A Simplified and Turn-on Fluorescence Chemosensor Based on Coumarin Derivative for Detection of Aluminium(III) Ion in Pure Aqueous Solution.

A novel chemosensor L based on coumarin Schiff-base was synthesized and investigated. Sensor L showed remarkable selectivity for Al(3+) in Tris-HCl aqueous buffer solution (pH 7.2), and the selectivity was not affected by the presence of a large excess of other competitive ions.

First and facile enzymatic synthesis of β-fucosyl-containing disaccharide nucleosides through β-galactosidase-catalyzed regioselective glycosylation.

β-Galactosidase from bovine liver was purified to homogeneity. Its molecular mass was estimated to be 54kDa by SDS-PAGE, 60kDa by gel permeation chromatography, and 57kDa by matrix-assisted laser desorption ionization - time of flight tandem mass spectrum. This enzyme displayed the highest catalytic efficiency with p-nitrophenyl β-d-galactopyranoside (Vmax/Km value, 0.

[In vitro study of the secondary mesiobuccal canal of the maxillary second molar].

Objective: To get some anatomical knowledge about the second mesiobuccal canal (MB2) of the maxillary second molar of Shandong region by studying the teeth in vitro. The anatomical knowledge may help us to improve the successful rate of root canal therapy of the maxillary molar.

Methods: 118 maxillary second molars were collected from the different region in Shandong province.

[Detecting ABO blood type of bloodstain with fluorescent antibody method].

Unlabelled: OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.

Methods: According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.

Results: The fluorescent antibody method is highly sensitive, accurate and simple.