[Expression of Na+-H+ exchanger 1 in human gastric carcinoma tissue and its clinical significance].

Objective: To determine the expression of Na+/H+ exchanger 1(NHE1) in human gastric carcinoma tissue and to investigate the association between NHE1 expression and clinicopathological characteristics.

Methods: The expressions of NHE1 mRNA and protein were detected in both gastric carcinoma tissue (n=60) and adjacent gastric mucosa tissue (n=30) by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The association between the expression and the clinicopathological characteristics was analyzed.

[Effects of simulated CO2 pneumoperitoneum upon function of peritoneal mesothelial cells and adherence to gastric cancer cells].

Objective: To investigate the effects of CO(2) insufflation with different pressures upon the injury, proliferation and secretion of peritoneal mesothelial cells and the possible effects of gastric cancer cells adhering to mesothelial cells.

Methods: Primary cultured peritoneal mesothelial cells were divided into CO(2) and control group. In CO(2) group, the cells were exposed to CO(2) at different pressures (10, 12 and 15 mm Hg).