Comparison of biological behavior of lacrimal gland adenoid cystic carcinoma with high-grade transformation cells.

Aim: To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation (LACC-HGT) primary cells cultured by high-grade transformation tissue and non-high-grade transformation (non-HGT) primary cells cultured by non-high-grade transformation tissue in proliferation, metastasis, drug susceptibility, and genes.

Methods: LACC-HGT primary cells were established by tissue block culture, and the 4 to 10 generation primary cells were selected as research objects. The cells were preliminarily identified by immunofluorescent staining.

[The advance of β-blockers in the treatment of infantile hemangiomas].

Infantile hemangiomas (IH) is the most common benign tumour in early infancy.IH often grows under skins and subcutaneous tissues around head and neck, but it is not rare in ophthalmologic clinical work.IH in the eyelids, orbit and other organs, characterized by their very rapid growth, can cause severe complications, including ptosis and translocation of eyeball.

[Primary culture and morphological characteristics of human lacrimal adenoid cystic carcinoma cells].

Objective: To explore a method of primary culturing human adenoid cystic carcinoma cells of lacrimal gland.

Methods: Experiment research. Tumor tissue was obtained from the surgical material of a patient diagnosed as lacrimal adenoid cystic carcinoma in Tianjin Medical University Eye Hospital during May 16th to June 1st.

[Overexpression of the receptor tyrosine kinase EphA2 in choroidal melanoma: correlation with vesculogenic mimicry and prognosis].

Objective: To study the correlation of EphA2 protein expression with vesculogenic mimicry (VM), clinicopathological characteristics and prognosis in choroidal melanoma (CM).

Methods: It was a retrospective case series study. Between January 1992 and December 2005, 56 cases of human CM with clinicopathologic data from the Second Hospital of Tianjin Medical University were studied.

[The application of serum free medium in the research of isolating cancer stem cells].

The isolation and identification of cancer stem cells are the key points in exploring characteristics of cancer stem cells at present. Several species of cancer stem cells, for instance, retinoblastoma tumor stem cells, cancer stem cells of melanoma of choroid, breast cancer stem cells, lung cancer stem cells, colon cancer stem cells, etc., have been isolated and cultured successfully by serum free medium while their biological functions and characteristics are acquired.

[Analysis of expression of cancer stem cell-related markers in orbital adenoid cystic carcinoma].

Objective: To observe the expression and distribution of CD44, CD133, and ABCG2 in orbital adenoid cystic carcinoma (ACC) and investigate their correlations with pathological type and prognosis.

Method: Two steps method of immunohistochemical staining was employed in 33 cases of paraffin embedded surgical specimens of human orbital ACC, 5 cases of recurrence samples, 3 cases of an excised lacrimal gland caused by neither inflammation nor tumor diseases, and 6 cases of xenograft tumors in nude mice. A retrospective analysis was performed on the clinical material of these patients, which were collected from Jan.

[VCR-loaded nanoparticles as targeted delivery system for the treatment of orbital adenoid cystic carcinoma].

Objective: To prepare and observe the properties of Folate Receptor-mediated VCR-loaded nanoparticles, which is abbreviated as FA-PLGA (VCR)-NP and to study the inhibitory effect of FA-PLGA (VCR)-NP in ACC-2 cells in vitro and in ACC in BALB/c-nu mice.

Methods: The mAified W/O/W extraction-evaporation technique was chosen to prepare FA-PLGA (VCR)-NP. Tumor cells were divided into three groups: VCR, PLGA (VCR)-NP and FA-PLGA (VCR)-NP.

Inhibition of tumor growth and vasculogenic mimicry by curcumin through down-regulation of the EphA2/PI3K/MMP pathway in a murine choroidal melanoma model.

This study aims to investigate the underlying mechanism by which curcumin inhibits tumor growth and reduces vasculogenic mimicry (VM) in a murine choroidal melanoma model. Sixty mice were given subretinal injection with B16F10 cells and divided into a treatment and a control group. Curcumin was administered to the treatment group once a day at a dose of 100 mg/kg for 18 days starting at d3 (the day of inoculation is designated as d0); an equivalent volume of poloxamer-F68 was administered to the control group.

