The LEF-10 expression factor from the Bombyx mori nuclear polyhedrosis virus (BmNPV) does not have significant homology with other late expression factors and is thought to be a transcriptional cofactor. To investigate the function of LEF-10, a Red recombination system was used to knock out the lef-10 gene from the BmNPV genome and a lef-10 gene knockout virus (ko-Bacmid) was constructed. The lef-10 gene was repaired back to the viral genome using a Bac-to-Bac system to create the repaired virus (re-Bacmid).
View Article and Find Full Text PDFThis study compared the difference in microbial community and power generation capacity of air-cathode MFCs enriched under anode aerobic and anaerobic conditions. Results showed that MFCs successfully started with continuous air inputting to anode chamber. The aerobic enriched MFC produced comparable and even more electricity with the fuels of acetate, glucose and ethanol compared to the anaerobic MFC when returning to anaerobic condition.
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