[Effects of different organic fertilizers on the microbes in rhizospheric soil of flue-cured tobacco].

A field experiment was conducted to study the effects of applying different organic fertilizers (refined organic fertilizer and bio-organic fertilizer) and their combination with 20% reduced chemical fertilizers on the microbes in rhizospheric soil of flue-cured tobacco, the resistance of the tobacco against bacterial wilt, and the tobacco yield and quality. As compared with conventional chemical fertilization (CK), applying refined organic fertilizer (ROF) or bio-organic fertilizer (BIO) in combining with 20% reduced chemical fertilization increased the bacterial number and the total microbial number in the rhizospheric soil significantly. Applying BIO in combining with 20% reduced chemical fertilization also increased the actinomyces number in the rhizospheric soil significantly, with an increment of 44.

Obligate mutualism within a host drives the extreme specialization of a fig wasp genome.

Background: Fig pollinating wasps form obligate symbioses with their fig hosts. This mutualism arose approximately 75 million years ago. Unlike many other intimate symbioses, which involve vertical transmission of symbionts to host offspring, female fig wasps fly great distances to transfer horizontally between hosts.

Low CADM2 expression predicts high recurrence risk of hepatocellular carcinoma patients after hepatectomy.

Purpose: To investigate the expression and clinical significance of CADM2 in hepatocellular carcinomas (HCC).

Methods: The level of expression of CADM2 mRNA was assessed in frozen tumor specimens and adjacent noncancerous tissues from 30 HCC patients by real-time PCR. The protein level was determined by immunohistochemistry on a tissue microarray containing tumor and adjacent noncancerous tissues from 234 HCC patients.

Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides.

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1(T), was isolated from Takakia lepidozioides collected from Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain RG1-1(T) were iso-C(15 : 0) (19.8%), summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c, 17.

Stat3 inhibits PTPN13 expression in squamous cell lung carcinoma through recruitment of HDAC5.

Proteins of the protein tyrosine phosphatase (PTP) family are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, and apoptosis. PTPN13 (also known as FAP1, PTPL1, PTPLE, PTPBAS, and PTP1E), a putative tumor suppressor, is frequently inactivated in lung carcinoma through the loss of either mRNA or protein expression. However, the molecular mechanisms underlying its dysregulation have not been fully explored.

Cyclosporin A promotes proliferating cell nuclear antigen expression and migration of human cytotrophoblast cells via the mitgen-activated protein kinase-3/1-mediated nuclear factor-κB signaling pathways.

Our previous studies have demonstrated that cyclosporin A (CsA) promotes the proliferation and migration of human trophoblasts via the mitgen-activated protein kinase-3/1 (MAPK3/1) pathway. In the present study, we further investigated the role of nuclear factor (NF)-κB in the CsA-induced trophoblast proliferating cell nuclear antigen (PCNA) expression and migration, and its relationship to MAPK3/1 signal. Flow cytometry was used to analyze the expression of PCNA in trophoblasts.

Assessment of pyrrolizidine alkaloid-induced toxicity in an in vitro screening model.

Ethnopharmacological Relevance: Pyrrolizidine alkaloids (PAs) are a group of heterocyclic phytotoxins present in a wide range of plants. The consumption of PA-containing medicinal herbs or PA-contaminated foodstuffs has long been reported to cause human hepatotoxicity. However, the degrees of hepatotoxicity of different PAs are unknown, which makes it difficult to determine a universal threshold of toxic dose of individual PAs for safe regulation of PA-containing natural products.

Value of the frontal planar QRS-T angle on cardiac dysfunction in patients with old myocardial infarction.

Objectives: To explore a method of surface electrocardiogram for assessing cardiac dysfunction in patients with old myocardial infarction.

Methods: 1000 patients with old myocardial infarction in Anzhen hospital were analyzed retrospectively. The planar QRS-T angle was calculated automatically according to QRS-wave and T-wave vectors by the electrocardiogram machine.

Identification of genuine primary pulmonary NK cell lymphoma via clinicopathologic observation and clonality assay.

