[Chemical constituents from stems and leaves of Mycetia hainanensis and their anti-rheumatoid arthritis activities].

The chemical constituents from the stems and leaves of Mycetia hainanensis were isolated by silica gel, ODS gel, and Sephadex LH-20 gel column chromatography and preparative HPLC. The chemical structures of all the isolated compounds were identified on the basis of their physicochemical properties, spectroscopic data, as well as the comparison of their physicochemical and spectroscopic data with the reported data in literature. Twelve compounds were isolated from the 85% ethanol extract of the stems and leaves of M.

Structurally diverse monoterpenoid indole alkaloids from as well as their potential anti-inflammatory and anti-HIV effects.

A phytochemical investigation on the stems and leaves of caused the isolation and characterisation of a new monoterpenoid indole alkaloid, damnahainanline (), as well as seven known analogues (-). The molecular structure of was determined in the light of comprehensive spectroscopic data analyses and the known analogues - were determined comparing their experimental spectroscopic data with the reported spectroscopic data in the literature. The anti-inflammatory effects as well as anti-HIV activities of all isolates - were assessed.

Unraveling the influences of hemodynamic lag and intrinsic cerebrovascular reactivity on functional metrics in ischemic stroke.

Resting-state functional magnetic resonance imaging (rs-fMRI) is a prominent tool for investigating functional deficits in stroke patients. However, the extent to which the hemodynamic lags (LAG) and the intrinsic cerebrovascular reactivity (iCVR) may affect the rs-fMRI metrics in different scales needs to be clarified for ischemic stroke. In this study, 73 ischemic stroke patients and 74 healthy controls (HC) were recruited to investigate how the correction of the LAG and/or iCVR would influence resting-state functional magnetic resonance imaging (rs-fMRI) metrics of three different spatial scales (local-scale, meso-scale and global-scale) in ischemic stroke.

2-Arylbenzofurans from the stems and leaves of and their potential antiproliferative activities.

Phytochemical study on the stems and leaves of led to the isolation of a new 2-arylbenzofuran, artocartone (), as well as seven known 2-arylbenzofurans (). The chemical structure of was established by means of comprehensive spectroscopic analyses and the known compounds were determined by comparing their MS and NMR data with those reported data in literature. The antiproliferative activities of all isolates against five human cancer cell lines: HL-60, SMMC-7721, A-375, MCF-7 and SW480 were evaluated.

Polyprenylated xanthones with potential anti-inflammatory and anti-HIV effects from the stems and leaves of (Lour.) Blume.

A phytochemical study on the stems and leaves of (Lour.) Blume resulted in the isolation and characterisation of a new polyprenylated xanthone, cratocochinone (), as well as seven known analogues, fuscaxanthone K (), pruniflorone Q (), 1,3,5,8-tetrahy-droxy- 2-(3-methybut-2-enyl)-4-(3,7-dimethylocta-2,6-dienyl) xanthone (), cochinensoxanthone (), cratoxylum-xanthone B (), cochinchinone I () and cochinchinone K (). The chemical structure of was determined by comprehensive spectral analyses.

[Chemical constituents from stems and leaves of Artocarpus tonkinensis and their inhibitory effects on proliferation of synovioblasts in vitro].

The chemical constituents from the stems and leaves of Artocarpus tonkinensis in Artocarpus of Moraceae were systematically studied by means of silica gel, octadecylsilyl(ODS), and Sephadex LH-20 gel column chromatographies, as well as preparative high-performance liquid chromatography(Pre-HPLC) and a variety of chromatographic separation techniques. The spectral data and physicochemical properties of the compounds were obtained from separation and compared with those of the compounds reported in the literature. As a result, 11 compounds isolated from the 90% ethanol extract of the stems and leaves of A.

Abnormal intrinsic functional hubs and connectivity in patients with post-stroke depression.

Objective: The present study aimed to investigate the specific alterations of brain networks in patients with post-stroke depression (PSD), and further assist in elucidating the brain mechanisms underlying the PSD which would provide supporting evidence for early diagnosis and interventions for the disease.

Methods: Resting-state functional magnetic resonace imaging data were acquired from 82 nondepressed stroke patients (Stroke), 39 PSD patients, and 74 healthy controls (HC). Voxel-wise degree centrality (DC) conjoined with seed-based functional connectivity (FC) analyses were performed to investigate the PSD-related connectivity alterations.

