes loaded with gold nanoparticles and temozolomide hexadecanoate for the synergistic photothermal-chemotherapy treatment of glioblastoma.

Glioblastoma (GBM) is a highly aggressive malignant brain tumour which presents a significant challenge due to the limited effectiveness of current surgical and chemotherapeutic approaches. In this study, we have developed TMZ16e and gold nanoparticles coencapsulated thermosensitive liposomes modified with anti-EphA3 (anti-EphA3-TMZ16e-GNPs-TSL) delivered via the intranasal route to achieve photothermal chemotherapy (PCT) for improving the therapeutic effects of GBM. The prepared anti-EphA3-TMZ16e-GNPs-TSL were spherical with a particle size of 173.

Study on the Chemical Characterization and Hypolipidemic Function of Based on Its Flavonoid Components.

has great value and development prospects in hypolipidemic applications. In this study, we comprehensively screened out multi-index components relevant to the quality of based on the hypolipidemic function of the flavonoid fraction of (FFN) combined with chemical characterizations. Firstly, in vitro antioxidant and cell experiments evaluated the hypolipidemic function of the FFN.

Molecular identification and subtyping of Cryptosporidium spp. in laboratory mice and rats.

Cryptosporidium species can infect humans and more than 260 animal species, including 54 rodent species. However, data on the occurrence and genetic characterizations of Cryptosporidium spp. in laboratory rodents are limited.

Identification of heterozygous mutations of ABCC8 gene responsible for maturity-onset diabetes of the young with exome sequencing.

Background: Although the MODY12 subtype, caused by ABCC8 mutations, is rare, it is highly sensitive to sulfonylureas. The identification of ABCC8 mutations in patients clinically diagnosed with MODY has the ability to contribute to the precise management of diabetes.

Methods: Genetic analysis of two families with MODY were conducted using whole-exome sequencing (WES) and Sanger sequencing.

Self-Adaptive Layering Structure of Nanoconfined Fe-Ni Liquids: Origin of the Liquid-Liquid Phase Transition.

Metallic liquids under confinement exhibit different properties compared to those of their corresponding bulk phases, such as miscibility, diffusion, and phase transitions. Unfortunately, the challenges in experimentally characterizing Fe-Ni liquids at the nanoscale and the high cost of first-principles simulations hindered the atom-level understanding that is necessary for controlling Fe-Ni liquids. Here, we report a comprehensive molecular dynamics study of the liquid Fe-Ni alloy confined within nanoslits.

A Case of Pulmonary Infection Due to in a Non-Immunocompromised Patient with Cerebral Palsy.

Background: () is a dimorphic opportunistic fungus that is a rare yeast and rarely reported in Asia. Owing to the absence of established clinical breakpoints, the treatment of this fungus poses challenges.

Case Presentation: We report a rare case of a young, non-immunocompromised man with cerebral palsy, spinal deformity, and pulmonary infection.

Wastewater-based intestinal protozoa monitoring in Shanghai, China.

Intestinal protozoa spp., , and have been implicated in serious waterborne outbreaks worldwide. Wastewater-based epidemiology (WBE) is a promising approach for evaluating the disease prevalence in a catchment population in that it monitors the contamination level of the intestinal pathogens in wastewater.

Identification and quantitative analysis of genotoxic impurities in rifampicin: Development and validation of a targeted LC-MS/MS method for 1-amino-4-methylpiperazine.

Rifampicin, essential for long-term tuberculosis treatment, requires rigorous control of non-therapeutic impurities due to their potential adverse, including mutagenic effects. Reports on control strategies for genotoxic impurities in rifampicin have been limited. This study introduced an analytical method to identify potential genotoxic impurities from the synthesis of raw materials.

Brown rats () as potential reservoirs of in Heilongjiang Province, China: high prevalence, genetic heterogeneity, and potential risk for zoonotic transmission.

Introduction: , an obligatory intracellular fungus, is prevalent among animals and humans. Due to their close interaction with humans and their extensive regional distribution, brown rats () are important pathogen reservoirs. To assess the zoonotic transmission potential of , a molecular investigation was conducted on 817 from four cities in Heilongjiang Province, China.

Combining salivary α-synuclein seeding activity and miRNA-29a to distinguish Parkinson's disease and multiple system atrophy.

Introduction: The differential diagnosis of early Parkinson's disease (PD) by a single biomarker is still challenging due to its symptomatic overlap with other neurological diseases. Increasing evidences support the use of saliva biomarkers of neurodegeneration, including microRNAs and α-synuclein (α-syn) seeding activity, to diagnose patients with idiopathic PD and multiple system atrophy (MSA). Our previous study confirmed the salivary microRNA-29a-3p (miRNA-29a-3p) and α-syn seeding activity could differentiate PD and MSA from healthy control subjects (HCs) and patients with essential tremor (ET).

Dopaminergic Neurons in Zona Incerta Drives Appetitive Self-Grooming.

Dopaminergic (DA) neurons are known to play a key role in controlling behaviors. While DA neurons in other brain regions are extensively characterized, those in zona incerta (ZI or A13) receive much less attention and their function remains to be defined. Here it is shown that optogenetic stimulation of these neurons elicited intensive self-grooming behaviors and promoted place preference, which can be enhanced by training but cannot be converted into contextual memory.

Photo-Activated Oxidative Stress Amplifier: A Strategy for Targeting Glutathione Metabolism and Enhancing ROS-Mediated Therapy in Triple-Negative Breast Cancer Treatment.

Amplifying oxidative stress within tumor cells can effectively inhibit the growth and metastasis of triple-negative breast cancer (TNBC). Therefore, the development of innovative nanomedicines that can effectively disrupt the redox balance represents a promising yet challenging therapeutic strategy for TNBC. In this study, an oxidative stress amplifier, denoted as PBCH, comprising PdAg mesoporous nanozyme and a CaP mineralized layer, loaded with GSH inhibitor L-buthionine sulfoximine (BSO), and further surface-modified with hyaluronic acid that can target CD44, is introduced.

Metal ion interference therapy: metal-based nanomaterial-mediated mechanisms and strategies to boost intracellular "ion overload" for cancer treatment.

Metal ion interference therapy (MIIT) has emerged as a promising approach in the field of nanomedicine for combatting cancer. With advancements in nanotechnology and tumor targeting-related strategies, sophisticated nanoplatforms have emerged to facilitate efficient MIIT in xenografted mouse models. However, the diverse range of metal ions and the intricacies of cellular metabolism have presented challenges in fully understanding this therapeutic approach, thereby impeding its progress.

Cross-species transmission of Cryptosporidium in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health.

Wild rodents serve as reservoirs for Cryptosporidium and are overpopulated globally. However, genetic data regarding Cryptosporidium in these animals from China are limited. Here, we have determined the prevalence and genetic characteristics of Cryptosporidium among 370 wild rodents captured from three distinct locations in the southern region of Zhejiang Province, China.

Chemical structure and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix.

A new polysaccharide, named SP40015A01, was obtained from Saposhnikoviae Radix by water extraction, isolation and purification. SP40015A01 (9.7 × 10 Da) is composed of Rhamnose (Rha), Galacturonic acid (GalA), Galactose (Gal), and Arabinose (Ara) with the proportion of 1.

Missense mutation of ISL1 (E283D) is associated with the development of type 2 diabetes.

Aims/hypothesis: Mutations in Isl1, encoding the insulin enhancer-binding protein islet-1 (ISL1), may contribute to attenuated insulin secretion in type 2 diabetes mellitus. We made an Isl1 mouse model to investigate the disease-causing mechanism of diabetes mellitus.

Methods: The ISL1 mutation (c.