Unlabelled: Extranodal natural killer (NK)/T-cell lymphoma, nasal type, is an uncommon lymphoma associated with the Epstein-Barr virus (EBV). It most commonly involves the nasal cavity and upper respiratory tract. Primary pulmonary NK/T cell lymphoma is extremely rare.

Antioxidant phenolic glycoside and flavonoids from Pieris japonica.

A new phenolic glycoside, benzyl 2-hydroxy-4-O-β-D-glucopyranosyl-benzoate (1), along with nine known flavonoids, epicatechin-(2 → O → 7,4 → 8)-ent-epicatechin (2), bis-8,8'-catechinylmethane (3), quercetin (4), quercetin-3-O-α-L-arabinfuranoside (5), quercetin-3-O-α-L-rhamnopyranoside (6), astilbin (7), engeletin (8), (2S,3R)-ent-catechin (9), and 2',4-dihydroxy-4'-methoxy-6'-O-β-D-glucopyranosyl dihydrochalcone (10), was isolated from the flowers of Pieris japonica. Their structures were elucidated on the basis of MS, 1D NMR, and 2D NMR techniques. This paper describes the isolation, structural elucidation as well as in vitro antioxidant activity of these compounds.

[Differences in seed kernel quality and related enzyme activities of different quality type peanut cultivars].

Taking high-protein peanut cultivar KB008, high-fat peanut cultivar Hual7 (H17), and high-oleic acid/linoleic acid (O/L) peanut cultivar Nongda818 as test materials, a field experiment was conducted in 2010 and 2011 to study the differences in the contents of protein, fat, and their components of the seed kernels, and the differences in the activities of the carbon and nitrogen metabolism enzymes related to the quality synthesis in the blades. As compared with that of H17 and Nongda818, the seed kernel of KB008 had significantly higher protein content but significantly lower soluble sugar content and O/L ratio, and the contents of the amino acid components, especially glutamic acid and lysine, in the seed kernel of KB008 were significantly higher. During the whole growth period, the activities of nitrate reductase (NR) , glutamine.

[Inhibitory effects of luteolin on human gastric carcinoma xenografts in nude mice and its mechanism].

Objective: To explore the in vivo anticancer effects of luteolin with BGC-823 gastric carcinoma xenografts in nude mice and elucidate its mechanism.

Methods: After modeling of gastric carcinoma xenografts in nude mice, 40 BALB/c (nu/nu) nude mice were randomly divided into 5 groups (n = 8 each). And an intraperitoneal injection of luteolin was administered at 10 mg/kg (low-dose), 20 mg/kg (middle-dose) and 40 mg/kg (high-dose) groups.

Aspirin use and risk of age-related macular degeneration: a meta-analysis.

Background: Age-related macular degeneration (AMD) is the main cause of blindness and the curative options are limited. The objective of this meta-analysis was to determine the association between aspirin use and risk of AMD.

Methods: A comprehensive literature search was performed in PubMed, Embase, Web of Science, and reference lists.

Comparison of the diversity of root-associated bacteria in Phragmites australis and Typha angustifolia L. in artificial wetlands.

Common reed (Phragmites australis) and narrow-leaved cattail (Typha angustifolia L.) are two plant species used widely in artificial wetlands constructed to treat wastewater. In this study, the community structure and diversity of root-associated bacteria of common reed and narrow-leaved cattail growing in the Beijing Cuihu Wetland, China, were investigated using 16S rDNA library and PCR-denaturing gradient gel electrophoresis methods.

Interleukin 35: protective role and mechanism in type 1 diabetes.

Interleukin 35 (IL-35), a cytokine secreted by regulatory T (Treg) cells from the differentiation of conventional CD4 T cells, is a member of the IL-12 family. The IL-12 family of cytokines exhibits an anti-inflammatory property. IL-35 has recently been shown to influence the immune modulation in various diseases, including inflammatory bowel disease, Graves' disease, rheumatoid arthritis, colitis, psoriasis, and type 1 diabetes (T1D).

Survivin selective inhibitor YM155 induce apoptosis in SK-NEP-1 Wilms tumor cells.

Background: Survivin, a member of the family of inhibitor of apoptosis proteins, functions as a key regulator of mitosis and programmed cell death. YM155, a novel molecular targeted agent, suppresses survivin, which is overexpressed in many tumor types. The aim of this study was to determine the antitumor activity of YM155 in SK-NEP-1 cells.