Development and application of an indirect ELISA for detection of antibodies against emerging atypical porcine pestivirus.

Background: Atypical porcine pestivirus (APPV) is a newly discovered swine pestivirus, which can cause congenital tremor and high mortality in newborn piglets and subclinical infection in adult pigs, leading to significant impacts on the pig industry. Currently, there is no approved serological method to assess APPV infection status in pig farms.

Methods: In this study, the envelope glycoprotein E2 of APPV was highly expressed in suspension HEK293 cells, and further an indirect enzyme-linked immunosorbent assay based on the recombinant E2 protein (E2-iELISA) was developed and evaluated.

Isolation and Molecular Characterization of Atypical Porcine Pestivirus Emerging in China.

Atypical porcine pestivirus (APPV) is a recently discovered and very divergent species of the genus within the family , which causes congenital tremor (CT) in newborn piglets. In this study, an APPV epidemiological investigation was conducted by studying 975 swine samples (562 tissue and 413 serum samples) collected from different parts of China from 2017 to 2021. The results revealed that the overall positive rate of the APPV genome was 7.

Xanthones with Potential Anti-Inflammatory and Anti-HIV Effects from the Stems and Leaves of .

Four new xanthones, cratocochinones A-D (-), together with eight known analogues (-), were isolated from the stems and leaves of . The chemical structures of cratocochinones A-D (-) were elucidated by comprehensive spectroscopic analyses and the known compounds were identified by comparisons with the spectral data reported in the literature. All isolated compounds - were evaluated for their anti-inflammatory activities and anti-HIV-1 activities.

Frequency-dependent and time-variant alterations of neural activity in post-stroke depression: A resting-state fMRI study.

Background: Post-stroke depression (PSD) is one of the most frequent psychiatric disorders after stroke. However, the underlying brain mechanism of PSD remains unclarified. Using the amplitude of low-frequency fluctuation (ALFF) approach, we aimed to investigate the abnormalities of neural activity in PSD patients, and further explored the frequency and time properties of ALFF changes in PSD.

Depression circuit adaptation in post-stroke depression.

Background: Lesion locations of post-stroke depression (PSD) mapped to a depression circuit which centered by the left dorsolateral prefrontal cortex (DLPFC). However, it remains unknown whether the compensatory adaptations that may occur in this depression circuit due to the lesions in PSD.

Methods: Rs-fMRI data were collected from 82 non-depressed stroke patients (Stroke), 39 PSD patients and 74 healthy controls (HC).

[Chemical constituents from fruits of Morinda citrifolia and their inhibitory effects on proliferation of synoviocytes in vitro].

The chemical constituents from the fruits of Morinda citrifolia were systematically explored by chromatographic fractionation methods including silica gel, octadecylsilyl(ODS) gel, Sephadex LH-20 gel, and preparative high performance liquid chromatography(pre-HPLC). The chemical structures of all isolated compounds were identified on the basis of their physicochemical properties, spectroscopic analyses, as well as the comparisons of their physicochemical and spectroscopic data with the reported data in literature. As a result, 22 isolated compounds from the 90% ethanol extract of the fruits of M.

[Chemical constituents from Artocarpus incisus and their inhibitory effects on proliferation of synoviocytes in vitro].

The chemical constituents from the branches and leaves of Artocarpus incisus were isolated and purified via silica gel, ODS, and Sephadex LH-20 column chromatography as well as preparative HPLC. The chemical structures of all isolated compounds were identified in the light of their physicochemical properties, spectroscopic analyses, and comparisons of their physicochemical and spectroscopic data with the reported data in literature. As a result, 20 compounds were isolated and characterized from the 90% ethanol extract of the branches and leaves of A.

Bisabolane-type sesquiterpenoids with potential anti-inflammatory and anti-HIV activities from the stems and leaves of .

The phytochemical study on the stems and leaves of L. resulted in the isolation of a new naturally occurring bisabolane-type sesquiterpenoid, morincitrinoid A (), together with five known analogues (-). The chemical structure of was elucidated by comprehensive spectral analyses.

Syzysamalactone, an Unusual 11-Carbon δ-Lactone Derivative from the Fresh Ripe Fruits of (Wax Apple).

