One-pot hydrothermal synthesis of polyethyleneimine-coated magnetic nanoparticles for high-efficient DNA extraction of pathogenic bacteria.

For separation of deoxyribonucleic acid (DNA), positively charged amino-modified magnetic nanoparticles (MN) can effectively adsorb negatively charged DNA through electrostatic interaction. However, the reported preparation of amino-modified MN is usually tedious and time-consuming. Therefore, a simple synthesis method of amino-modified MN is necessary for DNA extraction.

Treatment of limb skin defect with self-made skin distractor.

Objective: To investigate the effect of self-made skin distractor in the treatment of limb skin defects.

Methods: From September 2018 to January 2020, 15 cases of limb skin defects (16 wounds) were treated with self-designed skin distractor, including 13 males and 2 females, with an average age of 42 years (range, 9-73 years). The smallest area of skin defect was 6 cm×3 cm, the largest was 32 cm ×7 cm, with an average of 72.

ROS Responsive Cerium Oxide Biomimetic Nanoparticles Alleviates Calcium Oxalate Crystals Induced Kidney Injury via Suppressing Oxidative Stress and M1 Macrophage Polarization.

Emerging studies have demonstrated that M1 macrophage polarization and oxidative stress play important roles in calcium oxalate (CaOx) induced kidney injury, which leads to increased crystals deposition. ROS scavenging nanozymes and kidney-targeted nanoparticles for antioxidant drugs delivery have emerged as an arisen methodology for kidney injury therapy. However, cell membrane biomimetic-modified nanozymes as anti-inflammatory drug delivery systems for the treatment of kidney injury is rarely reported.

Brain networks in newborns and infants with and without sensorineural hearing loss: A functional near-infrared spectroscopy study.

Background: Understanding the impact of early sensory deficits on brain development is essential for understanding developmental processes and developing potential interventions. While previous studies have looked into the impact of prenatal experiences on language development, there is a lack of research on how these experiences affect early language and brain function development in individuals with sensorineural hearing loss (SNHL).

Aim: To investigate SNHL effects on early brain development and connectivity in 4-month-olds healthy newborns and controls.

The impact of anterior knee displacement on knee joint load during the forward bow step in Tai Chi.

Background: While the forward bow step is a crucial component of Tai Chi (TC) practice, little research has been conducted on its impact on knee joint load and muscle coordination. This study aims to investigate the effects of three different knee forward positions during the TC forward bow step on knee joint loading.

Methods: Twenty TC practitioners were recruited, and motion capture systems, force platforms, and surface electromyography were utilized to synchronously collect biomechanical parameters of three types of forward bow steps: knee joint not exceeding the tip of the foot (NETT), knee joint forward movement level with the tip of the foot (LTT), and knee joint forward movement exceeding the tip of the foot (ETT).

Purification, structural characterization and immunomodulatory activity of a polysaccharide isolated from Scutellaria baicalensis stem-leaf.

In our research, a novel polysaccharide (named SSP-3a) with uniform molecular weight was extracted from Scutellaria baicalensis stem-leaf. The structural analysis revealed that SSP-3a was an acidic polysaccharide with a heavy average molecular weight of 1.83 × 10 Da.

Single-cell RNA sequencing reveals a high-resolution cell atlas of petals in at different flowering development stages.

(mei), a traditional ornamental plant in China, is renowned for its fragrant flowers, primarily emitted by its petals. However, the cell types of mei petals and where floral volatile synthesis occurs are rarely reported. The study used single-cell RNA sequencing to characterize the gene expression landscape in petals of 'Fenhong Zhusha' at budding stage (BS) and full-blooming stage (FS).

Disrupting the olfactory response in Monochamus saltuarius: Two potential target genes for controlling the vector insect of pine wilt disease.

Worldwide, pine forests have been threatened by a devastating pine wood disease caused by Bursaphelenchus xylophilus, with Monochamus saltuarius being a newly recorded vector of the disease in Northeast China. The olfactory system plays important roles in both feeding and oviposition during the adult stage of M. saltuarius, and olfactory gene function research is essential for gaining an understanding of the olfactory mechanisms of this pest.

Enhanced sludge granulation and stable performance of an anammox expanded granular sludge bed (EGSB) reactor through the utilization of hydroxyapatite (HAP) particles.

The reuse of hydroxyapatite particles (HAPs) as a granulation activator for anammox sludge was explored to address the remaining issues of time-consuming and unstable granular structure in anammox granulation. During the granulation, nitrogen removal capacity from 2.8 to 13.

Role of comammox bacteria in granular bioreactor for nitrogen removal via partial nitritation/anammox.

In this study, two bioprocess models were first constructed with the newly-discovered comammox process described as one-step and two-step nitrification and evaluated against relevant experimental data. The validated models were then applied to reveal the potential effect of comammox bacteria on the granular bioreactor particularly suitable for undertaking partial nitritation/anammox (PN/A) under different operating conditions of bulk dissolved oxygen (DO) and influent NH. The results showed although comammox bacteria-based PN/A could achieve > 80.

Screening and Identification of Soil Selenium-Enriched Strains and Application in .

Selenium (Se) is an essential trace element for human physiological metabolism. The application of organic Se as a source to cultivate Se-rich plants for micronutrient supplementation has been receiving increasing attention. In our study, a bacterial strain named H1 was isolated from the soil in Heilongjiang Province, China, and under optimal culture conditions, the unit Se content could reach 3000 μg·g and its 16S ribosomal DNA sequence seemed to be a new molecular record of an species.