Insulin resistance and adiponectin levels are associated with height catch-up growth in pre-pubertal Chinese individuals born small for gestational age.

Background And Objective: The study was performed to determine whether catch-up growth is associated with the development of insulin resistance and to explore serum endocrine markers associated with the metabolism of adipose tissue in a Chinese population born small for gestational age(SGA) SUBJECTS AND METHODS: We recruited 56 children born SGA with catch-up growth and 55 born without catch-up growth, who were further grouped into groups I (with BMI catch-up) and II (without BMI catch-up) respectively, as well as 52 children born appropriate for gestational age (AGA) with normal height. Their serum fasting insulin, fasting glucose, insulin-like growth factor-1, adiponectin, IGFBP-1, triglyceride concentrations, and the homeostasis assessment model for insulin resistance (HOMA-IR) were evaluated.

Results: (1) The HOMA-IR values in SGA-I with catch-up growth group were significantly higher than those in SGA-II with catch-up growth, SGA-I without catch-up growth and AGA children respectively.

Indocyanine green-assisted internal limiting membrane peeling in macular hole surgery: a meta-analysis.

Background: The opinion of application of indocyanine green (ICG) in the macular hole surgery was contradictory. Here we conducted a meta-analysis to evaluate the effect of in internal limiting membrane (ILM) peeling for macular hole surgery.

Methods And Findings: We searched electronic databases for comparative studies published before July 2012 of ILM peeling with and without ICG.

Inhibition of the ecto-beta subunit of F1F0-ATPase inhibits proliferation and induces apoptosis in acute myeloid leukemia cell lines.

Background: Leukemia, a heterogeneous clonal disorder of hematopoietic progenitor cells, presents a world-wide health problem, especially in childhood. F1F0 ATPase, an inner mitochondrial enzyme, is expressed on the plasma membrane of tumor cells, and its inhibition induces both anti-angiogenic and anti-tumorigenic activity.

Methods: Monoclonal Antibody (McAb) against ATPase was produced by polyethylene glycol-mediated fusions and screened by ELISA.

Burkholderia grimmiae sp. nov., isolated from a xerophilous moss (Grimmia montana).

A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain R27(T), was isolated from the moss Grimmia montana, collected from Beijing Songshan National Nature Reserve, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain R27(T) were C18:1ω7c (33.6%), C16:0 (16.

Effects of a high-protein diet on insulin resistance and body fat in catch-up growth rats born small for gestational age.

Background/aim: This study was designed to evaluate the effects of a high-protein (HP) diet on insulin resistance and body fat in catch-up growth (CUG) rats born small for gestational age (SGA).

Methods: SGA rats were randomly divided into standard diet and HP diet groups. Perirenal fat weight and blood glucose, serum insulin and insulin-like growth factor-1 levels were measured at 4 and/or 8 weeks.

Primary pulmonary malignant melanoma: a clinicopathologic study of two cases.

Unlabelled: Malignant melanoma involving the respiratory tract is nearly always metastatic in origin, and primary tumors are very rare. To our knowledge, about 30 cases have been reported in the English literature, one of which involved multiple brain metastases. Here, we report two cases of primary pulmonary malignant melanoma.

Survival analysis of late-onset systemic lupus erythematosus: a cohort study in China.

The aim of this study is to explore the survival rate and risk factors of mortality in patients with late-onset systemic lupus erythematosus (SLE) in a large cohort. Clinical presentations, disease activity, organ damage scores, autoantibody profile, and mortality data were obtained retrospectively from late-onset SLE patients (onset age ≥50 years) diagnosed between 1995 and 2009. The risk factors of organ damage were evaluated by the chi-square test and logistic regression.

Analyzing the gene expression profile of pediatric acute myeloid leukemia with real-time PCR arrays.

Background: The Real-time PCR Array System is the ideal tool for analyzing the expression of a focused panel of genes. In this study, we will analyze the gene expression profile of pediatric acute myeloid leukemia with real-time PCR arrays.

Methods: Real-time PCR array was designed and tested firstly.