[Clinical analysis of primary extraocular muscles tumors].

Objective: To analyze the clinical manifestation of primary extraocular muscles tumors and to study the diagnosis and therapy of this disorder.

Methods: Retrospective analysis of the clinical data of all primary extraocular muscles tumors (the diagnosis was confirmed by operation and pathological examination) in our hospital.

Results: There were 9 cases of primary extraocular muscle tumors.

[The mechanisms of microenvironments influence on vasculogenic mimicry between intraocular and subcutaneous melanoma].

Objective: To investigate the influence of different microenvironments on melanoma vasculogenic mimicry, invasiveness and metastasis behavior.

Methods: It was an experimental study. Sixty C57BL/6J mice were randomly divided into two groups with 30 mice per group.

[Etiology, diagnosis and treatment analysis of 37 cases with orbital fistula].

Objective: To present some instruction in diagnosis and treatment of the orbital fistula by analyze the clinical manifestation.

Method: The clinical data for 37 cases with orbital fistula treated during 1980 to 2007 were analyzed retrospectively.

Results: Among 37 cases with orbital fistula, there are 21 cases caused by foreign bodies, including 17 cases by retained wooden bodies, 3 cases by iatrogenic foreign bodies and 1 case by firecrackers.

[Analysis of treatment and prognosis of orbital adenoid cystic carcinoma].

Objective: To summarize the treatment outcomes of orbital adenoid cystic carcinoma and to evaluate prognostic factors.

Method: A retrospective case series study was performed on 75 patients with orbital adenoid cystic carcinoma treated from 1991 to 2006.

Results: The 2- and 5-year local recurrence rate of solid type orbital adenoid cystic carcinoma was significantly higher than that of the adeno-tubiform type [2-year, 85% (17/20) vs 23.

[The ophthalmic applications of nanotechnology].

This paper briefly reviews the researches and applications of nanotechnology in ophthalmological field recent years. Nanotechnology is the production of modern science and advanced engineering. In ophthalmological field it is generally applied on foundational investigation, medicine carrying agent, biomaterial, diagnosis and therapy.

[Comprehensive therapy of orbital tumor].

Although the popularization of the knowledge of orbital disease is increasing, some issues such as surgery complications, recurrence, metastases may significantly affect the outcome of treatment. The new concept of comprehensive therapy offers an integrated individual therapy to patients. Application the concept in clinical practice is a key measure to improve the cured rate.

[Study on differential diagnosis between MALT-type lymphoma and reactive lymphoid hyperplasia of the ocular adnexa].

Objective: To study the methods of differential diagnosis between mucosa-associated lymphoid tissue (MALT) type lymphoma and reactive lymphoid hyperplasia in ocular adnexa.

Methods: The clinical, morphologic, immunohistochemical, and molecular biologic (polymerase chain reaction [PCR]) studies were performed on 29 patients with MALT-type lymphoma and 8 patients with reactive lymphoid hyperplasia of the ocular adnexa.

Results: 29 cases with MALT-type lymphoma expressed B-cell markers and but T-cell makers, whereas 8 cases of reactive lymphoid hyperplasia all expressed B and T cell markers and the positive rate of T-cell marker was up to 30%.

[The clinical significance of calcification in orbital computed tomography].

Objective: To study clinic significance of ocular and orbital diseases with computed tomographic (CT) scans.

Methods: 2181 ocular and orbital photos of computed tomographic scans were reviewed. The relevance with pathogenesis, diagnosis criteria and differential diagnosis of calcification was analyzed.

[Clinical analysis of orbital histocytosis X].

Objective: To study the clinical and imaging features together with diagnosis and management of orbital histocytosis X.

Methods: In a retrospective study from 1973 to 1999, 47 cases of histocytosis X were reviewed related with clinical materials, including eosinophilic granuloma 17 cases and Hand-Schuller- Christian disease 30 cases.

Results: Most occur in children and teenagers, mainly in male.