Nanoparticles targeting the adenosine pathway for cancer immunotherapy.

Cancer immunotherapy, as an emerging approach to cancer treatment, has tremendous potential for application. Compared to traditional methods such as surgery, chemotherapy, and radiation therapy, it has the ability to restore the patient's immune system, leading to long-term immune memory with less damage to normal tissues. However, immunotherapy has its limitations, including limited therapeutic efficacy, restricted patient populations, and inconsistent treatment responses.

Metabolite Profiling and Biological Activity Assessment of Anthers and Pollen Using UPLC-QTOF-MS.

is an important economic oil and medicinal crop. Its anthers are often used to make tea in China with beneficial effects on human health. However, the metabolite profiles, as well as potential biological activities of anthers and the pollen within anthers have not been systematically analyzed, which hinders the improvement of utilization.

Interspecies electron transfer and microbial interactions in a novel Fe(II)-mediated anammox coupled mixotrophic denitrification system.

This study effectively coupled anammox and mixotrophic denitrification at a high nitrogen load rate of 6.84 g N/L/d with 40 mg/L Fe(II). Fe(II) enhanced the activity of nitrate reductase, nitrite reductase, and hydrazine dehydrogenase enzymes, facilitating accelerated ATP synthesis.

A Set of Artificial Pollination Technical Measures: Improved Seed Yields and Active Ingredients of Seeds in Oil Tree Peonies.

The tree peony, a novel woody oil crop extensively cultivated in China, necessitates further investigation into artificial pollination technology to enhance seed yield. In this study, we conducted artificial pollination experiments with 6-year-old 'Feng Dan' seedings for suitable pollen sources, pollen concentration, pollination timing, and pollination frequency. By evaluating seed yields, active ingredients, and oil quality, we derived the following significant conclusions.

Which horticultural activities are more effective for children's recovery from stress and mental fatigue? A quasi-experimental study.

Introduction: Studies have established the benefits of horticultural therapy and activities for human health and well-being. Nonetheless, limited research has been conducted on the potential restorative advantages and distinctions between different types of horticultural activities in terms of stress reduction.

Methods: This study employed a quantitative research method to investigate the stress recovery benefits of five horticultural activities (flower arrangement, sowing and transplanting seeds, crafting, pressed flower card making, and decorative bottle painting with dried flowers) and one reference activity (short composition writing) for children.

The Development of the Stress-Free Polishing System Based on the Positioning Error Analysis for the Deterministic Polishing of Jet Electrochemical Machining.

Deterministic polishing based on jet electrochemical machining (Jet-ECM) is a stress-free machining method for low-rigidity and ultra-precision workpieces. The nozzle is equivalent to a special tool in deterministic polishing, and the workpiece material is removed using the mechanism of electrochemical dissolution at the position where the nozzle passes. By precisely regulating the nozzle's movement speed and dwell time, the quantity of material removed from the workpiece at a designated position can be finely adjusted.

Refracture-related bone transport of tibia: technical notes and preliminary clinical results in nineteen cases.

Purpose: Refracture is one of the main complications of bone transport, which brings additional physical and mental burden to surgeries and patients. We aimed to raise a new classification system of refracture-related bone transport based on the Simpson classification and to present our experience on treatment.

Methods: This retrospective analysis included 19 patients with refracture-related bone transport (average age of 37.

The integrate profiling of single-cell and spatial transcriptome RNA-seq reveals tumor heterogeneity, therapeutic targets, and prognostic subtypes in ccRCC.

Clear-cell renal cell carcinoma (ccRCC) is the most common type of RCC; however, the intratumoral heterogeneity in ccRCC remains unclear. We first identified markers and biological features of each cell cluster using bioinformatics analysis based on single-cell and spatial transcriptome RNA-sequencing data. We found that gene copy number loss on chromosome 3p and amplification on chromosome 5q were common features in ccRCC cells.

Transcriptome and volatile compounds analyses of floral development provide insight into floral scent formation in 'Wu Hua Long Yu'.

Herbaceous peony () is a well-known ornamental plant in China, celebrated for its beautiful flowers that can emit fragrances. However, exact molecular mechanisms governing synthesis of floral volatiles within herbaceous peony remain unclear. To address this gap in knowledge, our study focused on analyzing the transcriptome and the levels of floral volatile compounds in 'Wu Hua Long Yu' at different stages of flower development.

Recent Advances in Nanomaterials for the Treatment of Acute Kidney Injury.

Acute kidney injury (AKI) is a serious clinical syndrome with high morbidity, elevated mortality, and poor prognosis, commonly considered a "sword of Damocles" for hospitalized patients, especially those in intensive care units. Oxidative stress, inflammation, and apoptosis, caused by the excessive production of reactive oxygen species (ROS), play a key role in AKI progression. Hence, the investigation of effective and safe antioxidants and inflammatory regulators to scavenge overexpressed ROS and regulate excessive inflammation has become a promising therapeutic option.

An optimized approach for solar concentrating parabolic dish based on particle swarm optimization-genetic algorithm.

Parabolic dish concentrators have demonstrated the highest thermal and optical efficiencies among the available concentrator options. This paper proposes a novel design approach for fabricating large parabolic dish concentrators by employing compliant petals optimized through Particle Swarm Optimization-Genetic Algorithm (PSO-GA). The design concept involves using cables to pull the outer corners of the petals towards the center, resulting in the creation of finely formed dish mirrors.