A novel digestive protease chymotrypsin-like serine contributes to anti-BmNPV activity in silkworm (Bombyxmori).

Serine proteases (SPs) are important proteases in the digestive system of lepidopteran insects. They play important roles in protein digestion, coagulation, signal transduction, hormone activation, inflammation and development. Blood-borne pyosis caused by Bombyx mori nuclear polyhedrosis virus (BmNPV) has caused serious harm to sericulture.

Gold Nanoparticle-Induced Photocurrent Quenching and Recovery of Polymer Dots: Toward Signal-On Energy-Transfer-Based Photocathodic Bioanalysis of Telomerase Activity in Cell Extracts.

Energy transfer (ET) in photoelectrochemical (PEC) bioanalysis is usually generated between noble metal nanoparticles (NPs) and traditional inorganic quantum dots (QDs). Using the innovative polymer dot (Pdot)-involved ET, this work reports the first signal-on and cathodic PEC bioanalysis toward telomerase (TE) activity in cell extracts. Specifically, the sequential binding of capture DNA (cDNA), telomerase primer sequence (TS), and Au NP-labeled probe DNA (Au NP-pDNA) on the electrode would place the Au NPs in close proximity of the Pdots, leading to obvious quenching of the cathodic photocurrent.

Binding-induced formation of DNAzyme on an Au@Ag nanoparticles/TiO nanorods electrode: Stimulating biocatalytic precipitation amplification for plasmonic photoelectrochemical bioanalysis.

Photoelectrochemical (PEC) DNA bioanalysis has been drawing more attention in recent years due to the advantages of PEC technique and the vital importance of DNA biomolecules. DNAzymes are unique catalytic nucleic acid molecules that are capable of catalyzing specific biochemical reactions. Using the target-binding-induced conformation change of hairpin DNA probe to hemin/G-quadruplex-based DNAzyme and a plasmonic Au@Ag nanoparticles (NPs)/TiO nanorods (NRs)/fluorine-doped tin oxide (FTO) heterostructured photoelectrode, this work reported a novel and sensitive PEC DNA analysis on the basis of a DNAzyme-stimulated biocatalytic precipitation (BCP) strategy.

Semiconducting CuO Nanotubes: Synthesis, Characterization, and Bifunctional Photocathodic Enzymatic Bioanalysis.

This work reports the synthesis, characterization, and application of bifunctional semiconducting CuO nanotubes (NTs) electrode for innovative synergized cathodic photoelectrochemical (PEC) enzymatic bioanalysis. Specifically, CuO NTs electrode was fabricated by surface oxidation of the copper foil in an alkaline aqueous solution with (NH)SO and then annealed in air at 200 °C. After the subsequent coupling with the model enzyme of xanthine oxidase (XOD), the resulted photocathodic enzyme biosensor exhibited good analytical performance of rapid response, high stability, and good sensitivity.

Hierarchical CuInS-based heterostructure: Application for photocathodic bioanalysis of sarcosine.

In this study, on the basis of hierarchical CuInS-based heterostructure, a novel cathodic photoelectrochemical (PEC) enzymatic bioanalysis of the sarcosine detection was reported. Specifically, heterostructured CuInS/NiO/ITO photocathode was prepared and sarcosine oxidases (SOx) were integrated for the construction of the enzymatic biosensor. In the bioanalysis, the O-dependent suppression of the cathodic photocurrent can be observed due to the competition between the as-fabricated O-sensitive photocathode and the SOx-catalytic event toward O reduction.

Bismuth Oxyiodide Couples with Glucose Oxidase: A Special Synergized Dual-Catalysis Mechanism for Photoelectrochemical Enzymatic Bioanalysis.

On the basis of a special synergized dual-catalysis mechanism, this work reports the preparation of a BiOI-based heterojunction and its use for cathodic photoelectrochemical (PEC) oxidase biosensing, which, unexpectedly, revealed that hydrogen peroxide (HO) had a greater impact than dioxygen (O). Specifically, the BiOI layer was in situ formed on the substrate through an impregnating hydroxylation method for the following coupling with the model enzyme of glucose oxidases (GOx). The constructed cathodic PEC enzyme sensor exhibited a good analytical performance of rapid response, high stability, and good selectivity.

Ag nanoclusters could efficiently quench the photoresponse of CdS quantum dots for novel energy transfer-based photoelectrochemical bioanalysis.

Herein the influence of ultrasmall Ag nanoclusters (Ag NCs) against CdS quantum dots (QDs) in a photoelectrochemical (PEC) nanosystem was exploited for the first time, based on which a novel PEC bioanalysis was successfully developed via the efficient quenching effect of Ag NCs against the CdS QDs. In a model system, DNA assay was achieved by using molecular beacon (MB) probes anchored on a CdS QDs modified electrode, and the MB probes contain two segments that can hybridize with both target DNA sequence and the label of DNA encapsulated Ag NCs. After the MB probe was unfolded by the target DNA sequence, the labels of oligonucleotide encapsulated Ag NCs would be brought in close proximity to the CdS QDs electrode surface, and efficient photocurrent quenching of QDs could be resulted from an energy transfer process that originated from NCs.

Sulfonation of ordered mesoporous carbon supported Pd catalysts for formic acid electrooxidation.

A novel supporting material containing benzenesulfonic acid (BSA) groups and ordered mesoporous carbons (OMCs) was first prepared by in situ radical polymerization of 4-styrenesulfonate and isoamyl nitrite under ambient conditions. Then, Pd nanoparticles were deposited on as-produced OMCs (f-OMCs) by the NaBH(4) reduction method. The structure and nature of the resulting composites were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and nitrogen adsorption-desorption.

Synthesis and electrochemical properties of chemically substituted LiMn2O4 prepared by a solution-based gel method.

Lithium manganese oxide, LiMn(2)O(4), and its substituted samples LiM(0.05)Mn(1.95)O(4) (M=Al, Co, and Zn) were first prepared by a cost-saving and effective new solution-based gel method using a mixture of acetate and ethanol as the chelating agent.

Enhancement of the electrochemical properties of LiMn2O4 through Al3+ and F- co-substitution.

The cathode-active materials LiMn2O4, LiAl0.1Mn1.9O4, and LiAl0.

Preparation of novel nano-composite Ni(OH)2/USY material and its application for electrochemical capacitance storage.

A novel nano-composite material of Ni(OH)2/USY was prepared in our lab. This nanostructure creates electrochemical accessibility of electrolyte OH- ions to Ni(OH)2 thin layers and a fast diffusion rate within the redox phase.