To study the chemical constituents from the ripe fresh fruits of (wax apple) and their potential health effects, a phytochemical investigation was undertaken. A new δ-lactone derivative, syzysamalactone (), along with a known biogenetically related δ-lactone derivative, 6-pentyl-α-pyrone (), were isolated from the fresh ripe fruits of . Syzysamalactone () is an unusual 11-carbon δ-lactone derivative, and its chemical structure and absolute configuration were elucidated by spectroscopic data analysis.

Anthraquinones with potential antiproliferative activities from the fruits of .

The phytochemical investigation on the fruits of led to the isolation and characterization of a new anthraquinone, moricitrifone (), along with seven known anthraquinones (-). The chemical structure of was elucidated by extensive spectral analyses. The known compounds (-) were identified by comparing their spectral data with those reported in the literature.

Clausanisumine, a Prenylated Bicarbazole Alkaloid from the Fruits of and Its Potential Anti-HIV Activity.

A unique prenylated bicarbazole alkaloid, clausanisumine (), and two biogenetically related known monomer carbazole alkaloids, mukonal () and 3-methylcarbazole (), were isolated from the fruits of . Clausanisumine () was an uncommon prenylated bicarbazole alkaloid, possessing an unprecedented carbon skeleton, which was composed of a simple carbazole alkaloid and a prenylated carbazole alkaloid. The chemical structure of was established by a combination of comprehensive spectral methods.

Artapilosines A and B, Unusual Phenanthrene Derivatives Related to Aporphine Alkaloids from .

Two unusual phenanthrene derivatives related to aporphine alkaloids, artapilosines A () and B (), as well as two biogenetically related known aporphine alkaloids, (-)-anonaine () and (-)--acetylanonaine (), were separated and purified from . Artapilosine A () is the first compound representative of a new class of phenanthrene derivatives having an unprecedented carbon skeleton, in which the six-membered nitrogen-containing heterocyclic structure in a typical aporphine alkaloid was substituted with a unique five-membered carbocyclic ring. This is the first report of the formation of a carbon-carbon bond between C-5 and C-6a in with the loss of the nitrogen atom N-6 in the classic aporphine alkaloid.

[Chemical constituents from Clausena excavata and their inhibitory activities against proliferation of synoviocytes].

The chemical constituents from the stems and leaves of Clausena excavata were isolated and purified by column chromatography with silica gel, ODS, Sephadex LH-20 and RP-HPLC. The chemical structures of the isolated compounds were identified on the basis of physicochemical properties, spectroscopic analysis, as well as the comparisons with the data reported in literature. Nineteen compounds were isolated from the 90% ethanol extract of the stems and leaves of C.

Cadinane-type sesquiterpenes with potential anti-inflammatory and anti-HIV activities from the stems and leaves of .

Eight cadinane-type sesquiterpenes, including a new cadinane-type sesquiterpene, named as mappianiodene (), and seven known analogues (-), were isolated and identified from the stems and leaves of . The chemical structure and absolute configurations of was elucidated by extensive spectral methods and the known compounds were identified by comparing their experimental spectral data with the reported spectral data in the literature. The anti-inflammatory and anti-HIV activities of those isolated cadinane-type sesquiterpenes were tested.

[Chemical constituents from Morinda citrifolia and their inhibitory activities on proliferation of synoviocytes in vitro].

The chemical constituents from the stems and leaves of Morinda citrifolia were isolated and purified by column chromatography methods with silica gel, ODS, Sephadex LH-20 and preparative high performance liquid chromatography(HPLC). The structures of the isolated compounds were identified by physicochemical properties and spectroscopic analysis, as well as comparisons with the data reported in literature. 17 compounds were isolated from the 90% ethanol extract of the stems and leaves of M.

Monoterpenoid indole alkaloids with potential neuroprotective activities from the stems and leaves of .

A chemical study on the stems and leaves of resulted in the isolation and identification of a new monoterpenoid indole alkaloid, melodicochine A (), together with seven known monoterpenoid indole alkaloids (-). The chemical structure of was elucidated on the basis of extensive spectral data analyses and the known compounds were identified by comparing their experimental spectral data with the reported data in the literature. All isolated indole alkaloids were evaluated for their neuroprotective effects against 6-hydroxydopamine induced cell death in human neuroblastoma SH-SY5Y cells .

Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016-2018.

Classical swine fever (CSF) is a highly contagious disease of swine caused by classical swine fever virus (CSFV). For decades the disease has been controlled in China by a modified live vaccine (C-strain) of genotype 1. The emergent genotype 2 strains have become predominant in China in the past years that are genetically distant from the vaccine strain.