Solid-state nanopore is a promising single molecular detection technique, but is largely limited by relatively low resolution to small-size targets and laborious design of signaling probes. Here we establish a universal, CRISPR/Cas-Assisted Nanopore Operational Nexus (CANON), which can accurately transduce different targeting sources/species into different DNA structural probes via a "Signal-ON" mode. Target recognition activates the cleavage activity of a Cas12a/crRNA system and then completely digest the blocker of an initiator.
View Article and Find Full Text PDFFunctional redundancy is considered a pivotal mechanism for maintaining the adaptability of species by preventing the loss of key functions in response to dehydration. However, we still lack a comprehensive understanding of the redundancy of leaf hydraulic systems along aridity gradients. Here, photosynthesis (A), stomatal conductance (g) and leaf hydraulic conductance (K) during dehydration were measured in 20 woody species from a range of aridity index (AI) conditions and growing in a common garden to quantify stomatal redundancy (SR), the extent of stomatal opening beyond the optimum required for maximum photosynthesis (A), leaf hydraulic redundancy (HR), and the extent of leaf hydraulic conductance (K) beyond the optimum required for maximum g (g).
View Article and Find Full Text PDFIntroduction: Antimicrobial peptides (AMPs) present a promising avenue to combat the growing threat of antibiotic resistance. The ruminant gastrointestinal microbiome serves as a unique ecosystem that offers untapped potential for AMP discovery.
Objectives: The aims of this study are to develop an effective methodology for the identification of novel AMPs from ruminant gastrointestinal microbiomes, followed by evaluating their antimicrobial efficacy and elucidating the mechanisms underlying their activity.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
January 2025
The variability of the apnea-hypopnea index(AHI) measured in the first and second halves of the night is significant in patients with obstructive sleep apnea hypopnea syndrome(OSAHS). This variation may be related to fluid redistribution caused by the supine position during sleep. Eighty-nine adult subjects were enrolled.
View Article and Find Full Text PDFStudy Objectives: Obstructive sleep apnea (OSA) is a common disorder in the pediatric population, primarily diagnosed through polysomnography (PSG). However, PSG can be expensive and is often limited in availability. This study aimed to develop a cost-effective diagnostic strategy by integrating a screening model with PSG.
View Article and Find Full Text PDFOrganic photodiodes (OPDs) that utilize wavelength-selective absorbing molecules offer a direct approach to capturing specific wavelengths of light in multispectral sensors/imaging systems without filters. However, they exhibit broad response bandwidths, low external quantum efficiency (EQE), and often require compromises in two-component photoactive materials. Herein, the first utility of boron-nitrogen (BN) single-component photoabsorbers, leveraging a multi-resonance effect are introduced to attain OPDs with both record-high EQE of 33.
View Article and Find Full Text PDFThe U.S. Food and Drug Administration (FDA) recently approved lecanemab and donanemab for the treatment of early symptomatic Alzheimer's disease (AD) after their phase III trials reached endpoints.
View Article and Find Full Text PDFStaggered gap p-n heterojunction ZnO nanorods/AgO nanoparticles, a paradigm of photocatalysts, were developed via engineering the hydrothermal and coprecipitation method. Under simulated sunlight, the photocatalytic characteristics of ZnO/AgO(Zn/A) heterojunctions with varying mole ratios (from 8:1 to 8:4, named Zn/A-1-Zn/A-4) were systematically evaluated through the degradation of methylene blue (MB). The influence of key experimental variables, including photocatalyst concentration, MB concentration, and solution pH, on the photocatalyst performance was further analyzed.
View Article and Find Full Text PDFThe rapid evolution of multifunctional wearable smart devices has significantly expanded their applications in human-computer interaction and motion health monitoring. Central to these devices are flexible sensors, which require high stretchability, durability, self-adhesion, and sensitivity. Biomacromolecules have attracted attention in sensor design for their biocompatibility, biodegradability, and unique mechanical properties.
View Article and Find Full Text PDFIntroduction: Artificial intelligence is already widely utilized in gastroenterology. This study aims to comprehensively evaluate the research hotspots and development trends within the field of AI in gastroenterology by employing bibliometric techniques to scrutinize geographical distribution, authorship, affiliated institutions, keyword usage, references, and other pertinent data contained within relevant publications.
Methods: This investigation compiled all pertinent publications related to artificial intelligence in the context of gastrointestinal polyps and digestive endoscopy from 2003 to 2023 within the Web of Science Core Collection database.
Sustained or chronic inflammation in the placenta can result in placental insufficiency, leading to adverse reproductive outcomes such as pregnancy loss. Branched-chain amino acid transaminase 1 (BCAT1) expresses in the placenta and is involved in the pathological inflammatory response, but its role in recurrent miscarriage (RM) has not been fully investigated. In the present study, we delved into the effects of BCAT1 on trophoblast inflammation induced by lipopolysaccharide (LPS) and a mouse model of pregnancy loss induced by LPS.
View Article and Find Full Text PDFAppl Spectrosc
September 2024
Struct Heart
September 2024
Background: The implications of pulmonary vein (PV) flow patterns in patients with heart failure (HF) and mitral regurgitation (MR) are uncertain. We examined PV flow patterns in the Cardiovascular Outcomes Assessment of the MitraClip Percutaneous Therapy for Heart Failure Patients With Functional Mitral Regurgitation (COAPT) trial (NCT01626079), in which patients with HF and moderate-to-severe or severe functional MR were randomized to transcatheter edge-to-edge repair (TEER) with the MitraClip device plus guideline-directed medical therapy (GDMT) vs. GDMT alone.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
Anchoring Pt onto multi-heteroatom doped carbon materials has been recognized as an effective approach to improve the performance of electrocatalytic methanol oxidation. However, distinct contributions and specific behavior mechanisms of different heteroatoms, notably N and P, the specific behavior mechanisms in synergistically promoting Pt NPs remain elusive. In this work, we construct 1D N and P co-doped carbon nanotube (N, P-CNTs) supports with abundant defect anchors for Pt.
View Article and Find Full Text PDFJ Soc Cardiovasc Angiogr Interv
May 2024
Background: Transcatheter edge-to-edge repair (TEER) improved outcomes in patients with heart failure (HF) and severe secondary mitral regurgitation (SMR) compared with guideline-directed medical therapy (GDMT) alone regardless of the severity of baseline left ventricular ejection fraction (LVEF). The study aimed to evaluate the effect of early changes in LVEF after TEER and GDMT alone in patients with HF and severe SMR.
Methods: Within the COAPT trial, we evaluated outcomes according to changes in LVEF from baseline to 30 days.
Wild-type Lactococcus lactis strain LAC460 secretes prophage-encoded bacteriocin-like lysin LysL, which kills some Lactococcus strains, but has no lytic effect on the producer. LysL carries two N-terminal enzymatic active domains (EAD), and an unknown C-terminus without homology to known domains. This study aimed to determine whether the C-terminus of LysL carries a cell wall binding domain (CBD) for target specificity of LysL.
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