[Comparison of techniques irrigating and reversing rabbit ear vein to digest, isolate and culture endothelial cells].

Objective: To establish the better method for isolating and culturing the endothelial cells (ECs).

Methods: The "irrigative digestion" and "reverse interiorly-exteriorly digestion" methods were performed for digesting, isolating, collecting and culturing the ear vein endothelial cells of rabbit. The trypan blue stain was used to test the cell activity.